Limits...
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.

Chen H, Sun Y, Dong R, Yang S, Pan C, Xiang D, Miao M, Jiao B - PLoS ONE (2011)

Bottom Line: In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay.A decrease of INHBB and Met was detected in regenerating liver.MiR-34a expression was upregulated during the late phase of liver regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, China.

ABSTRACT

Background: MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.

Methodology/principal finding: MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver.

Conclusion/significance: MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.

Show MeSH

Related in: MedlinePlus

Differentially expressed miRNAs during liver regeneration (LR).(A) miRNA expression profiling at 5 d after partial hepatectomy (PHx). Each data point represents the ratio of miRNA expression levels under PHx to Sham operation (SH). Ratio values >1.5 or <0.7 were considered up-regulated (red) or down-regulated (green) in PHx rats compared to SH rats. (B) The expression pattern of miR-34a during LR by quantitative real-time PCR (qRT-PCR) analysis. miR-34a levels were normalized to that of u6. * P<0.05, ** P<0.01, vs SH control at each time point.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3105003&req=5

pone-0020238-g001: Differentially expressed miRNAs during liver regeneration (LR).(A) miRNA expression profiling at 5 d after partial hepatectomy (PHx). Each data point represents the ratio of miRNA expression levels under PHx to Sham operation (SH). Ratio values >1.5 or <0.7 were considered up-regulated (red) or down-regulated (green) in PHx rats compared to SH rats. (B) The expression pattern of miR-34a during LR by quantitative real-time PCR (qRT-PCR) analysis. miR-34a levels were normalized to that of u6. * P<0.05, ** P<0.01, vs SH control at each time point.

Mentions: To illustrate the miRNA expression pattern in the termination phase of LR, we performed a comprehensive miRNA expression profiling analysis on 6 rats 5 d after PHx or SH. As shown in Figure 1A, we identified 8 up-regulated and 2 down-regulated miRNAs, in which miR-34a had at least 5-fold difference in expression between PHx and SH, with a P value <0.01. We then studied how miR-34a expression changes during LR on different time points. As revealed by qRT-PCR analysis, the regeneration of the remnant livers after PHx caused a transient increase in miR-34a expression with ∼2-fold and ∼6-fold at 3 and 5 d when compared with SH control on each time point (Figure 1B).


Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.

Chen H, Sun Y, Dong R, Yang S, Pan C, Xiang D, Miao M, Jiao B - PLoS ONE (2011)

Differentially expressed miRNAs during liver regeneration (LR).(A) miRNA expression profiling at 5 d after partial hepatectomy (PHx). Each data point represents the ratio of miRNA expression levels under PHx to Sham operation (SH). Ratio values >1.5 or <0.7 were considered up-regulated (red) or down-regulated (green) in PHx rats compared to SH rats. (B) The expression pattern of miR-34a during LR by quantitative real-time PCR (qRT-PCR) analysis. miR-34a levels were normalized to that of u6. * P<0.05, ** P<0.01, vs SH control at each time point.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105003&req=5

pone-0020238-g001: Differentially expressed miRNAs during liver regeneration (LR).(A) miRNA expression profiling at 5 d after partial hepatectomy (PHx). Each data point represents the ratio of miRNA expression levels under PHx to Sham operation (SH). Ratio values >1.5 or <0.7 were considered up-regulated (red) or down-regulated (green) in PHx rats compared to SH rats. (B) The expression pattern of miR-34a during LR by quantitative real-time PCR (qRT-PCR) analysis. miR-34a levels were normalized to that of u6. * P<0.05, ** P<0.01, vs SH control at each time point.
Mentions: To illustrate the miRNA expression pattern in the termination phase of LR, we performed a comprehensive miRNA expression profiling analysis on 6 rats 5 d after PHx or SH. As shown in Figure 1A, we identified 8 up-regulated and 2 down-regulated miRNAs, in which miR-34a had at least 5-fold difference in expression between PHx and SH, with a P value <0.01. We then studied how miR-34a expression changes during LR on different time points. As revealed by qRT-PCR analysis, the regeneration of the remnant livers after PHx caused a transient increase in miR-34a expression with ∼2-fold and ∼6-fold at 3 and 5 d when compared with SH control on each time point (Figure 1B).

Bottom Line: In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay.A decrease of INHBB and Met was detected in regenerating liver.MiR-34a expression was upregulated during the late phase of liver regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, China.

ABSTRACT

Background: MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.

Methodology/principal finding: MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver.

Conclusion/significance: MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.

Show MeSH
Related in: MedlinePlus