Limits...
Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.

Kwon JS, Park RK, Shim TJ, Jeong MH, Cho MC, Ahn Y, Kim DW - J. Vet. Sci. (2011)

Bottom Line: Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g).On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group.This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Chonnam National University Hospital, Gwanju 501-757, Korea.

ABSTRACT
The purpose of our study was to create a novel rat aorta stent implantation model. Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g). Two and four weeks after stent implantation, the aorta were collected, fixed with 2% glutaraldehyde, and cut into two segments. One segment was used for scanning electron microscopy analysis to evaluate re-endothelialization, and the other segment was used to calculate the neointimal area. At 2 weeks after stenting, the appearance of neointimal hyperplasia was less in the PES group than in the BMS group. At 4 weeks after stenting, no significant difference in neointimal hyperplasia was observed between two groups. On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group. This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

Show MeSH

Related in: MedlinePlus

Scanning electron microscopy photographs of BMS and PES in rat aorta. (A) Representation of strut area (blue) and uncovered area (red). (B) BMS and PES at 2 wk and (C) at 4 wk. (D) The covered stent ratio at 4 wk after stent implantation. (E) High magnification. Endothelial cells (green arrow), inflammatory cells (yellow arrow), and stent strut (red arrow). *p < 0.05. Ba and Ea: BMS 2 wk, Bb and Eb: PES 2 wk, Ca and Ec: BMS 4 wk, Cb and Ed: PES 4 wk. Scale bars = Aa: 500 µm, B and C: 1 mm, Ea and b: 100 µm, Ec and d: 50 µm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3104168&req=5

Figure 4: Scanning electron microscopy photographs of BMS and PES in rat aorta. (A) Representation of strut area (blue) and uncovered area (red). (B) BMS and PES at 2 wk and (C) at 4 wk. (D) The covered stent ratio at 4 wk after stent implantation. (E) High magnification. Endothelial cells (green arrow), inflammatory cells (yellow arrow), and stent strut (red arrow). *p < 0.05. Ba and Ea: BMS 2 wk, Bb and Eb: PES 2 wk, Ca and Ec: BMS 4 wk, Cb and Ed: PES 4 wk. Scale bars = Aa: 500 µm, B and C: 1 mm, Ea and b: 100 µm, Ec and d: 50 µm.

Mentions: The other segment of the stented aorta was placed in 10% formalin for an additional 24 h. Cross-sections were prepared from the segment by embedding the tissue in acrylic plastic and cutting sections 75 µm-thick. All sections were stained with hematoxylin and eosin. Histomorphometric analysis was carried out using Nis-Elements. The neointimal area was calculated as the media area minus the inner luminal area (neointimal area = media area - inner luminal area). These areas were determined in each section, averaged, and expressed as the absolute area in mm2. Additionally, the uncovered strut ratio was determined by microscopic observation and calculated as exposed strut number / total strut number. The focal thrombus ratio was calculated as thrombus-containing strut number / total strut number as found by microscopic observation. These were averaged over all stained sections. At SEM analysis, the re-endothelization percentage (%) was calculated as the strut area minus the uncovered area divided by the strut area (re-endothelization percentage (%) = strut area - uncovered area / strut area) (Fig. 4Ab).


Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.

Kwon JS, Park RK, Shim TJ, Jeong MH, Cho MC, Ahn Y, Kim DW - J. Vet. Sci. (2011)

Scanning electron microscopy photographs of BMS and PES in rat aorta. (A) Representation of strut area (blue) and uncovered area (red). (B) BMS and PES at 2 wk and (C) at 4 wk. (D) The covered stent ratio at 4 wk after stent implantation. (E) High magnification. Endothelial cells (green arrow), inflammatory cells (yellow arrow), and stent strut (red arrow). *p < 0.05. Ba and Ea: BMS 2 wk, Bb and Eb: PES 2 wk, Ca and Ec: BMS 4 wk, Cb and Ed: PES 4 wk. Scale bars = Aa: 500 µm, B and C: 1 mm, Ea and b: 100 µm, Ec and d: 50 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104168&req=5

Figure 4: Scanning electron microscopy photographs of BMS and PES in rat aorta. (A) Representation of strut area (blue) and uncovered area (red). (B) BMS and PES at 2 wk and (C) at 4 wk. (D) The covered stent ratio at 4 wk after stent implantation. (E) High magnification. Endothelial cells (green arrow), inflammatory cells (yellow arrow), and stent strut (red arrow). *p < 0.05. Ba and Ea: BMS 2 wk, Bb and Eb: PES 2 wk, Ca and Ec: BMS 4 wk, Cb and Ed: PES 4 wk. Scale bars = Aa: 500 µm, B and C: 1 mm, Ea and b: 100 µm, Ec and d: 50 µm.
Mentions: The other segment of the stented aorta was placed in 10% formalin for an additional 24 h. Cross-sections were prepared from the segment by embedding the tissue in acrylic plastic and cutting sections 75 µm-thick. All sections were stained with hematoxylin and eosin. Histomorphometric analysis was carried out using Nis-Elements. The neointimal area was calculated as the media area minus the inner luminal area (neointimal area = media area - inner luminal area). These areas were determined in each section, averaged, and expressed as the absolute area in mm2. Additionally, the uncovered strut ratio was determined by microscopic observation and calculated as exposed strut number / total strut number. The focal thrombus ratio was calculated as thrombus-containing strut number / total strut number as found by microscopic observation. These were averaged over all stained sections. At SEM analysis, the re-endothelization percentage (%) was calculated as the strut area minus the uncovered area divided by the strut area (re-endothelization percentage (%) = strut area - uncovered area / strut area) (Fig. 4Ab).

Bottom Line: Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g).On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group.This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Chonnam National University Hospital, Gwanju 501-757, Korea.

ABSTRACT
The purpose of our study was to create a novel rat aorta stent implantation model. Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g). Two and four weeks after stent implantation, the aorta were collected, fixed with 2% glutaraldehyde, and cut into two segments. One segment was used for scanning electron microscopy analysis to evaluate re-endothelialization, and the other segment was used to calculate the neointimal area. At 2 weeks after stenting, the appearance of neointimal hyperplasia was less in the PES group than in the BMS group. At 4 weeks after stenting, no significant difference in neointimal hyperplasia was observed between two groups. On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group. This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

Show MeSH
Related in: MedlinePlus