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Apoptosis of T lymphocytes isolated from peripheral blood of patients with acute exacerbation of chronic obstructive pulmonary disease.

Lim SC, Ju JY, Chi SY, Ban HJ, Kwon YS, Oh IJ, Kim KS, Kim YI, Kim YC - Yonsei Med. J. (2011)

Bottom Line: Apoptosis is critical for the maintenance of normal tissue homeostasis and is in equilibrium with proliferation and differentiation.Serum levels of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α were determined by an immunoassay technique.Serum levels of IL-6, IL-8, and TNF-α were significantly increased in patients with exacerbation of COPD compared with stable COPD.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Chonnam National University Hospital, 671 Jaebong-ro, Dong-gu, Gwangju 501-757, Korea. lscmd@jnu.ac.kr

ABSTRACT

Purpose: Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation of the airways and progressive destruction of lung parenchyma. Apoptosis is critical for the maintenance of normal tissue homeostasis and is in equilibrium with proliferation and differentiation. This study was undertaken to investigate relationship between apoptosis of peripheral blood lymphocytes during exacerbation of COPD and inflammatory response that characterizes this condition.

Materials and methods: Seventeen patients with COPD exacerbation, 21 stable COPD, and 12 control subjects were included. T lymphocytes were isolated from peripheral blood using MACS. Apoptosis of T lymphocytes was assessed with FACS using annexin V and 7-aminoactinomycin. Serum levels of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α were determined by an immunoassay technique.

Results: There was significantly increased percentage of apoptotic lymphocytes, CD 4+, and CD 8+ T cells in the peripheral blood of patients with exacerbation of COPD compared with stable COPD. Serum levels of IL-6, IL-8, and TNF-α were significantly increased in patients with exacerbation of COPD compared with stable COPD. Only TNF-α presented a positive correlation with apoptotic lymphocytes in patients with exacerbation of COPD.

Conclusion: Increased apoptotic lymphocytes may be associated with upregulation of TNF-α in the peripheral blood of patients with acute exacerbation of COPD.

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Related in: MedlinePlus

The serum levels of IL-6 (A), IL-8 (B), and TNF-α (C) in control groups, stable COPD, and exacerbation of COPD. Serum cytokine levels are expressed as the median value and interquartile range. •, outlier extending >1.5 box-lengths from the edge of the box. IL-6, interleukin-6; IL-8, interleukin-8; TNF-α, tumor necrosis factor-alpha. COPD, chronic obstructive pulmonary disease.
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Figure 2: The serum levels of IL-6 (A), IL-8 (B), and TNF-α (C) in control groups, stable COPD, and exacerbation of COPD. Serum cytokine levels are expressed as the median value and interquartile range. •, outlier extending >1.5 box-lengths from the edge of the box. IL-6, interleukin-6; IL-8, interleukin-8; TNF-α, tumor necrosis factor-alpha. COPD, chronic obstructive pulmonary disease.

Mentions: Serum levels of IL-6 were significantly increased in patients with exacerbation of COPD compared with the stable COPD and control groups [median, IQR, expressed in pg/mL: exacerbation COPD 19.67 (13.55-23.25); stable COPD 6.58 (3.19-9.54); control group 2.86 (2.35-4.62), ANOVA p<0.001] (Fig. 2A). Serum IL-8 levels were significantly higher in patients with exacerbation of COPD than in the stable COPD and control groups [median, IQR, expressed in pg/ml: exacerbation COPD 87.87 (70.31-91.51); stable COPD 30.38 (27.46-39.83); control group 27.36 (26.08-32.20), ANOVA p<0.001] (Fig. 2B). However, no differences were observed in IL-6 and IL-8 levels between the stable COPD and control groups. Serum levels of TNF-α were significantly increased in patients with exacerbation of COPD compared with stable COPD and control groups. Also increased levels of TNF-α were observed in stable COPD patients compared with control groups [median, IQR, expressed in pg/mL: exacerbation COPD 139.33 (105.98-175.17); stable COPD 57.31 (29.38-109.03); control group 26.05 (21.67-29.07), ANOVA p<0.001] (Fig. 2C).


