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Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

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Related in: MedlinePlus

β-glucan increases the allo-proliferative capability of DCs. DC cultures were established and treated as described in the legend for Fig. 1. For the allo-proliferation assay, DCs treated with β-glucan (1 × 104 cells/well) were co-cultured for 5 days with allogeneic splenocytes (2 × 105 cells/well) stained with 5 µM carboxyfluorescein succinimidyl ester. The number indicates the percentage of proliferating cells with a low FL1 value in the co-culture.
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Figure 5: β-glucan increases the allo-proliferative capability of DCs. DC cultures were established and treated as described in the legend for Fig. 1. For the allo-proliferation assay, DCs treated with β-glucan (1 × 104 cells/well) were co-cultured for 5 days with allogeneic splenocytes (2 × 105 cells/well) stained with 5 µM carboxyfluorescein succinimidyl ester. The number indicates the percentage of proliferating cells with a low FL1 value in the co-culture.

Mentions: To confirm the antigen-presenting capability of DCs treated with β-glucan, we prepared allogeneic splenocytes from BALB/c mice [7]. The cells were then stained with carboxyfluorescein succinimidyl ester (CFSE) and co-cultured with immature or β-glucan-treated DCs. Highly proliferative cells had a relatively low CFSE intensity detected as FL1 by flow cytometric analysis because of their high cell division rates. Splenocytes cocultured with DCs treated with β-glucan (100 µg/mL) showed the lowest CFSE intensity, indicating that these cells had the highest proliferative capacity (Fig. 5). These observations showed that β-glucan enhances the antigen-presenting capability of DCs. Taken together, our results demonstrated that β-glucan from P. polymyxa JB115 induces the maturation of DCs, the most potent antigen-presenting cells in host immunity. This study may provide researchers with valuable information to increase the use of β-glucan.


Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

β-glucan increases the allo-proliferative capability of DCs. DC cultures were established and treated as described in the legend for Fig. 1. For the allo-proliferation assay, DCs treated with β-glucan (1 × 104 cells/well) were co-cultured for 5 days with allogeneic splenocytes (2 × 105 cells/well) stained with 5 µM carboxyfluorescein succinimidyl ester. The number indicates the percentage of proliferating cells with a low FL1 value in the co-culture.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104174&req=5

Figure 5: β-glucan increases the allo-proliferative capability of DCs. DC cultures were established and treated as described in the legend for Fig. 1. For the allo-proliferation assay, DCs treated with β-glucan (1 × 104 cells/well) were co-cultured for 5 days with allogeneic splenocytes (2 × 105 cells/well) stained with 5 µM carboxyfluorescein succinimidyl ester. The number indicates the percentage of proliferating cells with a low FL1 value in the co-culture.
Mentions: To confirm the antigen-presenting capability of DCs treated with β-glucan, we prepared allogeneic splenocytes from BALB/c mice [7]. The cells were then stained with carboxyfluorescein succinimidyl ester (CFSE) and co-cultured with immature or β-glucan-treated DCs. Highly proliferative cells had a relatively low CFSE intensity detected as FL1 by flow cytometric analysis because of their high cell division rates. Splenocytes cocultured with DCs treated with β-glucan (100 µg/mL) showed the lowest CFSE intensity, indicating that these cells had the highest proliferative capacity (Fig. 5). These observations showed that β-glucan enhances the antigen-presenting capability of DCs. Taken together, our results demonstrated that β-glucan from P. polymyxa JB115 induces the maturation of DCs, the most potent antigen-presenting cells in host immunity. This study may provide researchers with valuable information to increase the use of β-glucan.

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

Show MeSH
Related in: MedlinePlus