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Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

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Clusters of dendritic cells (DCs) treated with β-glucan. DCs were seeded at a density of 5 × 105 cells/mL in 6-well culture plates, and then treated with β-glucan for 2 days. (A) DC morphology. ×200. (B) The number and size of DCs was measured by flow cytometric analysis.
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Figure 1: Clusters of dendritic cells (DCs) treated with β-glucan. DCs were seeded at a density of 5 × 105 cells/mL in 6-well culture plates, and then treated with β-glucan for 2 days. (A) DC morphology. ×200. (B) The number and size of DCs was measured by flow cytometric analysis.

Mentions: To investigate the effects of β-glucan purified from P. polymyxa JB115 on DCs, we prepared immature DCs from bone marrow as previously described [10], and treated these cells with 1~100 µg/mL β-glucan. Two days after β-glucan treatment, clusters of DCs were observed using an inverted microscope connected to a digital camera (Fig. 1A). In addition, cell size was analyzed using flow cytometry. It was found that β-glucan enhanced the percentage of normal-sized DCs in the forward scatter/side scatter dot plot (Fig. 1B). A cell viability assay using a trypan blue solution demonstrated that 100 µg/mL β-glucan significantly increased the viability of DCs (Fig. 2). Thus, β-glucan may enhance the survival of DCs and also helps maintain normal cell size.


Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.

Ko EJ, Byon YY, Jee Y, Shin T, Park SC, Hahn TW, Joo HG - J. Vet. Sci. (2011)

Clusters of dendritic cells (DCs) treated with β-glucan. DCs were seeded at a density of 5 × 105 cells/mL in 6-well culture plates, and then treated with β-glucan for 2 days. (A) DC morphology. ×200. (B) The number and size of DCs was measured by flow cytometric analysis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104174&req=5

Figure 1: Clusters of dendritic cells (DCs) treated with β-glucan. DCs were seeded at a density of 5 × 105 cells/mL in 6-well culture plates, and then treated with β-glucan for 2 days. (A) DC morphology. ×200. (B) The number and size of DCs was measured by flow cytometric analysis.
Mentions: To investigate the effects of β-glucan purified from P. polymyxa JB115 on DCs, we prepared immature DCs from bone marrow as previously described [10], and treated these cells with 1~100 µg/mL β-glucan. Two days after β-glucan treatment, clusters of DCs were observed using an inverted microscope connected to a digital camera (Fig. 1A). In addition, cell size was analyzed using flow cytometry. It was found that β-glucan enhanced the percentage of normal-sized DCs in the forward scatter/side scatter dot plot (Fig. 1B). A cell viability assay using a trypan blue solution demonstrated that 100 µg/mL β-glucan significantly increased the viability of DCs (Fig. 2). Thus, β-glucan may enhance the survival of DCs and also helps maintain normal cell size.

Bottom Line: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers.In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone.These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.

ABSTRACT
We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.

Show MeSH
Related in: MedlinePlus