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Establishment of a standard operating procedure for predicting the time of calving in cattle.

Streyl D, Sauter-Louis C, Braunert A, Lange D, Weber F, Zerbe H - J. Vet. Sci. (2011)

Bottom Line: The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either calving or no calving within 12 h.By combining the PS and PRBT (if PS ≥ 4), the prediction of calving within the next 12 h improved from 14.9% to 53.1%, and the probability of ruling out calving was 96.8%.The PRBT was compared to the results of an enzyme immunoassay (sensitivity, 90.2%; specificity, 74.9%).

View Article: PubMed Central - PubMed

Affiliation: Clinic for Ruminants with Ambulatory and Herd Health Services, Faculty of Veterinary Medicine, Ludwig-Maximilian University of Munich, Sonnenstr. 16, 85764 Oberschleissheim, Germany. d.streyl@lmu.de

ABSTRACT
Precise calving monitoring is essential for minimizing the effects of dystocia in cows and calves. We conducted two studies in healthy cows that compared seven clinical signs (broad pelvic ligaments relaxation, vaginal secretion, udder hyperplasia, udder edema, teat filling, tail relaxation, and vulva edema) alone and in combination in order to predict the time of parturition. The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either calving or no calving within 12 h. For the proposed parturition score (PS), a threshold of 4 PS points was identified below which calving within the next 12 h could be ruled out with a probability of 99.3% in cows (95.5% in heifers). Above this threshold, intermitted calving monitoring every 3 h and a progesterone rapid blood test (PRBT) would be recommended. By combining the PS and PRBT (if PS ≥ 4), the prediction of calving within the next 12 h improved from 14.9% to 53.1%, and the probability of ruling out calving was 96.8%. The PRBT was compared to the results of an enzyme immunoassay (sensitivity, 90.2%; specificity, 74.9%). The standard operating procedure developed in this study that combines the PS and PRBT will enable veterinarians to rule out or predict calving within a 12 h period in cows with high accuracy under field conditions.

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Related in: MedlinePlus

Evaluation of a commercially available progesterone rapid blood test (PRBT) during the prepartum period. Progesterone was measured by an enzyme immunoassay as the gold standard and compared to semi-quantitative PRBT. The threshold progesterone level for an active corpus luteum (CL) is reported in the literature to be 1.2 ng/mL. The PRBT could differentiate between low (progesterone below 1 ng/mL) and high (progesterone above 1.2 ng/mL) CL activity. The sensitivity of the PRBT was 90.2% and the specificity 74.9%. Each diamond corresponds to a single blood sample.
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Figure 3: Evaluation of a commercially available progesterone rapid blood test (PRBT) during the prepartum period. Progesterone was measured by an enzyme immunoassay as the gold standard and compared to semi-quantitative PRBT. The threshold progesterone level for an active corpus luteum (CL) is reported in the literature to be 1.2 ng/mL. The PRBT could differentiate between low (progesterone below 1 ng/mL) and high (progesterone above 1.2 ng/mL) CL activity. The sensitivity of the PRBT was 90.2% and the specificity 74.9%. Each diamond corresponds to a single blood sample.

Mentions: As shown above, the reduction in progesterone levels was the most precise and objective indication of calving. An on-farm PRBT for cycling cows was tested to see if it could be used as a predictive tool during the prepartal period. We compared this semi-quantitative PRBT with the standard quantitative progesterone EIA analysis. The PRBT had a sensitivity of 90.2% and a specificity of 74.9% for detecting progesterone levels higher or lower than 1.2 ng/mL (Fig. 3).


Establishment of a standard operating procedure for predicting the time of calving in cattle.

Streyl D, Sauter-Louis C, Braunert A, Lange D, Weber F, Zerbe H - J. Vet. Sci. (2011)

Evaluation of a commercially available progesterone rapid blood test (PRBT) during the prepartum period. Progesterone was measured by an enzyme immunoassay as the gold standard and compared to semi-quantitative PRBT. The threshold progesterone level for an active corpus luteum (CL) is reported in the literature to be 1.2 ng/mL. The PRBT could differentiate between low (progesterone below 1 ng/mL) and high (progesterone above 1.2 ng/mL) CL activity. The sensitivity of the PRBT was 90.2% and the specificity 74.9%. Each diamond corresponds to a single blood sample.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104173&req=5

Figure 3: Evaluation of a commercially available progesterone rapid blood test (PRBT) during the prepartum period. Progesterone was measured by an enzyme immunoassay as the gold standard and compared to semi-quantitative PRBT. The threshold progesterone level for an active corpus luteum (CL) is reported in the literature to be 1.2 ng/mL. The PRBT could differentiate between low (progesterone below 1 ng/mL) and high (progesterone above 1.2 ng/mL) CL activity. The sensitivity of the PRBT was 90.2% and the specificity 74.9%. Each diamond corresponds to a single blood sample.
Mentions: As shown above, the reduction in progesterone levels was the most precise and objective indication of calving. An on-farm PRBT for cycling cows was tested to see if it could be used as a predictive tool during the prepartal period. We compared this semi-quantitative PRBT with the standard quantitative progesterone EIA analysis. The PRBT had a sensitivity of 90.2% and a specificity of 74.9% for detecting progesterone levels higher or lower than 1.2 ng/mL (Fig. 3).

Bottom Line: The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either calving or no calving within 12 h.By combining the PS and PRBT (if PS ≥ 4), the prediction of calving within the next 12 h improved from 14.9% to 53.1%, and the probability of ruling out calving was 96.8%.The PRBT was compared to the results of an enzyme immunoassay (sensitivity, 90.2%; specificity, 74.9%).

View Article: PubMed Central - PubMed

Affiliation: Clinic for Ruminants with Ambulatory and Herd Health Services, Faculty of Veterinary Medicine, Ludwig-Maximilian University of Munich, Sonnenstr. 16, 85764 Oberschleissheim, Germany. d.streyl@lmu.de

ABSTRACT
Precise calving monitoring is essential for minimizing the effects of dystocia in cows and calves. We conducted two studies in healthy cows that compared seven clinical signs (broad pelvic ligaments relaxation, vaginal secretion, udder hyperplasia, udder edema, teat filling, tail relaxation, and vulva edema) alone and in combination in order to predict the time of parturition. The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either calving or no calving within 12 h. For the proposed parturition score (PS), a threshold of 4 PS points was identified below which calving within the next 12 h could be ruled out with a probability of 99.3% in cows (95.5% in heifers). Above this threshold, intermitted calving monitoring every 3 h and a progesterone rapid blood test (PRBT) would be recommended. By combining the PS and PRBT (if PS ≥ 4), the prediction of calving within the next 12 h improved from 14.9% to 53.1%, and the probability of ruling out calving was 96.8%. The PRBT was compared to the results of an enzyme immunoassay (sensitivity, 90.2%; specificity, 74.9%). The standard operating procedure developed in this study that combines the PS and PRBT will enable veterinarians to rule out or predict calving within a 12 h period in cows with high accuracy under field conditions.

Show MeSH
Related in: MedlinePlus