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Forebrain NR2B overexpression facilitating the prefrontal cortex long-term potentiation and enhancing working memory function in mice.

Cui Y, Jin J, Zhang X, Xu H, Yang L, Du D, Zeng Q, Tsien JZ, Yu H, Cao X - PLoS ONE (2011)

Bottom Line: Its functions are associated with NMDA receptors.The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP.Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Brain Functional Genomics, The Key Laboratory of MOE, East China Normal University, Shanghai, China.

ABSTRACT
Prefrontal cortex plays an important role in working memory, attention regulation and behavioral inhibition. Its functions are associated with NMDA receptors. However, there is little information regarding the roles of NMDA receptor NR2B subunit in prefrontal cortical synaptic plasticity and prefrontal cortex-related working memory. Whether the up-regulation of NR2B subunit influences prefrontal cortical synaptic plasticity and working memory is not yet clear. In the present study, we measured prefrontal cortical synaptic plasticity and working memory function in NR2B overexpressing transgenic mice. In vitro electrophysiological data showed that overexpression of NR2B specifically in the forebrain region resulted in enhancement of prefrontal cortical long-term potentiation (LTP) but did not alter long-term depression (LTD). The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP. In addition, NR2B transgenic mice exhibited better performance in a set of working memory paradigms including delay no-match-to-place T-maze, working memory version of water maze and odor span task. Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory.

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Synaptosome NR2B-receptor Protein in Prefrontal Cortex.Analysis of OD value show that the relative quantity of NR2B-receptor protein of prefrontal cortex in Tg and Wt mice is 0.5±0.1 and 0.13±0.02, respectively (p<0.05, Student's t-test).
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pone-0020312-g002: Synaptosome NR2B-receptor Protein in Prefrontal Cortex.Analysis of OD value show that the relative quantity of NR2B-receptor protein of prefrontal cortex in Tg and Wt mice is 0.5±0.1 and 0.13±0.02, respectively (p<0.05, Student's t-test).

Mentions: Using western blot techniques, we first measured the NR2B protein of synaptosomal membrane fractions prepared from the prefrontal cortex of NR2B transgenic and wild type mice. Our western blot results showed that there was an enhanced expression of NR2B protein in the synaptic membrane of prefrontal cortex of transgenic mice compared to the wild type mice (Figure 2).


Forebrain NR2B overexpression facilitating the prefrontal cortex long-term potentiation and enhancing working memory function in mice.

Cui Y, Jin J, Zhang X, Xu H, Yang L, Du D, Zeng Q, Tsien JZ, Yu H, Cao X - PLoS ONE (2011)

Synaptosome NR2B-receptor Protein in Prefrontal Cortex.Analysis of OD value show that the relative quantity of NR2B-receptor protein of prefrontal cortex in Tg and Wt mice is 0.5±0.1 and 0.13±0.02, respectively (p<0.05, Student's t-test).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105019&req=5

pone-0020312-g002: Synaptosome NR2B-receptor Protein in Prefrontal Cortex.Analysis of OD value show that the relative quantity of NR2B-receptor protein of prefrontal cortex in Tg and Wt mice is 0.5±0.1 and 0.13±0.02, respectively (p<0.05, Student's t-test).
Mentions: Using western blot techniques, we first measured the NR2B protein of synaptosomal membrane fractions prepared from the prefrontal cortex of NR2B transgenic and wild type mice. Our western blot results showed that there was an enhanced expression of NR2B protein in the synaptic membrane of prefrontal cortex of transgenic mice compared to the wild type mice (Figure 2).

Bottom Line: Its functions are associated with NMDA receptors.The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP.Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Brain Functional Genomics, The Key Laboratory of MOE, East China Normal University, Shanghai, China.

ABSTRACT
Prefrontal cortex plays an important role in working memory, attention regulation and behavioral inhibition. Its functions are associated with NMDA receptors. However, there is little information regarding the roles of NMDA receptor NR2B subunit in prefrontal cortical synaptic plasticity and prefrontal cortex-related working memory. Whether the up-regulation of NR2B subunit influences prefrontal cortical synaptic plasticity and working memory is not yet clear. In the present study, we measured prefrontal cortical synaptic plasticity and working memory function in NR2B overexpressing transgenic mice. In vitro electrophysiological data showed that overexpression of NR2B specifically in the forebrain region resulted in enhancement of prefrontal cortical long-term potentiation (LTP) but did not alter long-term depression (LTD). The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP. In addition, NR2B transgenic mice exhibited better performance in a set of working memory paradigms including delay no-match-to-place T-maze, working memory version of water maze and odor span task. Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory.

Show MeSH
Related in: MedlinePlus