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Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

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β-glucan enhances the expression of immune-related DC surface markers but decreases DC antigen uptake. DCs were prepared and treated as described in the legend for Fig. 1. For surface marker analysis (A), the number in the histogram indicates the mean fluorescence intensity (MFI) of the main DC population. For antigen uptake analysis (B), DCs were incubated with 250 µg/mL dextran-fluorescein isothiocyanate at 4℃ or 37℃ for 45 min. The number in the histogram indicates the MFI of viable DCs.
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Figure 4: β-glucan enhances the expression of immune-related DC surface markers but decreases DC antigen uptake. DCs were prepared and treated as described in the legend for Fig. 1. For surface marker analysis (A), the number in the histogram indicates the mean fluorescence intensity (MFI) of the main DC population. For antigen uptake analysis (B), DCs were incubated with 250 µg/mL dextran-fluorescein isothiocyanate at 4℃ or 37℃ for 45 min. The number in the histogram indicates the MFI of viable DCs.

Mentions: To determine whether β-glucan induces the maturation of DCs, expression levels of immune-related DC surface markers were measured by flow cytometric analysis [9]. Major histocompatibility complex (MHC) class I and II are major antigen-presenting molecules on DCs while CD54 and CD86 are adhesion and co-stimulatory molecules, respectively [11]. β-glucan consistently enhanced the expression all of the surface markers investigated in the present study (Fig. 4A). Because MHC class II and CD86 molecules have been identified as maturation markers of bone marrow-derived DCs [6,12], β-glucan may induce the maturation of DCs. Decreased antigen uptake is a major characteristic of mature DCs. Antigen uptake analysis using dextran-fluorescein isothiocyanate revealed that β-glucan profoundly decreased the antigen uptake capability of DCs compared to immature DCs (Fig. 4B). Zymosan was used as a positive control in this assay.


Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

β-glucan enhances the expression of immune-related DC surface markers but decreases DC antigen uptake. DCs were prepared and treated as described in the legend for Fig. 1. For surface marker analysis (A), the number in the histogram indicates the mean fluorescence intensity (MFI) of the main DC population. For antigen uptake analysis (B), DCs were incubated with 250 µg/mL dextran-fluorescein isothiocyanate at 4℃ or 37℃ for 45 min. The number in the histogram indicates the MFI of viable DCs.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104174&req=5

Figure 4: β-glucan enhances the expression of immune-related DC surface markers but decreases DC antigen uptake. DCs were prepared and treated as described in the legend for Fig. 1. For surface marker analysis (A), the number in the histogram indicates the mean fluorescence intensity (MFI) of the main DC population. For antigen uptake analysis (B), DCs were incubated with 250 µg/mL dextran-fluorescein isothiocyanate at 4℃ or 37℃ for 45 min. The number in the histogram indicates the MFI of viable DCs.
Mentions: To determine whether β-glucan induces the maturation of DCs, expression levels of immune-related DC surface markers were measured by flow cytometric analysis [9]. Major histocompatibility complex (MHC) class I and II are major antigen-presenting molecules on DCs while CD54 and CD86 are adhesion and co-stimulatory molecules, respectively [11]. β-glucan consistently enhanced the expression all of the surface markers investigated in the present study (Fig. 4A). Because MHC class II and CD86 molecules have been identified as maturation markers of bone marrow-derived DCs [6,12], β-glucan may induce the maturation of DCs. Decreased antigen uptake is a major characteristic of mature DCs. Antigen uptake analysis using dextran-fluorescein isothiocyanate revealed that β-glucan profoundly decreased the antigen uptake capability of DCs compared to immature DCs (Fig. 4B). Zymosan was used as a positive control in this assay.

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

Show MeSH
Related in: MedlinePlus