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MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.

Ferreira L, Sánchez-Juanes F, García-Fraile P, Rivas R, Mateos PF, Martínez-Molina E, González-Buitrago JM, Velázquez E - PLoS ONE (2011)

Bottom Line: Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae.Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences.The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, Spain.

ABSTRACT
Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae. In this work we evaluated the suitability of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for this purpose. Firstly, we evaluated the capability of this methodology to differentiate among species of family Rhizobiaceae including those closely related and then we extended the database of MALDI Biotyper 2.0 including the type strains of 56 species from genera Rhizobium, Ensifer and Shinella. Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences. The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.

Show MeSH
MALDI-TOF MS spectra of whole-cell extracts obtained from the type strains of two close and one divergent species from each genus analysed in this study: (A) Rhizobium, (B) Ensifer and (C) Shinella.
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pone-0020223-g001: MALDI-TOF MS spectra of whole-cell extracts obtained from the type strains of two close and one divergent species from each genus analysed in this study: (A) Rhizobium, (B) Ensifer and (C) Shinella.

Mentions: Firstly we compared the spectra of the type strains from the type species of the three genera currently included in family Rhizobiaceae. The results obtained showed that the spectra of Rhizobium leguminosarum USDA 2370T, Ensifer adhaerens LMG 20216T and Shinella granuli DSM18401T were clearly distinguishable since there were not common peaks among their spectra (figure 1).


MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.

Ferreira L, Sánchez-Juanes F, García-Fraile P, Rivas R, Mateos PF, Martínez-Molina E, González-Buitrago JM, Velázquez E - PLoS ONE (2011)

MALDI-TOF MS spectra of whole-cell extracts obtained from the type strains of two close and one divergent species from each genus analysed in this study: (A) Rhizobium, (B) Ensifer and (C) Shinella.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105015&req=5

pone-0020223-g001: MALDI-TOF MS spectra of whole-cell extracts obtained from the type strains of two close and one divergent species from each genus analysed in this study: (A) Rhizobium, (B) Ensifer and (C) Shinella.
Mentions: Firstly we compared the spectra of the type strains from the type species of the three genera currently included in family Rhizobiaceae. The results obtained showed that the spectra of Rhizobium leguminosarum USDA 2370T, Ensifer adhaerens LMG 20216T and Shinella granuli DSM18401T were clearly distinguishable since there were not common peaks among their spectra (figure 1).

Bottom Line: Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae.Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences.The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, Spain.

ABSTRACT
Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae. In this work we evaluated the suitability of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for this purpose. Firstly, we evaluated the capability of this methodology to differentiate among species of family Rhizobiaceae including those closely related and then we extended the database of MALDI Biotyper 2.0 including the type strains of 56 species from genera Rhizobium, Ensifer and Shinella. Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences. The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.

Show MeSH