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Loxosceles gaucho venom-induced acute kidney injury--in vivo and in vitro studies.

Lucato RV, Abdulkader RC, Barbaro KC, Mendes GE, Castro I, Baptista MA, Cury PM, Malheiros DM, Schor N, Yu L, Burdmann EA - PLoS Negl Trop Dis (2011)

Bottom Line: There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase.In the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, São José do Rio Preto Medical School, São José do Rio Preto, Brazil.

ABSTRACT

Background: Accidents caused by Loxosceles spider may cause severe systemic reactions, including acute kidney injury (AKI). There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.

Methodology/principal findings: In order to test Loxosceles gaucho venom (LV) nephrotoxicity and to assess some of the possible mechanisms of renal injury, rats were studied up to 60 minutes after LV 0.24 mg/kg or saline IV injection (control). LV caused a sharp and significant drop in glomerular filtration rate, renal blood flow and urinary output and increased renal vascular resistance, without changing blood pressure. Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase. In the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes. Immunohistochemistry disclosed renal deposition of myoglobin and hemoglobin. LV did not cause injury to a suspension of fresh proximal tubules isolated from rats.

Conclusions/significance: Loxosceles gaucho venom injection caused early AKI, which occurred without blood pressure variation. Changes in glomerular function occurred likely due to renal vasoconstriction and rhabdomyolysis. Direct nephrotoxicity could not be demonstrated in vitro. The development of a consistent model of Loxosceles venom-induced AKI and a better understanding of the mechanisms involved in the renal injury may allow more efficient ways to prevent or attenuate the systemic injury after Loxosceles bite.

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Immunohistochemistry for myoglobin and hemoglobin in the renal tissue of control and venom-injected rats.A. Negative staining for myoglobin (200 X). B. Positive tubular cytoplasm staining for myoglobin (black arrows). Note tubular necrosis in the same area (200 X). C. Negative staining for hemoglobin (200 X). D. Positive tubular cytoplasm staining (black arrows) for hemoglobin (200 X).
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pntd-0001182-g001: Immunohistochemistry for myoglobin and hemoglobin in the renal tissue of control and venom-injected rats.A. Negative staining for myoglobin (200 X). B. Positive tubular cytoplasm staining for myoglobin (black arrows). Note tubular necrosis in the same area (200 X). C. Negative staining for hemoglobin (200 X). D. Positive tubular cytoplasm staining (black arrows) for hemoglobin (200 X).

Mentions: These data are represented at Figure 1.


Loxosceles gaucho venom-induced acute kidney injury--in vivo and in vitro studies.

Lucato RV, Abdulkader RC, Barbaro KC, Mendes GE, Castro I, Baptista MA, Cury PM, Malheiros DM, Schor N, Yu L, Burdmann EA - PLoS Negl Trop Dis (2011)

Immunohistochemistry for myoglobin and hemoglobin in the renal tissue of control and venom-injected rats.A. Negative staining for myoglobin (200 X). B. Positive tubular cytoplasm staining for myoglobin (black arrows). Note tubular necrosis in the same area (200 X). C. Negative staining for hemoglobin (200 X). D. Positive tubular cytoplasm staining (black arrows) for hemoglobin (200 X).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104973&req=5

pntd-0001182-g001: Immunohistochemistry for myoglobin and hemoglobin in the renal tissue of control and venom-injected rats.A. Negative staining for myoglobin (200 X). B. Positive tubular cytoplasm staining for myoglobin (black arrows). Note tubular necrosis in the same area (200 X). C. Negative staining for hemoglobin (200 X). D. Positive tubular cytoplasm staining (black arrows) for hemoglobin (200 X).
Mentions: These data are represented at Figure 1.

Bottom Line: There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase.In the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes.

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, São José do Rio Preto Medical School, São José do Rio Preto, Brazil.

ABSTRACT

Background: Accidents caused by Loxosceles spider may cause severe systemic reactions, including acute kidney injury (AKI). There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.

Methodology/principal findings: In order to test Loxosceles gaucho venom (LV) nephrotoxicity and to assess some of the possible mechanisms of renal injury, rats were studied up to 60 minutes after LV 0.24 mg/kg or saline IV injection (control). LV caused a sharp and significant drop in glomerular filtration rate, renal blood flow and urinary output and increased renal vascular resistance, without changing blood pressure. Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase. In the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes. Immunohistochemistry disclosed renal deposition of myoglobin and hemoglobin. LV did not cause injury to a suspension of fresh proximal tubules isolated from rats.

Conclusions/significance: Loxosceles gaucho venom injection caused early AKI, which occurred without blood pressure variation. Changes in glomerular function occurred likely due to renal vasoconstriction and rhabdomyolysis. Direct nephrotoxicity could not be demonstrated in vitro. The development of a consistent model of Loxosceles venom-induced AKI and a better understanding of the mechanisms involved in the renal injury may allow more efficient ways to prevent or attenuate the systemic injury after Loxosceles bite.

Show MeSH
Related in: MedlinePlus