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Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals.

Ueno-Yamanouchi A, Khan FM, Serushago B, Bowen T, Lu C, Luider J, Storek J - Allergy Asthma Clin Immunol (2011)

Bottom Line: Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology & Laboratory Medicine, University of Calgary, Room 269, Heritage Medical Research Building, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. fkhan@ucalgary.ca.

ABSTRACT

Background: Allergen-specific IgE production is a hallmark of allergic asthma/rhinitis/eczema. Theoretically this could be due to a high number of allergen-specific B cells or allergen-specific T cells helping allergen-specific B cells differentiate into IgE-producing plasma cells. Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.

Methods: A total of 52 allergic individuals and 32 nonallergic individuals were studied. The allergen-specific B and Th cells were enumerated by culturing CFSE-loaded blood mononuclear cells for 7-days with allergen (cat, Timothy or birch), and determining the number of proliferating B or Th cells (diluting CFSE) by flow cytometry. Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).

Results: The quantities of proliferating Th cells but not proliferating B cells specific for cat, Timothy and birch were significantly higher in cat-, Timothy- and birch-allergic individuals compared to nonallergic individuals. The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

Conclusions: A high number of allergen-specific proliferating Th cells, but not proliferating B cells, may play a role in the pathogenesis of allergic asthma/rhinitis/eczema.

No MeSH data available.


Related in: MedlinePlus

Example of calculation of the index of the quantity of allergen-specific Th cells and the number of precursor cells of acquired allergen-specific cells (needed to calculate the absolute count of allergen specific Th cells). The percentage of the CFSElow cells after the 7 day culture is referred to as the "index" of the quantity of allergen-specific cells (in this example, Timothy-specific Th index). The number of precursor cells of acquired allergen-specific cells (in this example, precursor cells of CFSElow Th cells), determined using the ModFit software, and was used to estimate the absolute count of allergen-specific Th cells. Only generations 3 and higher were considered as allergen-specific cells and generations 0, 1 and 2 were omitted from the calculation.
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Figure 2: Example of calculation of the index of the quantity of allergen-specific Th cells and the number of precursor cells of acquired allergen-specific cells (needed to calculate the absolute count of allergen specific Th cells). The percentage of the CFSElow cells after the 7 day culture is referred to as the "index" of the quantity of allergen-specific cells (in this example, Timothy-specific Th index). The number of precursor cells of acquired allergen-specific cells (in this example, precursor cells of CFSElow Th cells), determined using the ModFit software, and was used to estimate the absolute count of allergen-specific Th cells. Only generations 3 and higher were considered as allergen-specific cells and generations 0, 1 and 2 were omitted from the calculation.

Mentions: The method of calculation of index and absolute count of allergen specific B, Th, Th1 and Th2 cells is displayed in Figure 2. The percentage of the CFSElow cells on day 7 of culture is referred to as the "index" of the quantity of allergen-specific cells. The absolute count of the allergen-specific cells was determined from the absolute MNC count on day 0 (absolute lymphocyte count + absolute monocyte count per ml of blood), the acquired cell proportion on day 7 (determined as the acquired proportion of fluorospheres, eg, 0.8 if 40,000 of the 50,000 fluorospheres were acquired), and the number of precursor cells of acquired (by flow cytometry) allergen-specific cells (determined using Modfit software, Verity Software House, see next paragraph for details). The absolute count of allergen-specific cells (per ml of blood) was calculated using the following formula:


Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals.

Ueno-Yamanouchi A, Khan FM, Serushago B, Bowen T, Lu C, Luider J, Storek J - Allergy Asthma Clin Immunol (2011)

Example of calculation of the index of the quantity of allergen-specific Th cells and the number of precursor cells of acquired allergen-specific cells (needed to calculate the absolute count of allergen specific Th cells). The percentage of the CFSElow cells after the 7 day culture is referred to as the "index" of the quantity of allergen-specific cells (in this example, Timothy-specific Th index). The number of precursor cells of acquired allergen-specific cells (in this example, precursor cells of CFSElow Th cells), determined using the ModFit software, and was used to estimate the absolute count of allergen-specific Th cells. Only generations 3 and higher were considered as allergen-specific cells and generations 0, 1 and 2 were omitted from the calculation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3102632&req=5

Figure 2: Example of calculation of the index of the quantity of allergen-specific Th cells and the number of precursor cells of acquired allergen-specific cells (needed to calculate the absolute count of allergen specific Th cells). The percentage of the CFSElow cells after the 7 day culture is referred to as the "index" of the quantity of allergen-specific cells (in this example, Timothy-specific Th index). The number of precursor cells of acquired allergen-specific cells (in this example, precursor cells of CFSElow Th cells), determined using the ModFit software, and was used to estimate the absolute count of allergen-specific Th cells. Only generations 3 and higher were considered as allergen-specific cells and generations 0, 1 and 2 were omitted from the calculation.
Mentions: The method of calculation of index and absolute count of allergen specific B, Th, Th1 and Th2 cells is displayed in Figure 2. The percentage of the CFSElow cells on day 7 of culture is referred to as the "index" of the quantity of allergen-specific cells. The absolute count of the allergen-specific cells was determined from the absolute MNC count on day 0 (absolute lymphocyte count + absolute monocyte count per ml of blood), the acquired cell proportion on day 7 (determined as the acquired proportion of fluorospheres, eg, 0.8 if 40,000 of the 50,000 fluorospheres were acquired), and the number of precursor cells of acquired (by flow cytometry) allergen-specific cells (determined using Modfit software, Verity Software House, see next paragraph for details). The absolute count of allergen-specific cells (per ml of blood) was calculated using the following formula:

Bottom Line: Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology & Laboratory Medicine, University of Calgary, Room 269, Heritage Medical Research Building, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. fkhan@ucalgary.ca.

ABSTRACT

Background: Allergen-specific IgE production is a hallmark of allergic asthma/rhinitis/eczema. Theoretically this could be due to a high number of allergen-specific B cells or allergen-specific T cells helping allergen-specific B cells differentiate into IgE-producing plasma cells. Here, we determined whether the number of allergen-specific B cells or T helper (Th) cells is higher in allergic individuals compared to nonallergic individuals.

Methods: A total of 52 allergic individuals and 32 nonallergic individuals were studied. The allergen-specific B and Th cells were enumerated by culturing CFSE-loaded blood mononuclear cells for 7-days with allergen (cat, Timothy or birch), and determining the number of proliferating B or Th cells (diluting CFSE) by flow cytometry. Allergen-specific IgE concentration was determined by fluorescent enzymoimmunoassay (FEIA).

Results: The quantities of proliferating Th cells but not proliferating B cells specific for cat, Timothy and birch were significantly higher in cat-, Timothy- and birch-allergic individuals compared to nonallergic individuals. The titer of allergen-specific IgE showed significant correlation with allergen-specific Th cells and not with allergen-specific B cells for all 3 allergens.

Conclusions: A high number of allergen-specific proliferating Th cells, but not proliferating B cells, may play a role in the pathogenesis of allergic asthma/rhinitis/eczema.

No MeSH data available.


Related in: MedlinePlus