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Exercise training prevents endometrial hyperplasia and biomarkers for endometrial cancer in rat model of type 1 diabetes.

Al-Jarrah M, Matalka I, Aseri HA, Mohtaseb A, Smirnova IV, Novikova L, Stehno-Bittel L, Alkhateeb A - J Clin Med Res (2010)

Bottom Line: A 4-week treadmill training program was initiated with the development of diabetes.The expression of ERα increased significantly (p < 0.02) while the expression level of p16 decreased significantly (p < 0.04) in the diabetic sedentary group compared to the non-diabetic groups.Exercise training led to a reversal in the percentage of p16 and ERα positive cells in diabetic rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Allied Medical Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Jordan.

ABSTRACT

Background: Endometrial cancer is one of the most common types of gynecologic cancers. The ability of exercise to reduce the risk of endometrial cancer in women with type 2 diabetes has been established, but no studies have examined this link in type 1 diabetes.A randomized, controlled animal study was designed using a standard rat model of type 1 diabetes. The goal of this study was to investigate the ability of exercise to prevent increased levels of endometrial cancer biomarkers, estrogen receptor (ERα) and p16, and endometrial hyperplasia associated with diabetes.

Methods: FORTY FEMALE RATS WERE RANDOMIZED INTO FOUR GROUPS: sedentary control, exercise control, sedentary or exercised diabetic. Diabetes was induced by alloxan injection. A 4-week treadmill training program was initiated with the development of diabetes. Endometrial tissues were evaluated for hyperplasia and ERα and p16 levels and subcellular localization using microscopy.

Results: Severe diabetes lead to hyperplasia in the endometrial tissue in 70% of sedentary diabetic rats. Exercise-trained diabetic rats and the non-diabetic rats displayed no hyperplasia. The expression of ERα increased significantly (p < 0.02) while the expression level of p16 decreased significantly (p < 0.04) in the diabetic sedentary group compared to the non-diabetic groups. Exercise training led to a reversal in the percentage of p16 and ERα positive cells in diabetic rats.

Conclusions: Severe diabetes leads to hyperplasia of the endometrial tissue and increased ERα levels and decreased p16 levels in rats, which can be prevented with aerobic exercise.

Keywords: Diabetes; Estrogen receptor alpha; P16; Endometrial hyperplasia; Endometrial cancer; Exercise.

No MeSH data available.


Related in: MedlinePlus

Histological analysis of endometrial tissue. A) Typical section from a sedentary control rat. B) Typical section from a sedentary diabetic rat showing excessive glands (arrows) with tubular cells surrounding the glands, along with signs of hyperplasia.
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Figure 4: Histological analysis of endometrial tissue. A) Typical section from a sedentary control rat. B) Typical section from a sedentary diabetic rat showing excessive glands (arrows) with tubular cells surrounding the glands, along with signs of hyperplasia.

Mentions: Seventy percent of the specimens analyzed from the sedentary diabetic group showed elements of simple glandular hyperplasia including excessive endometrial glands. No hyperplasia was detected in the control groups or the exercised diabetic group. Figure 4 provides examples of stained specimen from the two diabetic groups (sedentary and exercised). The images show excessive glands and tubular cells with large nuclei typical of simple hyperplasia in sections from the sedentary diabetic animals. Endometrium from the non-diabetic groups was comprised of healthy spindle-shaped cells. The sedentary control and exercise-trained diabetic groups had means of 0.52 ± 0.14 and 0.98 ± 0.26 glands per region of interest, respectively (Figure 5). Tissues obtained from the sedentary diabetic group had an average of 5.21 ± 0.75 glands, signifying nearly ten times more glands per area when compared to the other groups (p < 0.001). None of the sections examined contained tumors.


Exercise training prevents endometrial hyperplasia and biomarkers for endometrial cancer in rat model of type 1 diabetes.

Al-Jarrah M, Matalka I, Aseri HA, Mohtaseb A, Smirnova IV, Novikova L, Stehno-Bittel L, Alkhateeb A - J Clin Med Res (2010)

Histological analysis of endometrial tissue. A) Typical section from a sedentary control rat. B) Typical section from a sedentary diabetic rat showing excessive glands (arrows) with tubular cells surrounding the glands, along with signs of hyperplasia.
© Copyright Policy - open access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104659&req=5

Figure 4: Histological analysis of endometrial tissue. A) Typical section from a sedentary control rat. B) Typical section from a sedentary diabetic rat showing excessive glands (arrows) with tubular cells surrounding the glands, along with signs of hyperplasia.
Mentions: Seventy percent of the specimens analyzed from the sedentary diabetic group showed elements of simple glandular hyperplasia including excessive endometrial glands. No hyperplasia was detected in the control groups or the exercised diabetic group. Figure 4 provides examples of stained specimen from the two diabetic groups (sedentary and exercised). The images show excessive glands and tubular cells with large nuclei typical of simple hyperplasia in sections from the sedentary diabetic animals. Endometrium from the non-diabetic groups was comprised of healthy spindle-shaped cells. The sedentary control and exercise-trained diabetic groups had means of 0.52 ± 0.14 and 0.98 ± 0.26 glands per region of interest, respectively (Figure 5). Tissues obtained from the sedentary diabetic group had an average of 5.21 ± 0.75 glands, signifying nearly ten times more glands per area when compared to the other groups (p < 0.001). None of the sections examined contained tumors.

Bottom Line: A 4-week treadmill training program was initiated with the development of diabetes.The expression of ERα increased significantly (p < 0.02) while the expression level of p16 decreased significantly (p < 0.04) in the diabetic sedentary group compared to the non-diabetic groups.Exercise training led to a reversal in the percentage of p16 and ERα positive cells in diabetic rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Allied Medical Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Jordan.

ABSTRACT

Background: Endometrial cancer is one of the most common types of gynecologic cancers. The ability of exercise to reduce the risk of endometrial cancer in women with type 2 diabetes has been established, but no studies have examined this link in type 1 diabetes.A randomized, controlled animal study was designed using a standard rat model of type 1 diabetes. The goal of this study was to investigate the ability of exercise to prevent increased levels of endometrial cancer biomarkers, estrogen receptor (ERα) and p16, and endometrial hyperplasia associated with diabetes.

Methods: FORTY FEMALE RATS WERE RANDOMIZED INTO FOUR GROUPS: sedentary control, exercise control, sedentary or exercised diabetic. Diabetes was induced by alloxan injection. A 4-week treadmill training program was initiated with the development of diabetes. Endometrial tissues were evaluated for hyperplasia and ERα and p16 levels and subcellular localization using microscopy.

Results: Severe diabetes lead to hyperplasia in the endometrial tissue in 70% of sedentary diabetic rats. Exercise-trained diabetic rats and the non-diabetic rats displayed no hyperplasia. The expression of ERα increased significantly (p < 0.02) while the expression level of p16 decreased significantly (p < 0.04) in the diabetic sedentary group compared to the non-diabetic groups. Exercise training led to a reversal in the percentage of p16 and ERα positive cells in diabetic rats.

Conclusions: Severe diabetes leads to hyperplasia of the endometrial tissue and increased ERα levels and decreased p16 levels in rats, which can be prevented with aerobic exercise.

Keywords: Diabetes; Estrogen receptor alpha; P16; Endometrial hyperplasia; Endometrial cancer; Exercise.

No MeSH data available.


Related in: MedlinePlus