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Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.

Kwon JS, Park RK, Shim TJ, Jeong MH, Cho MC, Ahn Y, Kim DW - J. Vet. Sci. (2011)

Bottom Line: Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g).On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group.This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Chonnam National University Hospital, Gwanju 501-757, Korea.

ABSTRACT
The purpose of our study was to create a novel rat aorta stent implantation model. Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g). Two and four weeks after stent implantation, the aorta were collected, fixed with 2% glutaraldehyde, and cut into two segments. One segment was used for scanning electron microscopy analysis to evaluate re-endothelialization, and the other segment was used to calculate the neointimal area. At 2 weeks after stenting, the appearance of neointimal hyperplasia was less in the PES group than in the BMS group. At 4 weeks after stenting, no significant difference in neointimal hyperplasia was observed between two groups. On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group. This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

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Histomorphometric results of BMS or PES implantation. (A) Neointimal area. (B) Representation of neointimal area, media area (green line) and inner luminal area (square line). (C) Uncovered strut ratio. (D) Focal thrombus ratio. BMS 2 wk vs. PES 2 wk (*p < 0.05). BMS 4 wk vs. PES 4 wk (**p < 0.05), BMS 2 wk vs. BMS 4 wk (#p < 0.05), PES 2 wk vs. PES 4 wk (##p < 0.05).
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Figure 3: Histomorphometric results of BMS or PES implantation. (A) Neointimal area. (B) Representation of neointimal area, media area (green line) and inner luminal area (square line). (C) Uncovered strut ratio. (D) Focal thrombus ratio. BMS 2 wk vs. PES 2 wk (*p < 0.05). BMS 4 wk vs. PES 4 wk (**p < 0.05), BMS 2 wk vs. BMS 4 wk (#p < 0.05), PES 2 wk vs. PES 4 wk (##p < 0.05).

Mentions: The neointimal area was larger in the BMS group (0.262 ± 0.010 mm2) than in the PES group (0.170 ± 0.014 mm2) at 2 weeks following stent placement (p < 0.05) (Figs. 2A and 3A). However, no significant difference in the neointimal area was observed between the two groups [BMS group: 0.567 ± 0.055 mm2 vs. PES group: 0.618 ± 0.089 mm2 (p > 0.05)] at 4 weeks (Figs. 2A and 3C). The uncovered strut ratio was 38.3 ± 16.5% in the BMS group and 84.2 ± 1.1% in the PES group at 2 weeks, and 8.3 ± 4.0% in the BMS group and 45.0 ± 7.1% in the PES group at 4 weeks (Fig. 2B). The focal thrombus ratio was 0.0 ± 0.0% in the BMS group and 29.4 ± 8.9% in the PES group at 2 weeks and 5.0 ± 7.1% in the BMS group and 51.3 ± 8.3% in the PES group at 4 weeks. In addition, thrombi were detected only in the neointimal area (Figs. 2C and 3D).


Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.

Kwon JS, Park RK, Shim TJ, Jeong MH, Cho MC, Ahn Y, Kim DW - J. Vet. Sci. (2011)

Histomorphometric results of BMS or PES implantation. (A) Neointimal area. (B) Representation of neointimal area, media area (green line) and inner luminal area (square line). (C) Uncovered strut ratio. (D) Focal thrombus ratio. BMS 2 wk vs. PES 2 wk (*p < 0.05). BMS 4 wk vs. PES 4 wk (**p < 0.05), BMS 2 wk vs. BMS 4 wk (#p < 0.05), PES 2 wk vs. PES 4 wk (##p < 0.05).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104168&req=5

Figure 3: Histomorphometric results of BMS or PES implantation. (A) Neointimal area. (B) Representation of neointimal area, media area (green line) and inner luminal area (square line). (C) Uncovered strut ratio. (D) Focal thrombus ratio. BMS 2 wk vs. PES 2 wk (*p < 0.05). BMS 4 wk vs. PES 4 wk (**p < 0.05), BMS 2 wk vs. BMS 4 wk (#p < 0.05), PES 2 wk vs. PES 4 wk (##p < 0.05).
Mentions: The neointimal area was larger in the BMS group (0.262 ± 0.010 mm2) than in the PES group (0.170 ± 0.014 mm2) at 2 weeks following stent placement (p < 0.05) (Figs. 2A and 3A). However, no significant difference in the neointimal area was observed between the two groups [BMS group: 0.567 ± 0.055 mm2 vs. PES group: 0.618 ± 0.089 mm2 (p > 0.05)] at 4 weeks (Figs. 2A and 3C). The uncovered strut ratio was 38.3 ± 16.5% in the BMS group and 84.2 ± 1.1% in the PES group at 2 weeks, and 8.3 ± 4.0% in the BMS group and 45.0 ± 7.1% in the PES group at 4 weeks (Fig. 2B). The focal thrombus ratio was 0.0 ± 0.0% in the BMS group and 29.4 ± 8.9% in the PES group at 2 weeks and 5.0 ± 7.1% in the BMS group and 51.3 ± 8.3% in the PES group at 4 weeks. In addition, thrombi were detected only in the neointimal area (Figs. 2C and 3D).

Bottom Line: Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g).On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group.This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Chonnam National University Hospital, Gwanju 501-757, Korea.

ABSTRACT
The purpose of our study was to create a novel rat aorta stent implantation model. Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g). Two and four weeks after stent implantation, the aorta were collected, fixed with 2% glutaraldehyde, and cut into two segments. One segment was used for scanning electron microscopy analysis to evaluate re-endothelialization, and the other segment was used to calculate the neointimal area. At 2 weeks after stenting, the appearance of neointimal hyperplasia was less in the PES group than in the BMS group. At 4 weeks after stenting, no significant difference in neointimal hyperplasia was observed between two groups. On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group. This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

Show MeSH
Related in: MedlinePlus