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Drosophila eggshell production: identification of new genes and coordination by Pxt.

Tootle TL, Williams D, Hubb A, Frederick R, Spradling A - PLoS ONE (2011)

Bottom Line: Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects.One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression.These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Roy J. and Lucille Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America. tina-tootle@uiowa.edu

ABSTRACT
Drosophila ovarian follicles complete development using a spatially and temporally controlled maturation process in which they resume meiosis and secrete a multi-layered, protective eggshell before undergoing arrest and/or ovulation. Microarray analysis revealed more than 150 genes that are expressed in a stage-specific manner during the last 24 hours of follicle development. These include all 30 previously known eggshell genes, as well as 19 new candidate chorion genes and 100 other genes likely to participate in maturation. Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects. Somatic activity of Pxt is required, as RNAi knockdown of pxt in the follicle cells recapitulates both the temporal expression and eggshell defects. One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression. These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals.

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Expression of new putative eggshell protein and late follicle transcripts.(A) The temporal expression patterns of 19 new putative eggshell protein transcripts as determined by microarray analyses. The full dynamic range cannot be depicted and the scale differs from Figure 1. Numerical values can be found in Table S2. Adjacent genes with the same color are clustered in the genome. (B) The temporal expression patterns of 32 genes in various categories from among the differentially expressed genes identified (for the full list see Table S3). Transposon = Tirant (1640955_s_at). Accession numbers are listed in Tables S2, S3.
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pone-0019943-g002: Expression of new putative eggshell protein and late follicle transcripts.(A) The temporal expression patterns of 19 new putative eggshell protein transcripts as determined by microarray analyses. The full dynamic range cannot be depicted and the scale differs from Figure 1. Numerical values can be found in Table S2. Adjacent genes with the same color are clustered in the genome. (B) The temporal expression patterns of 32 genes in various categories from among the differentially expressed genes identified (for the full list see Table S3). Transposon = Tirant (1640955_s_at). Accession numbers are listed in Tables S2, S3.

Mentions: The results observed with the 30 genes encoding known eggshell proteins suggested that this approach was both accurate and highly sensitive (Figure 1E). Transcripts from all eggshell genes were detected and their stage-specificities of expression were accurately recapitulated (Table S1), validating the reliability of our experimental paradigm. In addition, 120 additional genes were found whose transcripts also accumulated in a stage-specific pattern (Figure 2, Tables S2, S3). We verified the expression behavior of a subset of these new genes using RT-PCR (Figure S1).


Drosophila eggshell production: identification of new genes and coordination by Pxt.

Tootle TL, Williams D, Hubb A, Frederick R, Spradling A - PLoS ONE (2011)

Expression of new putative eggshell protein and late follicle transcripts.(A) The temporal expression patterns of 19 new putative eggshell protein transcripts as determined by microarray analyses. The full dynamic range cannot be depicted and the scale differs from Figure 1. Numerical values can be found in Table S2. Adjacent genes with the same color are clustered in the genome. (B) The temporal expression patterns of 32 genes in various categories from among the differentially expressed genes identified (for the full list see Table S3). Transposon = Tirant (1640955_s_at). Accession numbers are listed in Tables S2, S3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3102670&req=5

pone-0019943-g002: Expression of new putative eggshell protein and late follicle transcripts.(A) The temporal expression patterns of 19 new putative eggshell protein transcripts as determined by microarray analyses. The full dynamic range cannot be depicted and the scale differs from Figure 1. Numerical values can be found in Table S2. Adjacent genes with the same color are clustered in the genome. (B) The temporal expression patterns of 32 genes in various categories from among the differentially expressed genes identified (for the full list see Table S3). Transposon = Tirant (1640955_s_at). Accession numbers are listed in Tables S2, S3.
Mentions: The results observed with the 30 genes encoding known eggshell proteins suggested that this approach was both accurate and highly sensitive (Figure 1E). Transcripts from all eggshell genes were detected and their stage-specificities of expression were accurately recapitulated (Table S1), validating the reliability of our experimental paradigm. In addition, 120 additional genes were found whose transcripts also accumulated in a stage-specific pattern (Figure 2, Tables S2, S3). We verified the expression behavior of a subset of these new genes using RT-PCR (Figure S1).

Bottom Line: Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects.One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression.These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Roy J. and Lucille Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America. tina-tootle@uiowa.edu

ABSTRACT
Drosophila ovarian follicles complete development using a spatially and temporally controlled maturation process in which they resume meiosis and secrete a multi-layered, protective eggshell before undergoing arrest and/or ovulation. Microarray analysis revealed more than 150 genes that are expressed in a stage-specific manner during the last 24 hours of follicle development. These include all 30 previously known eggshell genes, as well as 19 new candidate chorion genes and 100 other genes likely to participate in maturation. Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects. Somatic activity of Pxt is required, as RNAi knockdown of pxt in the follicle cells recapitulates both the temporal expression and eggshell defects. One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression. These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals.

Show MeSH