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Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron.

Chang DW, Kim B, Shin JH, Yun YM, Je JH, Hwu YK, Yoon JH, Seong JK - J. Vet. Sci. (2011)

Bottom Line: At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron.Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography.Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

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Related in: MedlinePlus

Photograph of a pregnant mouse suspended in the rectangular positioner with surgical ties. Synchrotron radiation imaging was performed with the mouse in an upright position.
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Figure 2: Photograph of a pregnant mouse suspended in the rectangular positioner with surgical ties. Synchrotron radiation imaging was performed with the mouse in an upright position.

Mentions: Mice were anesthetized with intraperitonial injections of ketamine (22.5 mg/kg; Yuhan, Korea) and xylazine (1.75 mg/kg; Bayer, Germany). Two mice at embryonic day (E)12, four mice at E14, two mice at E15, and two mice at E18 were radiographed with conventional X-ray (VPX-100A; Toshiba, Japan) and mammography equipment. To observe fetal anatomy, the pregnant mice were suspended around the rectangular positioner with surgical ties and placed in a vertical position (Fig. 2). The anesthetized mice were imaged with synchrotron radiation on the 5C1 beamline. Synchrotron radiation was derived from an accumulation ring of electrons with an accelerated energy of 2.5 Gev.


Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron.

Chang DW, Kim B, Shin JH, Yun YM, Je JH, Hwu YK, Yoon JH, Seong JK - J. Vet. Sci. (2011)

Photograph of a pregnant mouse suspended in the rectangular positioner with surgical ties. Synchrotron radiation imaging was performed with the mouse in an upright position.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104163&req=5

Figure 2: Photograph of a pregnant mouse suspended in the rectangular positioner with surgical ties. Synchrotron radiation imaging was performed with the mouse in an upright position.
Mentions: Mice were anesthetized with intraperitonial injections of ketamine (22.5 mg/kg; Yuhan, Korea) and xylazine (1.75 mg/kg; Bayer, Germany). Two mice at embryonic day (E)12, four mice at E14, two mice at E15, and two mice at E18 were radiographed with conventional X-ray (VPX-100A; Toshiba, Japan) and mammography equipment. To observe fetal anatomy, the pregnant mice were suspended around the rectangular positioner with surgical ties and placed in a vertical position (Fig. 2). The anesthetized mice were imaged with synchrotron radiation on the 5C1 beamline. Synchrotron radiation was derived from an accumulation ring of electrons with an accelerated energy of 2.5 Gev.

Bottom Line: At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron.Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography.Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.

ABSTRACT
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.

Show MeSH
Related in: MedlinePlus