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Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

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Related in: MedlinePlus

β-glucan increases cytokine and nitric oxide production of DCs. DCs were seeded and treated as described in Fig. 2. The amounts of interleukin (IL)-12 (A), tumor necrosis factor (TNF)-α (B), and nitric oxide (C) produced by DCs were measured. Data are presented as the mean ± SD from four individual wells. **p < 0.01, ***p < 0.001.
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Figure 3: β-glucan increases cytokine and nitric oxide production of DCs. DCs were seeded and treated as described in Fig. 2. The amounts of interleukin (IL)-12 (A), tumor necrosis factor (TNF)-α (B), and nitric oxide (C) produced by DCs were measured. Data are presented as the mean ± SD from four individual wells. **p < 0.01, ***p < 0.001.

Mentions: The production of tumor necrosis factor (TNF)-α and interleukin (IL)-12 by DCs treated with β-glucan was determined by an ELISA. IL-12 and TNF-α are representative cytokines involved in cell-mediated and innate immunity, respectively, along with DC survival [3,5]. β-glucan (100 µg/mL) significantly increased the production of both cytokines (Figs. 3A and B). In addition, β-glucan enhanced the release of nitric oxide from DCs (Fig. 3C). Nitric oxide plays a critical role in eliminating intracellular pathogens in macrophages.


Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

β-glucan increases cytokine and nitric oxide production of DCs. DCs were seeded and treated as described in Fig. 2. The amounts of interleukin (IL)-12 (A), tumor necrosis factor (TNF)-α (B), and nitric oxide (C) produced by DCs were measured. Data are presented as the mean ± SD from four individual wells. **p < 0.01, ***p < 0.001.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104174&req=5

Figure 3: β-glucan increases cytokine and nitric oxide production of DCs. DCs were seeded and treated as described in Fig. 2. The amounts of interleukin (IL)-12 (A), tumor necrosis factor (TNF)-α (B), and nitric oxide (C) produced by DCs were measured. Data are presented as the mean ± SD from four individual wells. **p < 0.01, ***p < 0.001.
Mentions: The production of tumor necrosis factor (TNF)-α and interleukin (IL)-12 by DCs treated with β-glucan was determined by an ELISA. IL-12 and TNF-α are representative cytokines involved in cell-mediated and innate immunity, respectively, along with DC survival [3,5]. β-glucan (100 µg/mL) significantly increased the production of both cytokines (Figs. 3A and B). In addition, β-glucan enhanced the release of nitric oxide from DCs (Fig. 3C). Nitric oxide plays a critical role in eliminating intracellular pathogens in macrophages.

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

Show MeSH
Related in: MedlinePlus