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Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

Król J, Bania J, Florek M, Podkowik M, Pliszczak-Król A, Staroniewicz Z - Curr. Microbiol. (2011)

Bottom Line: A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison.Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375, Wroclaw, Poland. jkrol4@o2.pl

ABSTRACT
A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

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Phylogenetic tree of 36 Pasteurella strains based on 16S rRNA sequences (1308 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; MCCM Medical Culture Collection Marburg, Germany; ATCC American Type Culture Collection, USA; CNP Centre National des Pasteurella, France; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
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Fig1: Phylogenetic tree of 36 Pasteurella strains based on 16S rRNA sequences (1308 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; MCCM Medical Culture Collection Marburg, Germany; ATCC American Type Culture Collection, USA; CNP Centre National des Pasteurella, France; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger

Mentions: A phylogenetic analysis of 16S rRNA sequences was performed with 36 sequences, including 16 isolates determined in the present study and 20 sequences deposited in GenBank (14 sequences of Pasteurella sensu stricto core group, four of P. pneumotropica, and two of Bisgaard Taxon 46). An evolutionary tree constructed by the neighbor-joining method was shown on Fig. 1. Of the 19 nodes, 15 were also supported by bootstrap values higher than 50%. Analyzed sequences were divided into nine clusters. Cluster IA was composed of the P. pneumotropica strain NCTC10827, two strains recovered from cats, designated as P. dagmatis-like 5/8 and 8/4 (sequences retrieved from GenBank), and 11 feline P. dagmatis isolates analyzed in this study. Members of this group displayed only a 97.48–97.60% sequence similarity with the P. dagmatis type strain (AY362920). Cluster IB contained the strain 197 from a tiger and the feline P. dagmatis-like strain 1/1 (from GenBank). Pasteurella stomatis and P. canis constituted clusters II and III, respectively. Four canine P. dagmatis isolates, characterized by the present authors, as well as the type strain of this species (CCUG12397T, sequence obtained from GenBank), fell into cluster IV. Clusters V, VI, and VII were composed of Bisgaard Taxon 46, P. multocida subsp. septica, and P. multocida subsp. multocida/P. multocida subsp. gallicida complex, respectively. Three sequences of P. pneumotropica (other than AF224296) were grouped in separate clusters VIII and IX with low similarity to all other sequences of the Pasteurella sensu stricto core group.Fig. 1


Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

Król J, Bania J, Florek M, Podkowik M, Pliszczak-Król A, Staroniewicz Z - Curr. Microbiol. (2011)

Phylogenetic tree of 36 Pasteurella strains based on 16S rRNA sequences (1308 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; MCCM Medical Culture Collection Marburg, Germany; ATCC American Type Culture Collection, USA; CNP Centre National des Pasteurella, France; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104006&req=5

Fig1: Phylogenetic tree of 36 Pasteurella strains based on 16S rRNA sequences (1308 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; MCCM Medical Culture Collection Marburg, Germany; ATCC American Type Culture Collection, USA; CNP Centre National des Pasteurella, France; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
Mentions: A phylogenetic analysis of 16S rRNA sequences was performed with 36 sequences, including 16 isolates determined in the present study and 20 sequences deposited in GenBank (14 sequences of Pasteurella sensu stricto core group, four of P. pneumotropica, and two of Bisgaard Taxon 46). An evolutionary tree constructed by the neighbor-joining method was shown on Fig. 1. Of the 19 nodes, 15 were also supported by bootstrap values higher than 50%. Analyzed sequences were divided into nine clusters. Cluster IA was composed of the P. pneumotropica strain NCTC10827, two strains recovered from cats, designated as P. dagmatis-like 5/8 and 8/4 (sequences retrieved from GenBank), and 11 feline P. dagmatis isolates analyzed in this study. Members of this group displayed only a 97.48–97.60% sequence similarity with the P. dagmatis type strain (AY362920). Cluster IB contained the strain 197 from a tiger and the feline P. dagmatis-like strain 1/1 (from GenBank). Pasteurella stomatis and P. canis constituted clusters II and III, respectively. Four canine P. dagmatis isolates, characterized by the present authors, as well as the type strain of this species (CCUG12397T, sequence obtained from GenBank), fell into cluster IV. Clusters V, VI, and VII were composed of Bisgaard Taxon 46, P. multocida subsp. septica, and P. multocida subsp. multocida/P. multocida subsp. gallicida complex, respectively. Three sequences of P. pneumotropica (other than AF224296) were grouped in separate clusters VIII and IX with low similarity to all other sequences of the Pasteurella sensu stricto core group.Fig. 1

Bottom Line: A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison.Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375, Wroclaw, Poland. jkrol4@o2.pl

ABSTRACT
A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

Show MeSH
Related in: MedlinePlus