Limits...
Expression of vascular endothelial growth factor on chondrocytes increases with osteoarthritis - an animal experimental investigation.

Tibesku CO, Daniilidis K, Skwara A, Paletta J, Szuwart T, Fuchs-Winkelmann S - Open Orthop J (2011)

Bottom Line: There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks.A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).An increase of VEGF expressing chondrocytes occurs during time course of OA.

View Article: PubMed Central - PubMed

Affiliation: Sporthopaedicum Straubing, Germany.

ABSTRACT

Objective: To evaluate the expression of VEGF by chondrocytes of hyaline cartilage during the course of osteoarthritis (OA).

Methods: In 12 white New Zealand rabbits the anterior cruciate ligament (ACL) was resected to create an anterior instability of the knee. In 12 control rabbits only a sham operation without resection of the ACL was done. Four animals of each group were killed at 3, 6, and 12 weeks. The load bearing area was evaluated histologically according to Mankin and by immunostaining for VEGF.

Results: In the experimental group, histological grades of OA showed a positive linear correlation with the time after surgery. Immunostaining showed an increased expression of VEGF in the control group after 3 weeks, which dropped to normal after 6 weeks. There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks. A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).

Conclusions: An increase of VEGF expressing chondrocytes occurs during time course of OA.

No MeSH data available.


Related in: MedlinePlus

12 weeks after acl resection showing pronounced osteophytes and exposure of the subchondral cartilage zone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3104593&req=5

Figure 6: 12 weeks after acl resection showing pronounced osteophytes and exposure of the subchondral cartilage zone.

Mentions: According to the Mankin score, the experimental group showed a highly significant increase in the grade of OA in comparison with the control group and the untreated group (Figs.3-6). In the untreated group the mean (SD) grade of OA was 1.75 (1.58). In the control group it was 3.25 (0.71) after 3 weeks, 1 (1.20) after 6 weeks, and 2.25 (1.17) after 12 weeks. In the experimental group, the scores were 7.25(3.62) after 3 weeks, 8 (2.88) after 6 weeks, and 9.83 (3.06) after 12 weeks. The experimental group had significantly higher values than the control group at every point of time (p<0.01). The average histological grade of OA increased with the number of postoperative weeks. It is remarkable that in the control group the histological grading increases within the first three weeks and drops to normal in the following time.


Expression of vascular endothelial growth factor on chondrocytes increases with osteoarthritis - an animal experimental investigation.

Tibesku CO, Daniilidis K, Skwara A, Paletta J, Szuwart T, Fuchs-Winkelmann S - Open Orthop J (2011)

12 weeks after acl resection showing pronounced osteophytes and exposure of the subchondral cartilage zone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104593&req=5

Figure 6: 12 weeks after acl resection showing pronounced osteophytes and exposure of the subchondral cartilage zone.
Mentions: According to the Mankin score, the experimental group showed a highly significant increase in the grade of OA in comparison with the control group and the untreated group (Figs.3-6). In the untreated group the mean (SD) grade of OA was 1.75 (1.58). In the control group it was 3.25 (0.71) after 3 weeks, 1 (1.20) after 6 weeks, and 2.25 (1.17) after 12 weeks. In the experimental group, the scores were 7.25(3.62) after 3 weeks, 8 (2.88) after 6 weeks, and 9.83 (3.06) after 12 weeks. The experimental group had significantly higher values than the control group at every point of time (p<0.01). The average histological grade of OA increased with the number of postoperative weeks. It is remarkable that in the control group the histological grading increases within the first three weeks and drops to normal in the following time.

Bottom Line: There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks.A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).An increase of VEGF expressing chondrocytes occurs during time course of OA.

View Article: PubMed Central - PubMed

Affiliation: Sporthopaedicum Straubing, Germany.

ABSTRACT

Objective: To evaluate the expression of VEGF by chondrocytes of hyaline cartilage during the course of osteoarthritis (OA).

Methods: In 12 white New Zealand rabbits the anterior cruciate ligament (ACL) was resected to create an anterior instability of the knee. In 12 control rabbits only a sham operation without resection of the ACL was done. Four animals of each group were killed at 3, 6, and 12 weeks. The load bearing area was evaluated histologically according to Mankin and by immunostaining for VEGF.

Results: In the experimental group, histological grades of OA showed a positive linear correlation with the time after surgery. Immunostaining showed an increased expression of VEGF in the control group after 3 weeks, which dropped to normal after 6 weeks. There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks. A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).

Conclusions: An increase of VEGF expressing chondrocytes occurs during time course of OA.

No MeSH data available.


Related in: MedlinePlus