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Successful use of squeezed-fat grafts to correct a breast affected by Poland syndrome.

Yang H, Lee H - Aesthetic Plast Surg (2010)

Bottom Line: After surgery, several small cysts and minimal calcifications were present but no significant complications.In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications.The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

View Article: PubMed Central - PubMed

Affiliation: Kangnam Plastic Surgery, Shinsa-Dong 577-7, Kangnam-Gu, Seoul, South Korea.

ABSTRACT
This study attempted to reconstruct deformities of a Poland syndrome patient using autologous fat tissues. All injected fat tissues were condensed by squeezing centrifugation. Operations were performed four times with intervals over 6 months. The total injection volume was 972 ml, and the maintained volume of 628 ml was measured by means of a magnetic resonance image (MRI). The entire follow-up period was 4.5 years. After surgery, several small cysts and minimal calcifications were present but no significant complications. The cosmetic outcomes and volume maintenance rates were excellent despite the overlapped large-volume injections. In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications. It is necessary, however, to perform more comparative studies with many clinical cases for a more scientific analysis. The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

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Highly condensed fat tissues (“fat gel”) using sharp-blade medical homogenizer. a Morphology of fat gel made by the medical homogenizer (Filler-Geller; Medikan Co. Ltd., Seoul, Korea). b Gross morphology of fat gel. c Histologic analysis of fat gel. Hematoxylin and eosin (H&E) staining of fresh fat gel. Violet (nucleus). Pink (cytoplasm). White rectangle (location at which the stem cell is highly condensed). d Comparison of volume maintenance between squeezed fat and fat gel in nude mice after 4 weeks. e Cell-counting analysis comparing squeezed fat and fat gel. f Comparison of adipose-derived stem cell (ASC) viability between squeezed fat and fat gel
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Fig10: Highly condensed fat tissues (“fat gel”) using sharp-blade medical homogenizer. a Morphology of fat gel made by the medical homogenizer (Filler-Geller; Medikan Co. Ltd., Seoul, Korea). b Gross morphology of fat gel. c Histologic analysis of fat gel. Hematoxylin and eosin (H&E) staining of fresh fat gel. Violet (nucleus). Pink (cytoplasm). White rectangle (location at which the stem cell is highly condensed). d Comparison of volume maintenance between squeezed fat and fat gel in nude mice after 4 weeks. e Cell-counting analysis comparing squeezed fat and fat gel. f Comparison of adipose-derived stem cell (ASC) viability between squeezed fat and fat gel

Mentions: Fat tissues are composed not only of adipocytes but also of stem cells, scaffolds, and growth signals such as growth factors. We therefore have hypothesized that targets of condensation may not be adipocytes. In small-volume fat grafts, we often use gel-like fat tissues made by the sharp-blade homogenization process. We call this gel-like tissue, composed of micronized condensed fat tissues, “fat gel.” Highly condensed viable stem cells and collagen structures are abundant in these tissues. This finding supports our rationale that mechanical destruction may positively affect fat grafting (Fig. 10). We almost always use this type of condensed fat tissue for fine-finishing fat grafting to correct an incidental irregularity or areola defining because fat gel can be injected with thinner needles (e.g., 26 gauge). We can suggest this process as another type of mechanical destructing condensation, but this procedure also requires long-term follow-up assessment and discussion.Fig. 10


Successful use of squeezed-fat grafts to correct a breast affected by Poland syndrome.

Yang H, Lee H - Aesthetic Plast Surg (2010)

Highly condensed fat tissues (“fat gel”) using sharp-blade medical homogenizer. a Morphology of fat gel made by the medical homogenizer (Filler-Geller; Medikan Co. Ltd., Seoul, Korea). b Gross morphology of fat gel. c Histologic analysis of fat gel. Hematoxylin and eosin (H&E) staining of fresh fat gel. Violet (nucleus). Pink (cytoplasm). White rectangle (location at which the stem cell is highly condensed). d Comparison of volume maintenance between squeezed fat and fat gel in nude mice after 4 weeks. e Cell-counting analysis comparing squeezed fat and fat gel. f Comparison of adipose-derived stem cell (ASC) viability between squeezed fat and fat gel
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3104005&req=5

Fig10: Highly condensed fat tissues (“fat gel”) using sharp-blade medical homogenizer. a Morphology of fat gel made by the medical homogenizer (Filler-Geller; Medikan Co. Ltd., Seoul, Korea). b Gross morphology of fat gel. c Histologic analysis of fat gel. Hematoxylin and eosin (H&E) staining of fresh fat gel. Violet (nucleus). Pink (cytoplasm). White rectangle (location at which the stem cell is highly condensed). d Comparison of volume maintenance between squeezed fat and fat gel in nude mice after 4 weeks. e Cell-counting analysis comparing squeezed fat and fat gel. f Comparison of adipose-derived stem cell (ASC) viability between squeezed fat and fat gel
Mentions: Fat tissues are composed not only of adipocytes but also of stem cells, scaffolds, and growth signals such as growth factors. We therefore have hypothesized that targets of condensation may not be adipocytes. In small-volume fat grafts, we often use gel-like fat tissues made by the sharp-blade homogenization process. We call this gel-like tissue, composed of micronized condensed fat tissues, “fat gel.” Highly condensed viable stem cells and collagen structures are abundant in these tissues. This finding supports our rationale that mechanical destruction may positively affect fat grafting (Fig. 10). We almost always use this type of condensed fat tissue for fine-finishing fat grafting to correct an incidental irregularity or areola defining because fat gel can be injected with thinner needles (e.g., 26 gauge). We can suggest this process as another type of mechanical destructing condensation, but this procedure also requires long-term follow-up assessment and discussion.Fig. 10

Bottom Line: After surgery, several small cysts and minimal calcifications were present but no significant complications.In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications.The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

View Article: PubMed Central - PubMed

Affiliation: Kangnam Plastic Surgery, Shinsa-Dong 577-7, Kangnam-Gu, Seoul, South Korea.

ABSTRACT
This study attempted to reconstruct deformities of a Poland syndrome patient using autologous fat tissues. All injected fat tissues were condensed by squeezing centrifugation. Operations were performed four times with intervals over 6 months. The total injection volume was 972 ml, and the maintained volume of 628 ml was measured by means of a magnetic resonance image (MRI). The entire follow-up period was 4.5 years. After surgery, several small cysts and minimal calcifications were present but no significant complications. The cosmetic outcomes and volume maintenance rates were excellent despite the overlapped large-volume injections. In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications. It is necessary, however, to perform more comparative studies with many clinical cases for a more scientific analysis. The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.

Show MeSH
Related in: MedlinePlus