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Lack of effect of dexamethasone on growth of Orientia tsutsugamushi Gilliam in mouse L929 cells.

Kim CO, Huh AJ, Yeom JS, Lee KS, Chin BS, Han SH, Jeong SJ, Choi JY, Song YG, Kim JM - Yonsei Med. J. (2011)

Bottom Line: Treatment of the cells with dexamethasone at concentrations of 10¹ and 10⁷ pg/mL showed no influence on the growth of Orientia tsutsugamushi.Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro.Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Yonsei University College of Medicine, 250 Seongsan-ro, Seodaemun-gu, Seoul 120-752, Korea.

ABSTRACT

Purpose: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease.

Materials and methods: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 10¹ and 10⁷ pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone.

Results: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fluorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 10¹ and 10⁷ pg/mL showed no influence on the growth of Orientia tsutsugamushi.

Conclusion: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

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Effect of IFN-γ on the growth of Orientia tsutsugamushi. To evaluate the stability of our results, experiments that assessed the effect of interferon-γ on the growth of Orientia tsutsugamushi by flow cytometry were used as the control group.
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Figure 4: Effect of IFN-γ on the growth of Orientia tsutsugamushi. To evaluate the stability of our results, experiments that assessed the effect of interferon-γ on the growth of Orientia tsutsugamushi by flow cytometry were used as the control group.

Mentions: Corticosteroid concentration was raised from 101 pg/mL to 107 pg/mL by 10-fold. The growth level of Orientia tsutsugaobmushi was evaluated by flow cytometry and the results showed that differences in the growth of Orientia tsutsugamushi with or without the addition of corticosteroids as well as added dosages were not detected (Fig. 3). To evaluate the reliability of our results, experiments that assessed the effects of interferon on the growth of Orientia tsutsugamushi by flow cytometry reported in other studies were used as the control group in our study and, similar to the previously reported results, reduction of the growth of Orientia tsutsugamushi in response to interferon-γ administration was confirmed (Fig. 4).8


Lack of effect of dexamethasone on growth of Orientia tsutsugamushi Gilliam in mouse L929 cells.

Kim CO, Huh AJ, Yeom JS, Lee KS, Chin BS, Han SH, Jeong SJ, Choi JY, Song YG, Kim JM - Yonsei Med. J. (2011)

Effect of IFN-γ on the growth of Orientia tsutsugamushi. To evaluate the stability of our results, experiments that assessed the effect of interferon-γ on the growth of Orientia tsutsugamushi by flow cytometry were used as the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104446&req=5

Figure 4: Effect of IFN-γ on the growth of Orientia tsutsugamushi. To evaluate the stability of our results, experiments that assessed the effect of interferon-γ on the growth of Orientia tsutsugamushi by flow cytometry were used as the control group.
Mentions: Corticosteroid concentration was raised from 101 pg/mL to 107 pg/mL by 10-fold. The growth level of Orientia tsutsugaobmushi was evaluated by flow cytometry and the results showed that differences in the growth of Orientia tsutsugamushi with or without the addition of corticosteroids as well as added dosages were not detected (Fig. 3). To evaluate the reliability of our results, experiments that assessed the effects of interferon on the growth of Orientia tsutsugamushi by flow cytometry reported in other studies were used as the control group in our study and, similar to the previously reported results, reduction of the growth of Orientia tsutsugamushi in response to interferon-γ administration was confirmed (Fig. 4).8

Bottom Line: Treatment of the cells with dexamethasone at concentrations of 10¹ and 10⁷ pg/mL showed no influence on the growth of Orientia tsutsugamushi.Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro.Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Yonsei University College of Medicine, 250 Seongsan-ro, Seodaemun-gu, Seoul 120-752, Korea.

ABSTRACT

Purpose: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease.

Materials and methods: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 10¹ and 10⁷ pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone.

Results: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fluorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 10¹ and 10⁷ pg/mL showed no influence on the growth of Orientia tsutsugamushi.

Conclusion: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

Show MeSH
Related in: MedlinePlus