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Potential use of a serpin from Arabidopsis for pest control.

Alvarez-Alfageme F, Maharramov J, Carrillo L, Vandenabeele S, Vercammen D, Van Breusegem F, Smagghe G - PLoS ONE (2011)

Bottom Line: Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1.AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls.Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium. fernando.alvarez@art.admin.ch

ABSTRACT
Although genetically modified (GM) plants expressing toxins from Bacillus thuringiensis (Bt) protect agricultural crops against lepidopteran and coleopteran pests, field-evolved resistance to Bt toxins has been reported for populations of several lepidopteran species. Moreover, some important agricultural pests, like phloem-feeding insects, are not susceptible to Bt crops. Complementary pest control strategies are therefore necessary to assure that the benefits provided by those insect-resistant transgenic plants are not compromised and to target those pests that are not susceptible. Experimental GM plants producing plant protease inhibitors have been shown to confer resistance against a wide range of agricultural pests. In this study we assessed the potential of AtSerpin1, a serpin from Arabidopsis thaliana (L). Heynh., for pest control. In vitro assays were conducted with a wide range of pests that rely mainly on either serine or cysteine proteases for digestion and also with three non-target organisms occurring in agricultural crops. AtSerpin1 inhibited proteases from all pest and non-target species assayed. Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1. AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls. Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)). The results indicate that AtSerpin1 is a good candidate for exploitation in pest control.

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Concentration-response curve for mortality of newborn Acyrthosiphon pisum nymphs fed for 3 days with artificial diet containing increasing concentrations of the protease inhibitor AtSerpin1.Points represent mean ± SE. Three to six replicates with 15 nymphs each were used per concentration.
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pone-0020278-g005: Concentration-response curve for mortality of newborn Acyrthosiphon pisum nymphs fed for 3 days with artificial diet containing increasing concentrations of the protease inhibitor AtSerpin1.Points represent mean ± SE. Three to six replicates with 15 nymphs each were used per concentration.

Mentions: A. pisum nymphs reared for 3 days on diets containing 100 to 1000 µg ml−1 AtSerpin1 were highly susceptible to the inhibitor (Figure 5). Mortality reached 77.4% when A. pisum were fed 1000 µg ml−1 of the serpin. The effective AtSerpin1 concentration for 50% mortality (LC50) at the third day of feeding was 637 µg ml−1 (95% confidence limits = 367–1105; R2 = 0.91) (Figure 5). Hence, it appears that AtSerpin1 not only inhibits cysteine proteases in A. pisum extracts in vitro but also has a strong insecticidal effect on nymphs.


Potential use of a serpin from Arabidopsis for pest control.

Alvarez-Alfageme F, Maharramov J, Carrillo L, Vandenabeele S, Vercammen D, Van Breusegem F, Smagghe G - PLoS ONE (2011)

Concentration-response curve for mortality of newborn Acyrthosiphon pisum nymphs fed for 3 days with artificial diet containing increasing concentrations of the protease inhibitor AtSerpin1.Points represent mean ± SE. Three to six replicates with 15 nymphs each were used per concentration.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104999&req=5

pone-0020278-g005: Concentration-response curve for mortality of newborn Acyrthosiphon pisum nymphs fed for 3 days with artificial diet containing increasing concentrations of the protease inhibitor AtSerpin1.Points represent mean ± SE. Three to six replicates with 15 nymphs each were used per concentration.
Mentions: A. pisum nymphs reared for 3 days on diets containing 100 to 1000 µg ml−1 AtSerpin1 were highly susceptible to the inhibitor (Figure 5). Mortality reached 77.4% when A. pisum were fed 1000 µg ml−1 of the serpin. The effective AtSerpin1 concentration for 50% mortality (LC50) at the third day of feeding was 637 µg ml−1 (95% confidence limits = 367–1105; R2 = 0.91) (Figure 5). Hence, it appears that AtSerpin1 not only inhibits cysteine proteases in A. pisum extracts in vitro but also has a strong insecticidal effect on nymphs.

Bottom Line: Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1.AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls.Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium. fernando.alvarez@art.admin.ch

ABSTRACT
Although genetically modified (GM) plants expressing toxins from Bacillus thuringiensis (Bt) protect agricultural crops against lepidopteran and coleopteran pests, field-evolved resistance to Bt toxins has been reported for populations of several lepidopteran species. Moreover, some important agricultural pests, like phloem-feeding insects, are not susceptible to Bt crops. Complementary pest control strategies are therefore necessary to assure that the benefits provided by those insect-resistant transgenic plants are not compromised and to target those pests that are not susceptible. Experimental GM plants producing plant protease inhibitors have been shown to confer resistance against a wide range of agricultural pests. In this study we assessed the potential of AtSerpin1, a serpin from Arabidopsis thaliana (L). Heynh., for pest control. In vitro assays were conducted with a wide range of pests that rely mainly on either serine or cysteine proteases for digestion and also with three non-target organisms occurring in agricultural crops. AtSerpin1 inhibited proteases from all pest and non-target species assayed. Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1. AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls. Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)). The results indicate that AtSerpin1 is a good candidate for exploitation in pest control.

Show MeSH
Related in: MedlinePlus