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Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.

Kwon JS, Park RK, Shim TJ, Jeong MH, Cho MC, Ahn Y, Kim DW - J. Vet. Sci. (2011)

Bottom Line: Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g).On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group.This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Chonnam National University Hospital, Gwanju 501-757, Korea.

ABSTRACT
The purpose of our study was to create a novel rat aorta stent implantation model. Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g). Two and four weeks after stent implantation, the aorta were collected, fixed with 2% glutaraldehyde, and cut into two segments. One segment was used for scanning electron microscopy analysis to evaluate re-endothelialization, and the other segment was used to calculate the neointimal area. At 2 weeks after stenting, the appearance of neointimal hyperplasia was less in the PES group than in the BMS group. At 4 weeks after stenting, no significant difference in neointimal hyperplasia was observed between two groups. On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group. This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

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Related in: MedlinePlus

(A) 2 weeks (wk) and (B) 4 wk after implantation of the bare metal stent (BMS) or paclitaxel-eluting stent (PES). (C) Thrombus in the outer and inner area of the neointima at 4 wk. Scale bars = Aa and b, Ba and b: 1 mm; Ac and d, Bc and d, C: 50 µm. H&E stain.
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Figure 2: (A) 2 weeks (wk) and (B) 4 wk after implantation of the bare metal stent (BMS) or paclitaxel-eluting stent (PES). (C) Thrombus in the outer and inner area of the neointima at 4 wk. Scale bars = Aa and b, Ba and b: 1 mm; Ac and d, Bc and d, C: 50 µm. H&E stain.

Mentions: The neointimal area was larger in the BMS group (0.262 ± 0.010 mm2) than in the PES group (0.170 ± 0.014 mm2) at 2 weeks following stent placement (p < 0.05) (Figs. 2A and 3A). However, no significant difference in the neointimal area was observed between the two groups [BMS group: 0.567 ± 0.055 mm2 vs. PES group: 0.618 ± 0.089 mm2 (p > 0.05)] at 4 weeks (Figs. 2A and 3C). The uncovered strut ratio was 38.3 ± 16.5% in the BMS group and 84.2 ± 1.1% in the PES group at 2 weeks, and 8.3 ± 4.0% in the BMS group and 45.0 ± 7.1% in the PES group at 4 weeks (Fig. 2B). The focal thrombus ratio was 0.0 ± 0.0% in the BMS group and 29.4 ± 8.9% in the PES group at 2 weeks and 5.0 ± 7.1% in the BMS group and 51.3 ± 8.3% in the PES group at 4 weeks. In addition, thrombi were detected only in the neointimal area (Figs. 2C and 3D).


Comparison of bare metal stent and paclitaxel-eluting stent using a novel rat aorta stent model.

Kwon JS, Park RK, Shim TJ, Jeong MH, Cho MC, Ahn Y, Kim DW - J. Vet. Sci. (2011)

(A) 2 weeks (wk) and (B) 4 wk after implantation of the bare metal stent (BMS) or paclitaxel-eluting stent (PES). (C) Thrombus in the outer and inner area of the neointima at 4 wk. Scale bars = Aa and b, Ba and b: 1 mm; Ac and d, Bc and d, C: 50 µm. H&E stain.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104168&req=5

Figure 2: (A) 2 weeks (wk) and (B) 4 wk after implantation of the bare metal stent (BMS) or paclitaxel-eluting stent (PES). (C) Thrombus in the outer and inner area of the neointima at 4 wk. Scale bars = Aa and b, Ba and b: 1 mm; Ac and d, Bc and d, C: 50 µm. H&E stain.
Mentions: The neointimal area was larger in the BMS group (0.262 ± 0.010 mm2) than in the PES group (0.170 ± 0.014 mm2) at 2 weeks following stent placement (p < 0.05) (Figs. 2A and 3A). However, no significant difference in the neointimal area was observed between the two groups [BMS group: 0.567 ± 0.055 mm2 vs. PES group: 0.618 ± 0.089 mm2 (p > 0.05)] at 4 weeks (Figs. 2A and 3C). The uncovered strut ratio was 38.3 ± 16.5% in the BMS group and 84.2 ± 1.1% in the PES group at 2 weeks, and 8.3 ± 4.0% in the BMS group and 45.0 ± 7.1% in the PES group at 4 weeks (Fig. 2B). The focal thrombus ratio was 0.0 ± 0.0% in the BMS group and 29.4 ± 8.9% in the PES group at 2 weeks and 5.0 ± 7.1% in the BMS group and 51.3 ± 8.3% in the PES group at 4 weeks. In addition, thrombi were detected only in the neointimal area (Figs. 2C and 3D).

Bottom Line: Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g).On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group.This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Chonnam National University Hospital, Gwanju 501-757, Korea.

ABSTRACT
The purpose of our study was to create a novel rat aorta stent implantation model. Stainless steel bare metal stents (BMS) or paclitaxel-eluting stents (PES) were implanted in male Sprague-Dawley rats (BW 400 ± 20 g). Two and four weeks after stent implantation, the aorta were collected, fixed with 2% glutaraldehyde, and cut into two segments. One segment was used for scanning electron microscopy analysis to evaluate re-endothelialization, and the other segment was used to calculate the neointimal area. At 2 weeks after stenting, the appearance of neointimal hyperplasia was less in the PES group than in the BMS group. At 4 weeks after stenting, no significant difference in neointimal hyperplasia was observed between two groups. On the other hand, the PES group showed more thrombus formation and less re-endothelialization compared to the BMS group. This study demonstrated the ability of a novel rat model of aorta stenting via a common carotid artery to measure the efficacy and safety of commercially available drug-eluting stents.

Show MeSH
Related in: MedlinePlus