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Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

Król J, Bania J, Florek M, Podkowik M, Pliszczak-Król A, Staroniewicz Z - Curr. Microbiol. (2011)

Bottom Line: A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison.Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375, Wroclaw, Poland. jkrol4@o2.pl

ABSTRACT
A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

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Related in: MedlinePlus

Agarose gel electrophoresis of a 476-bp fragment of the P. dagmatis 16S rRNA gene. Lanes 1–8 represent amplicons obtained from DNA of canine isolates, lanes 9–16 from DNA of feline isolates, and lanes 17–18 from DNA of the isolate from a tiger. For each isolate, intact amplicons (lanes with odd numbers) and TaqI-digested ones (lanes with even numbers) were run. M size marker (GeneRuler™100 bp DNA Ladder [Fermentas])
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Fig3: Agarose gel electrophoresis of a 476-bp fragment of the P. dagmatis 16S rRNA gene. Lanes 1–8 represent amplicons obtained from DNA of canine isolates, lanes 9–16 from DNA of feline isolates, and lanes 17–18 from DNA of the isolate from a tiger. For each isolate, intact amplicons (lanes with odd numbers) and TaqI-digested ones (lanes with even numbers) were run. M size marker (GeneRuler™100 bp DNA Ladder [Fermentas])

Mentions: The restriction nuclease TaqI was shown to cleave 476-bp fragments of the 16S rRNA gene from canine P. dagmatis isolates, giving products of 125 and 351 bp. Amplicons obtained from DNA of feline isolates (and the strain from a tiger) remained undigested (Fig. 3).Fig. 3


Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

Król J, Bania J, Florek M, Podkowik M, Pliszczak-Król A, Staroniewicz Z - Curr. Microbiol. (2011)

Agarose gel electrophoresis of a 476-bp fragment of the P. dagmatis 16S rRNA gene. Lanes 1–8 represent amplicons obtained from DNA of canine isolates, lanes 9–16 from DNA of feline isolates, and lanes 17–18 from DNA of the isolate from a tiger. For each isolate, intact amplicons (lanes with odd numbers) and TaqI-digested ones (lanes with even numbers) were run. M size marker (GeneRuler™100 bp DNA Ladder [Fermentas])
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104006&req=5

Fig3: Agarose gel electrophoresis of a 476-bp fragment of the P. dagmatis 16S rRNA gene. Lanes 1–8 represent amplicons obtained from DNA of canine isolates, lanes 9–16 from DNA of feline isolates, and lanes 17–18 from DNA of the isolate from a tiger. For each isolate, intact amplicons (lanes with odd numbers) and TaqI-digested ones (lanes with even numbers) were run. M size marker (GeneRuler™100 bp DNA Ladder [Fermentas])
Mentions: The restriction nuclease TaqI was shown to cleave 476-bp fragments of the 16S rRNA gene from canine P. dagmatis isolates, giving products of 125 and 351 bp. Amplicons obtained from DNA of feline isolates (and the strain from a tiger) remained undigested (Fig. 3).Fig. 3

Bottom Line: A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison.Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375, Wroclaw, Poland. jkrol4@o2.pl

ABSTRACT
A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

Show MeSH
Related in: MedlinePlus