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Successful use of squeezed-fat grafts to correct a breast affected by Poland syndrome.

Yang H, Lee H - Aesthetic Plast Surg (2010)

Bottom Line: After surgery, several small cysts and minimal calcifications were present but no significant complications.In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications.The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

View Article: PubMed Central - PubMed

Affiliation: Kangnam Plastic Surgery, Shinsa-Dong 577-7, Kangnam-Gu, Seoul, South Korea.

ABSTRACT
This study attempted to reconstruct deformities of a Poland syndrome patient using autologous fat tissues. All injected fat tissues were condensed by squeezing centrifugation. Operations were performed four times with intervals over 6 months. The total injection volume was 972 ml, and the maintained volume of 628 ml was measured by means of a magnetic resonance image (MRI). The entire follow-up period was 4.5 years. After surgery, several small cysts and minimal calcifications were present but no significant complications. The cosmetic outcomes and volume maintenance rates were excellent despite the overlapped large-volume injections. In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications. It is necessary, however, to perform more comparative studies with many clinical cases for a more scientific analysis. The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

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Related in: MedlinePlus

Cell counts and viabilities in simple centrifuged fat and squeezed fat. a Numbers of viable cells in simple centrifuged fat and squeezed fat, respectively. b Numbers of cells in simple centrifuged fat and squeezed fat, respectively. c Cell viability in simple centrifuged fat and squeezed fat, respectively
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Fig7: Cell counts and viabilities in simple centrifuged fat and squeezed fat. a Numbers of viable cells in simple centrifuged fat and squeezed fat, respectively. b Numbers of cells in simple centrifuged fat and squeezed fat, respectively. c Cell viability in simple centrifuged fat and squeezed fat, respectively

Mentions: We used a 22-g piston plunger 2.6 cm in diameter and centrifuged at 3,000 g for 5 min. We were able to calculate the pressure within fat tissues during centrifugation by assessing the actual weight (0.022 kg × 3,000) and cut surface area (3.14 × 1.3 × 1.3 cm). The pressure within the fat tissue was 12.5 kg/cm2. We also tried to evaluate the harmful influences of this squeezing process by comparing the viabilities of 1 ml of crude fat and 1 ml of squeezed fat. The total number of ASCs increased in unit volume, but the viability comparison did not show significant differences (Fig. 7a–c). This finding implies that actual condensation occurred and that the squeezing process did not have significant harmful effects on ASC.Fig. 7


Successful use of squeezed-fat grafts to correct a breast affected by Poland syndrome.

Yang H, Lee H - Aesthetic Plast Surg (2010)

Cell counts and viabilities in simple centrifuged fat and squeezed fat. a Numbers of viable cells in simple centrifuged fat and squeezed fat, respectively. b Numbers of cells in simple centrifuged fat and squeezed fat, respectively. c Cell viability in simple centrifuged fat and squeezed fat, respectively
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104005&req=5

Fig7: Cell counts and viabilities in simple centrifuged fat and squeezed fat. a Numbers of viable cells in simple centrifuged fat and squeezed fat, respectively. b Numbers of cells in simple centrifuged fat and squeezed fat, respectively. c Cell viability in simple centrifuged fat and squeezed fat, respectively
Mentions: We used a 22-g piston plunger 2.6 cm in diameter and centrifuged at 3,000 g for 5 min. We were able to calculate the pressure within fat tissues during centrifugation by assessing the actual weight (0.022 kg × 3,000) and cut surface area (3.14 × 1.3 × 1.3 cm). The pressure within the fat tissue was 12.5 kg/cm2. We also tried to evaluate the harmful influences of this squeezing process by comparing the viabilities of 1 ml of crude fat and 1 ml of squeezed fat. The total number of ASCs increased in unit volume, but the viability comparison did not show significant differences (Fig. 7a–c). This finding implies that actual condensation occurred and that the squeezing process did not have significant harmful effects on ASC.Fig. 7

Bottom Line: After surgery, several small cysts and minimal calcifications were present but no significant complications.In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications.The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

View Article: PubMed Central - PubMed

Affiliation: Kangnam Plastic Surgery, Shinsa-Dong 577-7, Kangnam-Gu, Seoul, South Korea.

ABSTRACT
This study attempted to reconstruct deformities of a Poland syndrome patient using autologous fat tissues. All injected fat tissues were condensed by squeezing centrifugation. Operations were performed four times with intervals over 6 months. The total injection volume was 972 ml, and the maintained volume of 628 ml was measured by means of a magnetic resonance image (MRI). The entire follow-up period was 4.5 years. After surgery, several small cysts and minimal calcifications were present but no significant complications. The cosmetic outcomes and volume maintenance rates were excellent despite the overlapped large-volume injections. In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications. It is necessary, however, to perform more comparative studies with many clinical cases for a more scientific analysis. The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

Show MeSH
Related in: MedlinePlus