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Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron.

Chang DW, Kim B, Shin JH, Yun YM, Je JH, Hwu YK, Yoon JH, Seong JK - J. Vet. Sci. (2011)

Bottom Line: At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron.Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography.Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

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Photographs of a mouse fetus thorax obtained by synchrotron radiation at embryonic day (E) 14 (A), E15 (B), and E18 (C, D). At E18, fetal ribs (arrows) and thoracic vertebra (arrowhead) were observed. Fetal ribs were also visualized at E14 and E15 but with lower definition.
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Figure 3: Photographs of a mouse fetus thorax obtained by synchrotron radiation at embryonic day (E) 14 (A), E15 (B), and E18 (C, D). At E18, fetal ribs (arrows) and thoracic vertebra (arrowhead) were observed. Fetal ribs were also visualized at E14 and E15 but with lower definition.

Mentions: Ten fetuses were visualized at E12, E14, E15, and E18 after gestation using a synchrotron radiation system. Fetal bones were not visualized in two mice at E12. Fig. 3A shows representative fetal ribs at E14; some portions of the ribs could not be viewed because they overlapped the mother's femur. Fetal bones were visualized in three out of four mice examined at E14 using the synchrotron radiation system. As shown in Fig. 3B, fetal ribs could be better observed (radiopaque) compared to E12. However, other bony structures were not visualized at this period by the synchrotron radiation system. Figs. 3C and D show that fetal ribs were more apparent and adjacent thoracic vertebrae were identified at E18. At E15 and E18, fetal bony structures were visualized in all mice examined (Table 1).


Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron.

Chang DW, Kim B, Shin JH, Yun YM, Je JH, Hwu YK, Yoon JH, Seong JK - J. Vet. Sci. (2011)

Photographs of a mouse fetus thorax obtained by synchrotron radiation at embryonic day (E) 14 (A), E15 (B), and E18 (C, D). At E18, fetal ribs (arrows) and thoracic vertebra (arrowhead) were observed. Fetal ribs were also visualized at E14 and E15 but with lower definition.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104163&req=5

Figure 3: Photographs of a mouse fetus thorax obtained by synchrotron radiation at embryonic day (E) 14 (A), E15 (B), and E18 (C, D). At E18, fetal ribs (arrows) and thoracic vertebra (arrowhead) were observed. Fetal ribs were also visualized at E14 and E15 but with lower definition.
Mentions: Ten fetuses were visualized at E12, E14, E15, and E18 after gestation using a synchrotron radiation system. Fetal bones were not visualized in two mice at E12. Fig. 3A shows representative fetal ribs at E14; some portions of the ribs could not be viewed because they overlapped the mother's femur. Fetal bones were visualized in three out of four mice examined at E14 using the synchrotron radiation system. As shown in Fig. 3B, fetal ribs could be better observed (radiopaque) compared to E12. However, other bony structures were not visualized at this period by the synchrotron radiation system. Figs. 3C and D show that fetal ribs were more apparent and adjacent thoracic vertebrae were identified at E18. At E15 and E18, fetal bony structures were visualized in all mice examined (Table 1).

Bottom Line: At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron.Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography.Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

Show MeSH
Related in: MedlinePlus