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The protective effect of Malva sylvestris on rat kidney damaged by vanadium.

Marouane W, Soussi A, Murat JC, Bezzine S, El Feki A - Lipids Health Dis (2011)

Bottom Line: These properties were confirmed in Malva sylvestris by two separate methods; namely, the Diphenyl-2-picrylhydrazyl assay and the Nitroblue Tetrazolium reduction assay.A significant increase in the formation of free radicals and antioxidant enzyme activities was noticed.Malva sylvestris is proved to have a high antioxidative potential thanks to its richness in phenolic compounds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratoire d’Ecophysiologie Animale, Faculté des Sciences, Route de Soukra 3038 Sfax-University of Sfax-Tunisia.

ABSTRACT

Background: The protective effect of the common mallow (Malva sylvestris) decoction on renal damages in rats induced by ammonium metavanadate poisoning was evaluated. On the one hand, vanadium toxicity is associated to the production of reactive oxygen species, causing a lipid peroxidation and an alteration in the enzymatic antioxidant defence. On the other hand, many medicinal plants are known to possess antioxidant and radical scavenging properties, thanks to the presence of flavonoids. These properties were confirmed in Malva sylvestris by two separate methods; namely, the Diphenyl-2-picrylhydrazyl assay and the Nitroblue Tetrazolium reduction assay.

Results: In 80 rats exposed to ammonium metavanadate (0.24 mmol/kg body weight in drinking water) for 90 days, lipid peroxidation levels and superoxide dismutase, catalase and glutathione peroxidase activities were measured in kidney. A significant increase in the formation of free radicals and antioxidant enzyme activities was noticed. In addition, a histological examination of kidney revealed a structural deterioration of the renal cortical capsules and a shrinking of the Bowman space. In animals intoxicated by metavanadate but also given a Malva sylvestris decoction (0.2 g dry mallow/kg body weight), no such pathologic features were observed: lipid peroxidation levels, antioxidant enzyme activities and histological features appeared normal as compared to control rats.

Conclusion: Malva sylvestris is proved to have a high antioxidative potential thanks to its richness in phenolic compounds.

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Related in: MedlinePlus

Activities of some enzymes in controls and treated rats after 15, 30, 60 and 90 days. (A) Superoxide dismutase (SOD), (B) catalase (CAT) and (C) Glutathione peroxidase (GPX). Values are the mean ± SD, normalized to 100 for controls (n = 5). Student's test was used for comparison: * (p < 0.05)
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Figure 4: Activities of some enzymes in controls and treated rats after 15, 30, 60 and 90 days. (A) Superoxide dismutase (SOD), (B) catalase (CAT) and (C) Glutathione peroxidase (GPX). Values are the mean ± SD, normalized to 100 for controls (n = 5). Student's test was used for comparison: * (p < 0.05)

Mentions: Activities of some major enzymes involved in the defence against oxidative stress, SOD, CAT and GPX, were measured in control and treated rats using the methods of Asada, Aebi and Paglia respectively [20-22]. As shown in Figure 4, vanadium poisoning resulted in an increased activity of kidney SOD, CAT and GPX activities at day 30 of treatment with 74.5%, 31.5% and 82% respectively.


The protective effect of Malva sylvestris on rat kidney damaged by vanadium.

Marouane W, Soussi A, Murat JC, Bezzine S, El Feki A - Lipids Health Dis (2011)

Activities of some enzymes in controls and treated rats after 15, 30, 60 and 90 days. (A) Superoxide dismutase (SOD), (B) catalase (CAT) and (C) Glutathione peroxidase (GPX). Values are the mean ± SD, normalized to 100 for controls (n = 5). Student's test was used for comparison: * (p < 0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3104358&req=5

Figure 4: Activities of some enzymes in controls and treated rats after 15, 30, 60 and 90 days. (A) Superoxide dismutase (SOD), (B) catalase (CAT) and (C) Glutathione peroxidase (GPX). Values are the mean ± SD, normalized to 100 for controls (n = 5). Student's test was used for comparison: * (p < 0.05)
Mentions: Activities of some major enzymes involved in the defence against oxidative stress, SOD, CAT and GPX, were measured in control and treated rats using the methods of Asada, Aebi and Paglia respectively [20-22]. As shown in Figure 4, vanadium poisoning resulted in an increased activity of kidney SOD, CAT and GPX activities at day 30 of treatment with 74.5%, 31.5% and 82% respectively.

Bottom Line: These properties were confirmed in Malva sylvestris by two separate methods; namely, the Diphenyl-2-picrylhydrazyl assay and the Nitroblue Tetrazolium reduction assay.A significant increase in the formation of free radicals and antioxidant enzyme activities was noticed.Malva sylvestris is proved to have a high antioxidative potential thanks to its richness in phenolic compounds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratoire d’Ecophysiologie Animale, Faculté des Sciences, Route de Soukra 3038 Sfax-University of Sfax-Tunisia.

ABSTRACT

Background: The protective effect of the common mallow (Malva sylvestris) decoction on renal damages in rats induced by ammonium metavanadate poisoning was evaluated. On the one hand, vanadium toxicity is associated to the production of reactive oxygen species, causing a lipid peroxidation and an alteration in the enzymatic antioxidant defence. On the other hand, many medicinal plants are known to possess antioxidant and radical scavenging properties, thanks to the presence of flavonoids. These properties were confirmed in Malva sylvestris by two separate methods; namely, the Diphenyl-2-picrylhydrazyl assay and the Nitroblue Tetrazolium reduction assay.

Results: In 80 rats exposed to ammonium metavanadate (0.24 mmol/kg body weight in drinking water) for 90 days, lipid peroxidation levels and superoxide dismutase, catalase and glutathione peroxidase activities were measured in kidney. A significant increase in the formation of free radicals and antioxidant enzyme activities was noticed. In addition, a histological examination of kidney revealed a structural deterioration of the renal cortical capsules and a shrinking of the Bowman space. In animals intoxicated by metavanadate but also given a Malva sylvestris decoction (0.2 g dry mallow/kg body weight), no such pathologic features were observed: lipid peroxidation levels, antioxidant enzyme activities and histological features appeared normal as compared to control rats.

Conclusion: Malva sylvestris is proved to have a high antioxidative potential thanks to its richness in phenolic compounds.

Show MeSH
Related in: MedlinePlus