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Vector competence of the tick Ixodes ricinus for transmission of Bartonella birtlesii.

Reis C, Cote M, Le Rhun D, Lecuelle B, Levin ML, Vayssier-Taussat M, Bonnet SI - PLoS Negl Trop Dis (2011)

Bottom Line: We used a murine model to assess the vector competence of Ixodes ricinus for Bartonella birtlesii.Larval and nymphal I. ricinus were fed on a B. birtlesii-infected mouse.Consequently, bartonelloses should be now included in the differential diagnosis for patients exposed to tick bites.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, USC INRA Bartonella-Tiques, ANSES, Maisons-Alfort, France.

ABSTRACT
Bartonella spp. are facultative intracellular vector-borne bacteria associated with several emerging diseases in humans and animals all over the world. The potential for involvement of ticks in transmission of Bartonella spp. has been heartily debated for many years. However, most of the data supporting bartonellae transmission by ticks come from molecular and serological epidemiological surveys in humans and animals providing only indirect evidences without a direct proof of tick vector competence for transmission of bartonellae. We used a murine model to assess the vector competence of Ixodes ricinus for Bartonella birtlesii. Larval and nymphal I. ricinus were fed on a B. birtlesii-infected mouse. The nymphs successfully transmitted B. birtlesii to naïve mice as bacteria were recovered from both the mouse blood and liver at seven and 16 days after tick bites. The female adults successfully emitted the bacteria into uninfected blood after three or more days of tick attachment, when fed via membrane feeding system. Histochemical staining showed the presence of bacteria in salivary glands and muscle tissues of partially engorged adult ticks, which had molted from the infected nymphs. These results confirm the vector competence of I. ricinus for B. birtlesii and represent the first in vivo demonstration of a Bartonella sp. transmission by ticks. Consequently, bartonelloses should be now included in the differential diagnosis for patients exposed to tick bites.

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Blood contamination by female ticks.Detection of Bartonella DNA by semi-nested PCR in the blood from a feeder after placement of B. birtlesii-infected adult I. ricinus on the membrane: Lines D0–D8 represent blood samples taken on days 0 through 8 after tick placement; M – molecular mass marker.; T− and T+ – negative (distilled water) and positive controls (B. birtlesii DNA).
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pntd-0001186-g002: Blood contamination by female ticks.Detection of Bartonella DNA by semi-nested PCR in the blood from a feeder after placement of B. birtlesii-infected adult I. ricinus on the membrane: Lines D0–D8 represent blood samples taken on days 0 through 8 after tick placement; M – molecular mass marker.; T− and T+ – negative (distilled water) and positive controls (B. birtlesii DNA).

Mentions: Thirteen female I. ricinus fed at the preceding nymphal life stage upon a B. birtlesii–infected mouse were re-fed with uninfected sheep blood on a membrane feeder. Blood samples were withdrawn from the feeder every 24 h during the 8–day feeding period to detect the presence of B. birtlesii DNA. B. birtlesii DNA was detected in samples drawn on days three through eight of tick attachment (Figure 2), indicating that adult ticks were successfully emitting the bacteria into the previously uninfected blood during feeding.


Vector competence of the tick Ixodes ricinus for transmission of Bartonella birtlesii.

Reis C, Cote M, Le Rhun D, Lecuelle B, Levin ML, Vayssier-Taussat M, Bonnet SI - PLoS Negl Trop Dis (2011)

Blood contamination by female ticks.Detection of Bartonella DNA by semi-nested PCR in the blood from a feeder after placement of B. birtlesii-infected adult I. ricinus on the membrane: Lines D0–D8 represent blood samples taken on days 0 through 8 after tick placement; M – molecular mass marker.; T− and T+ – negative (distilled water) and positive controls (B. birtlesii DNA).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104967&req=5

pntd-0001186-g002: Blood contamination by female ticks.Detection of Bartonella DNA by semi-nested PCR in the blood from a feeder after placement of B. birtlesii-infected adult I. ricinus on the membrane: Lines D0–D8 represent blood samples taken on days 0 through 8 after tick placement; M – molecular mass marker.; T− and T+ – negative (distilled water) and positive controls (B. birtlesii DNA).
Mentions: Thirteen female I. ricinus fed at the preceding nymphal life stage upon a B. birtlesii–infected mouse were re-fed with uninfected sheep blood on a membrane feeder. Blood samples were withdrawn from the feeder every 24 h during the 8–day feeding period to detect the presence of B. birtlesii DNA. B. birtlesii DNA was detected in samples drawn on days three through eight of tick attachment (Figure 2), indicating that adult ticks were successfully emitting the bacteria into the previously uninfected blood during feeding.

Bottom Line: We used a murine model to assess the vector competence of Ixodes ricinus for Bartonella birtlesii.Larval and nymphal I. ricinus were fed on a B. birtlesii-infected mouse.Consequently, bartonelloses should be now included in the differential diagnosis for patients exposed to tick bites.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, USC INRA Bartonella-Tiques, ANSES, Maisons-Alfort, France.

ABSTRACT
Bartonella spp. are facultative intracellular vector-borne bacteria associated with several emerging diseases in humans and animals all over the world. The potential for involvement of ticks in transmission of Bartonella spp. has been heartily debated for many years. However, most of the data supporting bartonellae transmission by ticks come from molecular and serological epidemiological surveys in humans and animals providing only indirect evidences without a direct proof of tick vector competence for transmission of bartonellae. We used a murine model to assess the vector competence of Ixodes ricinus for Bartonella birtlesii. Larval and nymphal I. ricinus were fed on a B. birtlesii-infected mouse. The nymphs successfully transmitted B. birtlesii to naïve mice as bacteria were recovered from both the mouse blood and liver at seven and 16 days after tick bites. The female adults successfully emitted the bacteria into uninfected blood after three or more days of tick attachment, when fed via membrane feeding system. Histochemical staining showed the presence of bacteria in salivary glands and muscle tissues of partially engorged adult ticks, which had molted from the infected nymphs. These results confirm the vector competence of I. ricinus for B. birtlesii and represent the first in vivo demonstration of a Bartonella sp. transmission by ticks. Consequently, bartonelloses should be now included in the differential diagnosis for patients exposed to tick bites.

Show MeSH
Related in: MedlinePlus