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Expression of vascular endothelial growth factor on chondrocytes increases with osteoarthritis - an animal experimental investigation.

Tibesku CO, Daniilidis K, Skwara A, Paletta J, Szuwart T, Fuchs-Winkelmann S - Open Orthop J (2011)

Bottom Line: There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks.A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).An increase of VEGF expressing chondrocytes occurs during time course of OA.

View Article: PubMed Central - PubMed

Affiliation: Sporthopaedicum Straubing, Germany.

ABSTRACT

Objective: To evaluate the expression of VEGF by chondrocytes of hyaline cartilage during the course of osteoarthritis (OA).

Methods: In 12 white New Zealand rabbits the anterior cruciate ligament (ACL) was resected to create an anterior instability of the knee. In 12 control rabbits only a sham operation without resection of the ACL was done. Four animals of each group were killed at 3, 6, and 12 weeks. The load bearing area was evaluated histologically according to Mankin and by immunostaining for VEGF.

Results: In the experimental group, histological grades of OA showed a positive linear correlation with the time after surgery. Immunostaining showed an increased expression of VEGF in the control group after 3 weeks, which dropped to normal after 6 weeks. There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks. A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).

Conclusions: An increase of VEGF expressing chondrocytes occurs during time course of OA.

No MeSH data available.


Related in: MedlinePlus

Time course of the development of VEGF expression in osteoarthritis (light bar presents the sham operation, grey bar the acl resection group). The percentage of VEGF expressing chondrocytes was significantly increased in the ACL resection group after 6 (p=0.01) and 12 (p=0.05) weeks. Data represent mean ± SD from 4 independent determinations.
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Figure 1: Time course of the development of VEGF expression in osteoarthritis (light bar presents the sham operation, grey bar the acl resection group). The percentage of VEGF expressing chondrocytes was significantly increased in the ACL resection group after 6 (p=0.01) and 12 (p=0.05) weeks. Data represent mean ± SD from 4 independent determinations.

Mentions: Chondrocytes expressing VEGF were found in the experimental and control groups (Fig. 1). In the control group, the mean percentage (SD) of VEGF positive cells was 16.2 ± 4.7 % after 3 weeks, 11.5 ± 5.9 % after 6 weeks, and 11.6 ± 3.3 % after 12 weeks. In the experimental group, percentages were 17.7 ± 5.7 % after 3 weeks,19.4 ± 4.6 % after 6 weeks, and 21.3 ± 5.4 % after 12 weeks (Fig.1).There was no significant difference between control and experimental groups after 3 weeks (p= 0.594), but a significant difference was seen after 6 and 12 weeks (p=0.01 and p=0.05). It is remarkable that in the control group the percentage of VEGF expressing chondrocytes increases within the fist three weeks and drops to normal in the following time.


Expression of vascular endothelial growth factor on chondrocytes increases with osteoarthritis - an animal experimental investigation.

Tibesku CO, Daniilidis K, Skwara A, Paletta J, Szuwart T, Fuchs-Winkelmann S - Open Orthop J (2011)

Time course of the development of VEGF expression in osteoarthritis (light bar presents the sham operation, grey bar the acl resection group). The percentage of VEGF expressing chondrocytes was significantly increased in the ACL resection group after 6 (p=0.01) and 12 (p=0.05) weeks. Data represent mean ± SD from 4 independent determinations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104593&req=5

Figure 1: Time course of the development of VEGF expression in osteoarthritis (light bar presents the sham operation, grey bar the acl resection group). The percentage of VEGF expressing chondrocytes was significantly increased in the ACL resection group after 6 (p=0.01) and 12 (p=0.05) weeks. Data represent mean ± SD from 4 independent determinations.
Mentions: Chondrocytes expressing VEGF were found in the experimental and control groups (Fig. 1). In the control group, the mean percentage (SD) of VEGF positive cells was 16.2 ± 4.7 % after 3 weeks, 11.5 ± 5.9 % after 6 weeks, and 11.6 ± 3.3 % after 12 weeks. In the experimental group, percentages were 17.7 ± 5.7 % after 3 weeks,19.4 ± 4.6 % after 6 weeks, and 21.3 ± 5.4 % after 12 weeks (Fig.1).There was no significant difference between control and experimental groups after 3 weeks (p= 0.594), but a significant difference was seen after 6 and 12 weeks (p=0.01 and p=0.05). It is remarkable that in the control group the percentage of VEGF expressing chondrocytes increases within the fist three weeks and drops to normal in the following time.

Bottom Line: There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks.A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).An increase of VEGF expressing chondrocytes occurs during time course of OA.

View Article: PubMed Central - PubMed

Affiliation: Sporthopaedicum Straubing, Germany.

ABSTRACT

Objective: To evaluate the expression of VEGF by chondrocytes of hyaline cartilage during the course of osteoarthritis (OA).

Methods: In 12 white New Zealand rabbits the anterior cruciate ligament (ACL) was resected to create an anterior instability of the knee. In 12 control rabbits only a sham operation without resection of the ACL was done. Four animals of each group were killed at 3, 6, and 12 weeks. The load bearing area was evaluated histologically according to Mankin and by immunostaining for VEGF.

Results: In the experimental group, histological grades of OA showed a positive linear correlation with the time after surgery. Immunostaining showed an increased expression of VEGF in the control group after 3 weeks, which dropped to normal after 6 weeks. There was no difference in the progression of OA between control and experimental groups after 3 weeks, but a significant difference was seen after 6 (p=0,01) and 12 (p=0,05) weeks. A significant positive correlation between VEGF expression and the histological grade of OA was found (r = 0.767; p<0.01).

Conclusions: An increase of VEGF expressing chondrocytes occurs during time course of OA.

No MeSH data available.


Related in: MedlinePlus