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Dynamic variations in the peripheral blood lymphocyte subgroups of patients with 2009 pandemic H1N1 swine-origin influenza A virus infection.

Guo X, Chen Y, Li X, Kong H, Yang S, Ye B, Cui D, Wu W, Li L - Virol. J. (2011)

Bottom Line: The frequency of NK cells were decreased in all cases and CD19(+) B cells were increased in severe cases than those of the controls.Serum cytokines, specifically IL-2, IL-4, IL-6, IL-10, and IFN-γ exhibited no significant change both in the moderate and the severe cases during the whole monitoring process.In the early stage of the disease, serum CRP levels in the severe and moderate groups were significantly higher than that in the control group.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, PR China.

ABSTRACT

Background: Novel Influenza A (H1N1) is an acute respiratory infectious disease. Animal experiments indicated that when H1N1 virus infected early hosts, it showed strong CD4(+), CD8(+), and CD4(+)CD25(+) T cell reactions. The aim of this study was to investigate the dynamic fluctuations of the peripheral blood lymphocyte subgroups in patients infected with H1N1 swine-origin influenza A virus (S-OIV).

Methods: The frequency of T cells, B cells, natural killer (NK) cells, and regulatory T cells (Treg) in 36 severe H1N1 and 40 moderate H1N1 patients were detected at different periods by flow cytometry. In parallel, serum cytokines were detected by enzyme-linked immunosorbent assay and C-reactive protein (CRP) was analyzed through an image-type automatic biochemical analyzer. In addition, 20 healthy volunteers, who were not infected with 2009 H1N1 virus, were selected as controls.

Results: The frequency of NK cells were decreased in all cases and CD19(+) B cells were increased in severe cases than those of the controls. At 1-2d from onset, the frequency of CD4(+) and CD4(+)CD25(+) T cells in moderate cases was higher than in the severe cases. Serum cytokines, specifically IL-2, IL-4, IL-6, IL-10, and IFN-γ exhibited no significant change both in the moderate and the severe cases during the whole monitoring process. In the early stage of the disease, serum CRP levels in the severe and moderate groups were significantly higher than that in the control group.

Conclusions: Patients showed different lymphocyte subgroup distributions between mild and severe cases, which might affect the incidence and development of 2009 H1N1.

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Related in: MedlinePlus

Analysis of the dynamic changes in the lymphocyte subgroup and C-reactive protein (CRP) in the peripheral blood of patients with Influenza A (H1N1) 2009. CD4+ T, CD8+ T, CD4+CD25+ Treg, NK, and CD19+ B cells are presented as a percentage of total peripheral blood leukocytes (PBLs) in severe H1N1 (n = 36) patients, moderate H1N1 (n = 40) patients, and healthy volunteers (n = 20). †: denotes statistically significant differences compared with severe H1N1; *: denotes statistically significant differences compared with the healthy volunteers; **: denotes statistically significant differences compared with the moderate H1N1 patients and with the healthy volunteers.
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Figure 1: Analysis of the dynamic changes in the lymphocyte subgroup and C-reactive protein (CRP) in the peripheral blood of patients with Influenza A (H1N1) 2009. CD4+ T, CD8+ T, CD4+CD25+ Treg, NK, and CD19+ B cells are presented as a percentage of total peripheral blood leukocytes (PBLs) in severe H1N1 (n = 36) patients, moderate H1N1 (n = 40) patients, and healthy volunteers (n = 20). †: denotes statistically significant differences compared with severe H1N1; *: denotes statistically significant differences compared with the healthy volunteers; **: denotes statistically significant differences compared with the moderate H1N1 patients and with the healthy volunteers.

Mentions: The frequency of the different lymphocyte subsets at different periods were statistically compared and the results show that the frequency of peripheral CD4+ and CD4+CD25+ T cells in the moderate H1N1 patients were higher than that of the severe H1N1 patients within the first 1-2 d (Figures 1a and 1c; P < 0.05). The CD19+ B cell counts of the severe H1N1 patients were significantly higher than those of the moderate H1N1 patients and the control group for the same period (Figure 1e), whereas the NK cell counts were significantly less than that of the control group (Figure 1d; P < 0.05). The frequency of CD8+ T cells had no significant difference (P > 0.05; Figure 1b) among the three groups.


