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Proteomic analysis of honeybee (Apis mellifera L.) pupae head development.

Zheng A, Li J, Begna D, Fang Y, Feng M, Song F - PLoS ONE (2011)

Bottom Line: Furthermore, the constructed protein interaction network predicted 33 proteins acting as key nodes of honeybee pupae head growth of which 9 and 4 proteins were validated at gene and protein levels, respectively.In this study, we uncovered potential protein species involved in the formation of honeybee pupae head development along with their specific temporal requirements.This first proteomic result allows deeper understanding of the proteome profile changes during honeybee pupae head development and provides important potential candidate proteins for future reverse genetic research on honeybee pupae head development to improve the performance of related organs.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture/Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing, China.

ABSTRACT
The honeybee pupae development influences its future adult condition as well as honey and royal jelly productions. However, the molecular mechanism that regulates honeybee pupae head metamorphosis is still poorly understood. To further our understand of the associated molecular mechanism, we investigated the protein change of the honeybee pupae head at 5 time-points using 2-D electrophoresis, mass spectrometry, bioinformatics, quantitative real-time polymerase chain reaction and Western blot analysis. Accordingly, 58 protein spots altered their expression across the 5 time points (13-20 days), of which 36 proteins involved in the head organogenesis were upregulated during early stages (13-17 days). However, 22 proteins involved in regulating the pupae head neuron and gland development were upregulated at later developmental stages (19-20 days). Also, the functional enrichment analysis further suggests that proteins related to carbohydrate metabolism and energy production, development, cytoskeleton and protein folding were highly involved in the generation of organs and development of honeybee pupal head. Furthermore, the constructed protein interaction network predicted 33 proteins acting as key nodes of honeybee pupae head growth of which 9 and 4 proteins were validated at gene and protein levels, respectively. In this study, we uncovered potential protein species involved in the formation of honeybee pupae head development along with their specific temporal requirements. This first proteomic result allows deeper understanding of the proteome profile changes during honeybee pupae head development and provides important potential candidate proteins for future reverse genetic research on honeybee pupae head development to improve the performance of related organs.

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Functional annotation and distributions of the differentially expressed proteins identified from honeybee pupae head at different developmental time points.
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pone-0020428-g004: Functional annotation and distributions of the differentially expressed proteins identified from honeybee pupae head at different developmental time points.

Mentions: The identified proteins were grouped into 11 functional classes and proteins involved in carbohydrates metabolism and energy production, development, cytoskeleton, protein folding and protein biosythesis were found to be the major protein families (Fig. 4). Interestingly, the proportions of functional classes (except antioxidant and fatty acid metabolism proteins) indicated higher representation from early to middle than the late developmental stages. Specifically, nucleotide and amino acid metabolisms proteins were expressed only during the early to mid developmental stages (Fig. 5).


Proteomic analysis of honeybee (Apis mellifera L.) pupae head development.

Zheng A, Li J, Begna D, Fang Y, Feng M, Song F - PLoS ONE (2011)

Functional annotation and distributions of the differentially expressed proteins identified from honeybee pupae head at different developmental time points.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3102718&req=5

pone-0020428-g004: Functional annotation and distributions of the differentially expressed proteins identified from honeybee pupae head at different developmental time points.
Mentions: The identified proteins were grouped into 11 functional classes and proteins involved in carbohydrates metabolism and energy production, development, cytoskeleton, protein folding and protein biosythesis were found to be the major protein families (Fig. 4). Interestingly, the proportions of functional classes (except antioxidant and fatty acid metabolism proteins) indicated higher representation from early to middle than the late developmental stages. Specifically, nucleotide and amino acid metabolisms proteins were expressed only during the early to mid developmental stages (Fig. 5).

Bottom Line: Furthermore, the constructed protein interaction network predicted 33 proteins acting as key nodes of honeybee pupae head growth of which 9 and 4 proteins were validated at gene and protein levels, respectively.In this study, we uncovered potential protein species involved in the formation of honeybee pupae head development along with their specific temporal requirements.This first proteomic result allows deeper understanding of the proteome profile changes during honeybee pupae head development and provides important potential candidate proteins for future reverse genetic research on honeybee pupae head development to improve the performance of related organs.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture/Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing, China.

ABSTRACT
The honeybee pupae development influences its future adult condition as well as honey and royal jelly productions. However, the molecular mechanism that regulates honeybee pupae head metamorphosis is still poorly understood. To further our understand of the associated molecular mechanism, we investigated the protein change of the honeybee pupae head at 5 time-points using 2-D electrophoresis, mass spectrometry, bioinformatics, quantitative real-time polymerase chain reaction and Western blot analysis. Accordingly, 58 protein spots altered their expression across the 5 time points (13-20 days), of which 36 proteins involved in the head organogenesis were upregulated during early stages (13-17 days). However, 22 proteins involved in regulating the pupae head neuron and gland development were upregulated at later developmental stages (19-20 days). Also, the functional enrichment analysis further suggests that proteins related to carbohydrate metabolism and energy production, development, cytoskeleton and protein folding were highly involved in the generation of organs and development of honeybee pupal head. Furthermore, the constructed protein interaction network predicted 33 proteins acting as key nodes of honeybee pupae head growth of which 9 and 4 proteins were validated at gene and protein levels, respectively. In this study, we uncovered potential protein species involved in the formation of honeybee pupae head development along with their specific temporal requirements. This first proteomic result allows deeper understanding of the proteome profile changes during honeybee pupae head development and provides important potential candidate proteins for future reverse genetic research on honeybee pupae head development to improve the performance of related organs.

Show MeSH
Related in: MedlinePlus