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Evaluation of the estrogenic effects of dietary perinatal Trifolium pratense.

Yatkin E, Daglioglu S - J. Vet. Sci. (2011)

Bottom Line: This study was designed to investigate the potential estrogenic effects of perinatal dietary phytoestrogens on the rat uterus.Our results revealed estrogen-like biological effects of perinatal T. pratense exposure.However, there were no statistically significant changes detected in the immunostaining intensity of ERα and PR between the groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey. emryat@gmail.com

ABSTRACT
This study was designed to investigate the potential estrogenic effects of perinatal dietary phytoestrogens on the rat uterus. Pregnant rats were divided to three groups provided the following diets: (1) rat chow, (2) rat chow with 7.5% Trifolium (T.) pratense, or (3) rat chow supplemented with 17β-estradiol (0.5 mg/kg). The dams in each group were kept on the same diet during pregnancy and lactation. Female offspring were euthanized on day 21 at which time body and organ weights were recorded and tissue samples were taken for histology. Immunohistochemistry was performed to detect estrogen receptor alpha (ERα) and progesterone receptor (PR) levels. Our results revealed estrogen-like biological effects of perinatal T. pratense exposure. Relative uterus and ovary weights in the experimental groups were increased compared to control. The number of uterine glands and luminal epithelium heights were also increased. However, there were no statistically significant changes detected in the immunostaining intensity of ERα and PR between the groups.

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ERα (A) and PR (B) immunostaining score in the uterus of 21-day-old rats. The proportion of positively stained cells was scored as follows: 0, none; 1, weak; 2, moderate; 3, strong; 4, intense staining.
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Figure 2: ERα (A) and PR (B) immunostaining score in the uterus of 21-day-old rats. The proportion of positively stained cells was scored as follows: 0, none; 1, weak; 2, moderate; 3, strong; 4, intense staining.

Mentions: ERα and PR positive nuclei were localized in the epithelial, stromal, and muscular cells of the uteri (Fig. 1). Uterine sections not receiving primary antibody showed no staining. Strong PR immunoactivity was observed in the luminal epithelium of uterine tubes but only faint staining was observed in T. pratense and estradiol treated groups (Fig. 1). The staining intensity of glandular, luminal, stromal, and muscular compartments for ERα in the uteri of rats was reduced in both T. pratense and estradiol treated animals compared to controls (Fig. 2A); however, this decrease was not statistically significant. PR staining intensity decreased in luminal and glandular compartments in T. pratense and estradiol treatment groups. No changes were observed in the staining intensity of muscular and stromal layers between the control and estradiol groups. Decreased stromal staining intensity was observed in the T. pratense group compared to the control group. No PR staining was observed in the muscular layer of T. pratense treated animals (Fig. 2B).


Evaluation of the estrogenic effects of dietary perinatal Trifolium pratense.

Yatkin E, Daglioglu S - J. Vet. Sci. (2011)

ERα (A) and PR (B) immunostaining score in the uterus of 21-day-old rats. The proportion of positively stained cells was scored as follows: 0, none; 1, weak; 2, moderate; 3, strong; 4, intense staining.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104165&req=5

Figure 2: ERα (A) and PR (B) immunostaining score in the uterus of 21-day-old rats. The proportion of positively stained cells was scored as follows: 0, none; 1, weak; 2, moderate; 3, strong; 4, intense staining.
Mentions: ERα and PR positive nuclei were localized in the epithelial, stromal, and muscular cells of the uteri (Fig. 1). Uterine sections not receiving primary antibody showed no staining. Strong PR immunoactivity was observed in the luminal epithelium of uterine tubes but only faint staining was observed in T. pratense and estradiol treated groups (Fig. 1). The staining intensity of glandular, luminal, stromal, and muscular compartments for ERα in the uteri of rats was reduced in both T. pratense and estradiol treated animals compared to controls (Fig. 2A); however, this decrease was not statistically significant. PR staining intensity decreased in luminal and glandular compartments in T. pratense and estradiol treatment groups. No changes were observed in the staining intensity of muscular and stromal layers between the control and estradiol groups. Decreased stromal staining intensity was observed in the T. pratense group compared to the control group. No PR staining was observed in the muscular layer of T. pratense treated animals (Fig. 2B).

Bottom Line: This study was designed to investigate the potential estrogenic effects of perinatal dietary phytoestrogens on the rat uterus.Our results revealed estrogen-like biological effects of perinatal T. pratense exposure.However, there were no statistically significant changes detected in the immunostaining intensity of ERα and PR between the groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey. emryat@gmail.com

ABSTRACT
This study was designed to investigate the potential estrogenic effects of perinatal dietary phytoestrogens on the rat uterus. Pregnant rats were divided to three groups provided the following diets: (1) rat chow, (2) rat chow with 7.5% Trifolium (T.) pratense, or (3) rat chow supplemented with 17β-estradiol (0.5 mg/kg). The dams in each group were kept on the same diet during pregnancy and lactation. Female offspring were euthanized on day 21 at which time body and organ weights were recorded and tissue samples were taken for histology. Immunohistochemistry was performed to detect estrogen receptor alpha (ERα) and progesterone receptor (PR) levels. Our results revealed estrogen-like biological effects of perinatal T. pratense exposure. Relative uterus and ovary weights in the experimental groups were increased compared to control. The number of uterine glands and luminal epithelium heights were also increased. However, there were no statistically significant changes detected in the immunostaining intensity of ERα and PR between the groups.

Show MeSH
Related in: MedlinePlus