Dynamic variations in the peripheral blood lymphocyte subgroups of patients with 2009 pandemic H1N1 swine-origin influenza A virus infection.

Guo X, Chen Y, Li X, Kong H, Yang S, Ye B, Cui D, Wu W, Li L - Virol. J. (2011)

Analysis of the dynamic changes in the lymphocyte subgroup and C-reactive protein (CRP) in the peripheral blood of patients with Influenza A (H1N1) 2009. CD4+ T, CD8+ T, CD4+CD25+ Treg, NK, and CD19+ B cells are presented as a percentage of total peripheral blood leukocytes (PBLs) in severe H1N1 (n = 36) patients, moderate H1N1 (n = 40) patients, and healthy volunteers (n = 20). †: denotes statistically significant differences compared with severe H1N1; *: denotes statistically significant differences compared with the healthy volunteers; **: denotes statistically significant differences compared with the moderate H1N1 patients and with the healthy volunteers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104371&req=5

Figure 1: Analysis of the dynamic changes in the lymphocyte subgroup and C-reactive protein (CRP) in the peripheral blood of patients with Influenza A (H1N1) 2009. CD4+ T, CD8+ T, CD4+CD25+ Treg, NK, and CD19+ B cells are presented as a percentage of total peripheral blood leukocytes (PBLs) in severe H1N1 (n = 36) patients, moderate H1N1 (n = 40) patients, and healthy volunteers (n = 20). †: denotes statistically significant differences compared with severe H1N1; *: denotes statistically significant differences compared with the healthy volunteers; **: denotes statistically significant differences compared with the moderate H1N1 patients and with the healthy volunteers.
Mentions: The frequency of the different lymphocyte subsets at different periods were statistically compared and the results show that the frequency of peripheral CD4+ and CD4+CD25+ T cells in the moderate H1N1 patients were higher than that of the severe H1N1 patients within the first 1-2 d (Figures 1a and 1c; P < 0.05). The CD19+ B cell counts of the severe H1N1 patients were significantly higher than those of the moderate H1N1 patients and the control group for the same period (Figure 1e), whereas the NK cell counts were significantly less than that of the control group (Figure 1d; P < 0.05). The frequency of CD8+ T cells had no significant difference (P > 0.05; Figure 1b) among the three groups.

Bottom Line: The frequency of NK cells were decreased in all cases and CD19(+) B cells were increased in severe cases than those of the controls.Serum cytokines, specifically IL-2, IL-4, IL-6, IL-10, and IFN-γ exhibited no significant change both in the moderate and the severe cases during the whole monitoring process.In the early stage of the disease, serum CRP levels in the severe and moderate groups were significantly higher than that in the control group.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, PR China.

ABSTRACT

Background: Novel Influenza A (H1N1) is an acute respiratory infectious disease. Animal experiments indicated that when H1N1 virus infected early hosts, it showed strong CD4(+), CD8(+), and CD4(+)CD25(+) T cell reactions. The aim of this study was to investigate the dynamic fluctuations of the peripheral blood lymphocyte subgroups in patients infected with H1N1 swine-origin influenza A virus (S-OIV).

Methods: The frequency of T cells, B cells, natural killer (NK) cells, and regulatory T cells (Treg) in 36 severe H1N1 and 40 moderate H1N1 patients were detected at different periods by flow cytometry. In parallel, serum cytokines were detected by enzyme-linked immunosorbent assay and C-reactive protein (CRP) was analyzed through an image-type automatic biochemical analyzer. In addition, 20 healthy volunteers, who were not infected with 2009 H1N1 virus, were selected as controls.

Results: The frequency of NK cells were decreased in all cases and CD19(+) B cells were increased in severe cases than those of the controls. At 1-2d from onset, the frequency of CD4(+) and CD4(+)CD25(+) T cells in moderate cases was higher than in the severe cases. Serum cytokines, specifically IL-2, IL-4, IL-6, IL-10, and IFN-γ exhibited no significant change both in the moderate and the severe cases during the whole monitoring process. In the early stage of the disease, serum CRP levels in the severe and moderate groups were significantly higher than that in the control group.

Conclusions: Patients showed different lymphocyte subgroup distributions between mild and severe cases, which might affect the incidence and development of 2009 H1N1.

Show MeSH
Related in: MedlinePlus