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Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals.

Ueno-Yamanouchi A, Khan FM, Serushago B, Bowen T, Lu C, Luider J, Storek J - Allergy Asthma Clin Immunol (2011)

Bottom Line: Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology & Laboratory Medicine, University of Calgary, Room 269, Heritage Medical Research Building, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. fkhan@ucalgary.ca.

ABSTRACT

Background: Allergen-specific IgE production is a hallmark of allergic asthma/rhinitis/eczema. Theoretically this could be due to a high number of allergen-specific B cells or allergen-specific T cells helping allergen-specific B cells differentiate into IgE-producing plasma cells. Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.

Methods: A total of 52 allergic individuals and 32 nonallergic individuals were studied. The allergen-specific B and Th cells were enumerated by culturing CFSE-loaded blood mononuclear cells for 7-days with allergen (cat, Timothy or birch), and determining the number of proliferating B or Th cells (diluting CFSE) by flow cytometry. Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).

Results: The quantities of proliferating Th cells but not proliferating B cells specific for cat, Timothy and birch were significantly higher in cat-, Timothy- and birch-allergic individuals compared to nonallergic individuals. The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

Conclusions: A high number of allergen-specific proliferating Th cells, but not proliferating B cells, may play a role in the pathogenesis of allergic asthma/rhinitis/eczema.

No MeSH data available.


Related in: MedlinePlus

Ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells (left) and ratio of positive control (anti CD3)-specific B cells and allergen-specific B cells (right) in allergic patients (n = 33, closed diamonds) and nonallergic persons (n = 18, open diamonds). The numbers of allergic patients were 18 for cat, 23 for Timothy, and 19 for birch. Significance of the difference between the allergic and nonallergic groups is given in the upper section of each plot. The horizontal bars show the medians.
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Figure 5: Ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells (left) and ratio of positive control (anti CD3)-specific B cells and allergen-specific B cells (right) in allergic patients (n = 33, closed diamonds) and nonallergic persons (n = 18, open diamonds). The numbers of allergic patients were 18 for cat, 23 for Timothy, and 19 for birch. Significance of the difference between the allergic and nonallergic groups is given in the upper section of each plot. The horizontal bars show the medians.

Mentions: Analogous to the indices, the absolute counts of allergen-specific B cells were similar in individuals allergic to any of the allergens analyzed compared to nonallergic individuals (Figure 4, left). Also analogous to the indices, the absolute counts of allergen-specific Th cells were significantly higher in individuals allergic to cat, Timothy or birch compared to nonallergic individuals (Figure 4, right). We then compared the ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells to rule out the impact of run variability. Similar to the absolute counts of allergen-specific Th cells, the ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells was significantly higher in individuals allergic to cat, Timothy or birch compared to nonallergic individuals (Figure 5). The absolute counts of Th and B cells were found similar in allergic individuals allergic to one and more than one allergen.


Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals.

Ueno-Yamanouchi A, Khan FM, Serushago B, Bowen T, Lu C, Luider J, Storek J - Allergy Asthma Clin Immunol (2011)

Ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells (left) and ratio of positive control (anti CD3)-specific B cells and allergen-specific B cells (right) in allergic patients (n = 33, closed diamonds) and nonallergic persons (n = 18, open diamonds). The numbers of allergic patients were 18 for cat, 23 for Timothy, and 19 for birch. Significance of the difference between the allergic and nonallergic groups is given in the upper section of each plot. The horizontal bars show the medians.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3102632&req=5

Figure 5: Ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells (left) and ratio of positive control (anti CD3)-specific B cells and allergen-specific B cells (right) in allergic patients (n = 33, closed diamonds) and nonallergic persons (n = 18, open diamonds). The numbers of allergic patients were 18 for cat, 23 for Timothy, and 19 for birch. Significance of the difference between the allergic and nonallergic groups is given in the upper section of each plot. The horizontal bars show the medians.
Mentions: Analogous to the indices, the absolute counts of allergen-specific B cells were similar in individuals allergic to any of the allergens analyzed compared to nonallergic individuals (Figure 4, left). Also analogous to the indices, the absolute counts of allergen-specific Th cells were significantly higher in individuals allergic to cat, Timothy or birch compared to nonallergic individuals (Figure 4, right). We then compared the ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells to rule out the impact of run variability. Similar to the absolute counts of allergen-specific Th cells, the ratio of positive control (anti CD3)-specific Th cell and allergen-specific Th cells was significantly higher in individuals allergic to cat, Timothy or birch compared to nonallergic individuals (Figure 5). The absolute counts of Th and B cells were found similar in allergic individuals allergic to one and more than one allergen.

Bottom Line: Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology & Laboratory Medicine, University of Calgary, Room 269, Heritage Medical Research Building, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. fkhan@ucalgary.ca.

ABSTRACT

Background: Allergen-specific IgE production is a hallmark of allergic asthma/rhinitis/eczema. Theoretically this could be due to a high number of allergen-specific B cells or allergen-specific T cells helping allergen-specific B cells differentiate into IgE-producing plasma cells. Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.

Methods: A total of 52 allergic individuals and 32 nonallergic individuals were studied. The allergen-specific B and Th cells were enumerated by culturing CFSE-loaded blood mononuclear cells for 7-days with allergen (cat, Timothy or birch), and determining the number of proliferating B or Th cells (diluting CFSE) by flow cytometry. Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).

Results: The quantities of proliferating Th cells but not proliferating B cells specific for cat, Timothy and birch were significantly higher in cat-, Timothy- and birch-allergic individuals compared to nonallergic individuals. The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

Conclusions: A high number of allergen-specific proliferating Th cells, but not proliferating B cells, may play a role in the pathogenesis of allergic asthma/rhinitis/eczema.

No MeSH data available.


Related in: MedlinePlus