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Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

Król J, Bania J, Florek M, Podkowik M, Pliszczak-Król A, Staroniewicz Z - Curr. Microbiol. (2011)

Bottom Line: A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison.Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375, Wroclaw, Poland. jkrol4@o2.pl

ABSTRACT
A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

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Phylogenetic tree of 28 Pasteurella strains based on rpoB sequences (501 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
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Fig2: Phylogenetic tree of 28 Pasteurella strains based on rpoB sequences (501 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger

Mentions: The rpoB gene tree (Fig. 2) was strongly supported by high bootstrap values (higher than 80% accounted for 12 out of 17 nodes). In this dendrogram, canine and feline isolates of P. dagmatis also clustered in separated groups. However, unlike the phylogeny derived from the 16S rRNA gene, an analysis of the rpoB sequences revealed that both canine and feline subpopulations of this species are more closely related to each other than to other members of the Pasteurella sensu stricto core group. The isolate from a tiger constituted a distinct P. dagmatis-like taxon, less closely related to the above P. dagmatis subgroups. The remaining rpoB gene sequences analyzed in this study were grouped in separate taxon-specific clusters. In both 16S rRNA and rpoB evolutionary trees, strains of P. pneumotropica (other than NCTC10827) constituted a group clearly different from feline isolates of P. dagmatis.Fig. 2


Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

Król J, Bania J, Florek M, Podkowik M, Pliszczak-Król A, Staroniewicz Z - Curr. Microbiol. (2011)

Phylogenetic tree of 28 Pasteurella strains based on rpoB sequences (501 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104006&req=5

Fig2: Phylogenetic tree of 28 Pasteurella strains based on rpoB sequences (501 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
Mentions: The rpoB gene tree (Fig. 2) was strongly supported by high bootstrap values (higher than 80% accounted for 12 out of 17 nodes). In this dendrogram, canine and feline isolates of P. dagmatis also clustered in separated groups. However, unlike the phylogeny derived from the 16S rRNA gene, an analysis of the rpoB sequences revealed that both canine and feline subpopulations of this species are more closely related to each other than to other members of the Pasteurella sensu stricto core group. The isolate from a tiger constituted a distinct P. dagmatis-like taxon, less closely related to the above P. dagmatis subgroups. The remaining rpoB gene sequences analyzed in this study were grouped in separate taxon-specific clusters. In both 16S rRNA and rpoB evolutionary trees, strains of P. pneumotropica (other than NCTC10827) constituted a group clearly different from feline isolates of P. dagmatis.Fig. 2

Bottom Line: A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison.Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375, Wroclaw, Poland. jkrol4@o2.pl

ABSTRACT
A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

Show MeSH
Related in: MedlinePlus