Apoptosis of T lymphocytes isolated from peripheral blood of patients with acute exacerbation of chronic obstructive pulmonary disease.

Lim SC, Ju JY, Chi SY, Ban HJ, Kwon YS, Oh IJ, Kim KS, Kim YI, Kim YC - Yonsei Med. J. (2011)

The serum levels of IL-6 (A), IL-8 (B), and TNF-α (C) in control groups, stable COPD, and exacerbation of COPD. Serum cytokine levels are expressed as the median value and interquartile range. •, outlier extending >1.5 box-lengths from the edge of the box. IL-6, interleukin-6; IL-8, interleukin-8; TNF-α, tumor necrosis factor-alpha. COPD, chronic obstructive pulmonary disease.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104462&req=5

Figure 2: The serum levels of IL-6 (A), IL-8 (B), and TNF-α (C) in control groups, stable COPD, and exacerbation of COPD. Serum cytokine levels are expressed as the median value and interquartile range. •, outlier extending >1.5 box-lengths from the edge of the box. IL-6, interleukin-6; IL-8, interleukin-8; TNF-α, tumor necrosis factor-alpha. COPD, chronic obstructive pulmonary disease.
Mentions: Serum levels of IL-6 were significantly increased in patients with exacerbation of COPD compared with the stable COPD and control groups [median, IQR, expressed in pg/mL: exacerbation COPD 19.67 (13.55-23.25); stable COPD 6.58 (3.19-9.54); control group 2.86 (2.35-4.62), ANOVA p<0.001] (Fig. 2A). Serum IL-8 levels were significantly higher in patients with exacerbation of COPD than in the stable COPD and control groups [median, IQR, expressed in pg/ml: exacerbation COPD 87.87 (70.31-91.51); stable COPD 30.38 (27.46-39.83); control group 27.36 (26.08-32.20), ANOVA p<0.001] (Fig. 2B). However, no differences were observed in IL-6 and IL-8 levels between the stable COPD and control groups. Serum levels of TNF-α were significantly increased in patients with exacerbation of COPD compared with stable COPD and control groups. Also increased levels of TNF-α were observed in stable COPD patients compared with control groups [median, IQR, expressed in pg/mL: exacerbation COPD 139.33 (105.98-175.17); stable COPD 57.31 (29.38-109.03); control group 26.05 (21.67-29.07), ANOVA p<0.001] (Fig. 2C).

Bottom Line: Apoptosis is critical for the maintenance of normal tissue homeostasis and is in equilibrium with proliferation and differentiation.Serum levels of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α were determined by an immunoassay technique.Serum levels of IL-6, IL-8, and TNF-α were significantly increased in patients with exacerbation of COPD compared with stable COPD.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Chonnam National University Hospital, 671 Jaebong-ro, Dong-gu, Gwangju 501-757, Korea. lscmd@jnu.ac.kr

ABSTRACT

Purpose: Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation of the airways and progressive destruction of lung parenchyma. Apoptosis is critical for the maintenance of normal tissue homeostasis and is in equilibrium with proliferation and differentiation. This study was undertaken to investigate relationship between apoptosis of peripheral blood lymphocytes during exacerbation of COPD and inflammatory response that characterizes this condition.

Materials and methods: Seventeen patients with COPD exacerbation, 21 stable COPD, and 12 control subjects were included. T lymphocytes were isolated from peripheral blood using MACS. Apoptosis of T lymphocytes was assessed with FACS using annexin V and 7-aminoactinomycin. Serum levels of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α were determined by an immunoassay technique.

Results: There was significantly increased percentage of apoptotic lymphocytes, CD 4+, and CD 8+ T cells in the peripheral blood of patients with exacerbation of COPD compared with stable COPD. Serum levels of IL-6, IL-8, and TNF-α were significantly increased in patients with exacerbation of COPD compared with stable COPD. Only TNF-α presented a positive correlation with apoptotic lymphocytes in patients with exacerbation of COPD.

Conclusion: Increased apoptotic lymphocytes may be associated with upregulation of TNF-α in the peripheral blood of patients with acute exacerbation of COPD.

Show MeSH
Related in: MedlinePlus