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Association of short tandem repeat polymorphism in the promoter of prostate cancer antigen 3 gene with the risk of prostate cancer.

Zhou W, Chen Z, Hu W, Shen M, Zhang X, Li C, Wen Z, Wu X, Hu Y, Zhang X, Duan X, Han X, Tao Z - PLoS ONE (2011)

Bottom Line: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135).Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test.The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang, People's Republic of China.

ABSTRACT

Background: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In our study, we evaluated whether there is polymorphism in PCA3 promoter region and also assess the association of the polymorphism with prostate cancer.

Methodology/principal findings: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135). Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test. Possible deviation of the genotype frequencies from controls and PCa cases expected under Hardy-Weinberg equilibrium was assessed by the chi-square test. Short tandem repeat polymorphism of TAAA was found in the promoter region of PCA3 gene, five polymorphisms and eight genotypes were identified. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. The group 11TAAA and ≥12TAAA were associated with higher relative risk for prostate cancer than group ≤10TAAA (OR = 1.76, 95%CI = 1.07-2.89[for group 11TAAA]; OR = 5.28, 95%CI = 1.76-15.89[for group ≥12TAAA]).

Conclusions/significance: The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population.

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Related in: MedlinePlus

Repressentative PCR -based cloning and sequencing of STR polymorphism in the promoter region of PCA3 gene.A. (TAAA)4 alleles; B. (TAAA)5 alleles; C. (TAAA)6 alleles; D. (TAAA)7 alleles; E. (TAAA)8 alleles.
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pone-0020378-g001: Repressentative PCR -based cloning and sequencing of STR polymorphism in the promoter region of PCA3 gene.A. (TAAA)4 alleles; B. (TAAA)5 alleles; C. (TAAA)6 alleles; D. (TAAA)7 alleles; E. (TAAA)8 alleles.

Mentions: In the present study, five polymorphisms were identified: 4, 5, 6, 7, 8 (the number represents the repeat times of TAAA in the promoter of PCA3 gene, Fig 1), and eight genotypes were founded. The 4/5, 4/6, 5/5, 5/6, 5/7, 5/8, 6/6, 6/7 genotypes were observed in 0.5%, 0.5%, 52.7%, 34.4%, 3.3%, 1.6%, 5.4% and 1.6% of PCa patients, respectively. Whereas only the 5/5, 5/6, 6/6genotypes were observed in 71.1%, 25.8%, 3.1% of control subjects, respectively. There was no evidence that the genotype frequencies deviated from those expected under the Hardy-Weinberg equilibrium for patients (χ2 = 1.44, df = 3, P = 0.70) and control subjects (χ2 = 0.14, df = 1, P = 0.71).


Association of short tandem repeat polymorphism in the promoter of prostate cancer antigen 3 gene with the risk of prostate cancer.

Zhou W, Chen Z, Hu W, Shen M, Zhang X, Li C, Wen Z, Wu X, Hu Y, Zhang X, Duan X, Han X, Tao Z - PLoS ONE (2011)

Repressentative PCR -based cloning and sequencing of STR polymorphism in the promoter region of PCA3 gene.A. (TAAA)4 alleles; B. (TAAA)5 alleles; C. (TAAA)6 alleles; D. (TAAA)7 alleles; E. (TAAA)8 alleles.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105025&req=5

pone-0020378-g001: Repressentative PCR -based cloning and sequencing of STR polymorphism in the promoter region of PCA3 gene.A. (TAAA)4 alleles; B. (TAAA)5 alleles; C. (TAAA)6 alleles; D. (TAAA)7 alleles; E. (TAAA)8 alleles.
Mentions: In the present study, five polymorphisms were identified: 4, 5, 6, 7, 8 (the number represents the repeat times of TAAA in the promoter of PCA3 gene, Fig 1), and eight genotypes were founded. The 4/5, 4/6, 5/5, 5/6, 5/7, 5/8, 6/6, 6/7 genotypes were observed in 0.5%, 0.5%, 52.7%, 34.4%, 3.3%, 1.6%, 5.4% and 1.6% of PCa patients, respectively. Whereas only the 5/5, 5/6, 6/6genotypes were observed in 71.1%, 25.8%, 3.1% of control subjects, respectively. There was no evidence that the genotype frequencies deviated from those expected under the Hardy-Weinberg equilibrium for patients (χ2 = 1.44, df = 3, P = 0.70) and control subjects (χ2 = 0.14, df = 1, P = 0.71).

Bottom Line: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135).Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test.The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang, People's Republic of China.

ABSTRACT

Background: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In our study, we evaluated whether there is polymorphism in PCA3 promoter region and also assess the association of the polymorphism with prostate cancer.

Methodology/principal findings: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135). Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test. Possible deviation of the genotype frequencies from controls and PCa cases expected under Hardy-Weinberg equilibrium was assessed by the chi-square test. Short tandem repeat polymorphism of TAAA was found in the promoter region of PCA3 gene, five polymorphisms and eight genotypes were identified. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. The group 11TAAA and ≥12TAAA were associated with higher relative risk for prostate cancer than group ≤10TAAA (OR = 1.76, 95%CI = 1.07-2.89[for group 11TAAA]; OR = 5.28, 95%CI = 1.76-15.89[for group ≥12TAAA]).

Conclusions/significance: The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population.

Show MeSH
Related in: MedlinePlus