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Potential use of a serpin from Arabidopsis for pest control.

Alvarez-Alfageme F, Maharramov J, Carrillo L, Vandenabeele S, Vercammen D, Van Breusegem F, Smagghe G - PLoS ONE (2011)

Bottom Line: Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1.AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls.Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium. fernando.alvarez@art.admin.ch

ABSTRACT
Although genetically modified (GM) plants expressing toxins from Bacillus thuringiensis (Bt) protect agricultural crops against lepidopteran and coleopteran pests, field-evolved resistance to Bt toxins has been reported for populations of several lepidopteran species. Moreover, some important agricultural pests, like phloem-feeding insects, are not susceptible to Bt crops. Complementary pest control strategies are therefore necessary to assure that the benefits provided by those insect-resistant transgenic plants are not compromised and to target those pests that are not susceptible. Experimental GM plants producing plant protease inhibitors have been shown to confer resistance against a wide range of agricultural pests. In this study we assessed the potential of AtSerpin1, a serpin from Arabidopsis thaliana (L). Heynh., for pest control. In vitro assays were conducted with a wide range of pests that rely mainly on either serine or cysteine proteases for digestion and also with three non-target organisms occurring in agricultural crops. AtSerpin1 inhibited proteases from all pest and non-target species assayed. Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1. AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls. Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)). The results indicate that AtSerpin1 is a good candidate for exploitation in pest control.

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Weight gain of Spodoptera littoralis larvae fed on a diet containing 65 or 650 µg g−1 AtSerpin1 or control diet without inhibitor.Feeding assays were performed for 6 days with third-instar larvae. Bars represent mean ± SE. Bars with different letters on the same day are significantly different (P<0.05; one-way ANOVA followed by Student-Newman-Keuls) (N = 48).
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pone-0020278-g001: Weight gain of Spodoptera littoralis larvae fed on a diet containing 65 or 650 µg g−1 AtSerpin1 or control diet without inhibitor.Feeding assays were performed for 6 days with third-instar larvae. Bars represent mean ± SE. Bars with different letters on the same day are significantly different (P<0.05; one-way ANOVA followed by Student-Newman-Keuls) (N = 48).

Mentions: Ingestion of artificial diets containing the protease inhibitor AtSerpin1 markedly reduced the weight gain of S. littoralis (Figure 1). A significant difference (P<0.001) occurred after only 2 days of exposure when third instars were reared on artificial diet containing 650 µg g−1 AtSerpin1. This difference continued throughout the bioassay, and on day 6, the weight increase was 65% (P<0.001) lower for S. littoralis larvae ingesting the inhibitor than for the control. For larvae exposed to 65 µg g−1 AtSerpin1, weight gain was significantly reduced by 20% on day 4 and by 33% on day 6 relative to the control (Figure 2).


Potential use of a serpin from Arabidopsis for pest control.

Alvarez-Alfageme F, Maharramov J, Carrillo L, Vandenabeele S, Vercammen D, Van Breusegem F, Smagghe G - PLoS ONE (2011)

Weight gain of Spodoptera littoralis larvae fed on a diet containing 65 or 650 µg g−1 AtSerpin1 or control diet without inhibitor.Feeding assays were performed for 6 days with third-instar larvae. Bars represent mean ± SE. Bars with different letters on the same day are significantly different (P<0.05; one-way ANOVA followed by Student-Newman-Keuls) (N = 48).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104999&req=5

pone-0020278-g001: Weight gain of Spodoptera littoralis larvae fed on a diet containing 65 or 650 µg g−1 AtSerpin1 or control diet without inhibitor.Feeding assays were performed for 6 days with third-instar larvae. Bars represent mean ± SE. Bars with different letters on the same day are significantly different (P<0.05; one-way ANOVA followed by Student-Newman-Keuls) (N = 48).
Mentions: Ingestion of artificial diets containing the protease inhibitor AtSerpin1 markedly reduced the weight gain of S. littoralis (Figure 1). A significant difference (P<0.001) occurred after only 2 days of exposure when third instars were reared on artificial diet containing 650 µg g−1 AtSerpin1. This difference continued throughout the bioassay, and on day 6, the weight increase was 65% (P<0.001) lower for S. littoralis larvae ingesting the inhibitor than for the control. For larvae exposed to 65 µg g−1 AtSerpin1, weight gain was significantly reduced by 20% on day 4 and by 33% on day 6 relative to the control (Figure 2).

Bottom Line: Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1.AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls.Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium. fernando.alvarez@art.admin.ch

ABSTRACT
Although genetically modified (GM) plants expressing toxins from Bacillus thuringiensis (Bt) protect agricultural crops against lepidopteran and coleopteran pests, field-evolved resistance to Bt toxins has been reported for populations of several lepidopteran species. Moreover, some important agricultural pests, like phloem-feeding insects, are not susceptible to Bt crops. Complementary pest control strategies are therefore necessary to assure that the benefits provided by those insect-resistant transgenic plants are not compromised and to target those pests that are not susceptible. Experimental GM plants producing plant protease inhibitors have been shown to confer resistance against a wide range of agricultural pests. In this study we assessed the potential of AtSerpin1, a serpin from Arabidopsis thaliana (L). Heynh., for pest control. In vitro assays were conducted with a wide range of pests that rely mainly on either serine or cysteine proteases for digestion and also with three non-target organisms occurring in agricultural crops. AtSerpin1 inhibited proteases from all pest and non-target species assayed. Subsequently, the cotton leafworm Spodoptera littoralis Boisduval and the pea aphid Acyrthosiphon pisum (Harris) were fed on artificial diets containing AtSerpin1, and S. littoralis was also fed on transgenic Arabidopsis plants overproducing AtSerpin1. AtSerpin1 supplied in the artificial diet or by transgenic plants reduced the growth of S. littoralis larvae by 65% and 38%, respectively, relative to controls. Nymphs of A. pisum exposed to diets containing AtSerpin1 suffered high mortality levels (LC(50) = 637 µg ml(-1)). The results indicate that AtSerpin1 is a good candidate for exploitation in pest control.

Show MeSH
Related in: MedlinePlus