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Comparison of TCP and TCP/HA Hybrid Scaffolds for Osteoconductive Activity.

Wongwitwichot P, Kaewsrichan J, Chua KH, Ruszymah BH - Open Biomed Eng J (2010)

Bottom Line: The result showed decreasing of the strength by HA supplement, suggesting the more brittle characteristic of HA in comparison with TCP.Such change was found to be favorable for cell attachment, migration, and growth.Thus, the composite scaffold was found superior for hard tissue regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.

ABSTRACT
Two types of porous ceramic scaffolds were prepared, consisting of β-tricalcium phosphate (TCP) or the mixed powder of TCP and hydroxyapatite (HA) at a 2:1 mass ratio. A variety of methods have been used to fabricate bone scaffolds, while the sintering approach was adopted in this work. An extremely high temperature was used on sintering that proposed to consolidate the ceramic particles. As revealed by SEM, a well opened pore structure was developed within the scaffolds. The θ-values were measured to be of 73.3° and 6.5° for the composite scaffold and TCP sample, respectively. According to XRD patterns, the existence of grains coalescence and partial bonding between HA and TCP powders was demonstrated. Scaffold mechanical property in the term of flexural strength was also determined. The result showed decreasing of the strength by HA supplement, suggesting the more brittle characteristic of HA in comparison with TCP. By soaking the composite scaffold in PBS for a period of 2 weeks, transformation from particles to flank-like crystalline was clearly observed. Such change was found to be favorable for cell attachment, migration, and growth. By implanting cell-seeded scaffolds into nude mice, an abundant osseous extracellular matrix was identified for the composite implants. In contrast, the matrix was minimally detected in TCP implanted samples. Thus, the composite scaffold was found superior for hard tissue regeneration.

No MeSH data available.


Related in: MedlinePlus

SEM images of the fabricated scaffolds (A and B) and the commercial product from BD Bioscience (C). A: HT63, composed the combination of HA and TCP at a 2:1 mass ratio; B: T80, prepared by using pure TCP; C: the scaffold called BD, being the calcium phosphate based structure obtained from BD Bioscience.
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Figure 3: SEM images of the fabricated scaffolds (A and B) and the commercial product from BD Bioscience (C). A: HT63, composed the combination of HA and TCP at a 2:1 mass ratio; B: T80, prepared by using pure TCP; C: the scaffold called BD, being the calcium phosphate based structure obtained from BD Bioscience.

Mentions: SEM images of the prepared scaffolds and the commercial product were demonstrated in Fig. (3). An opened pore structure was developed in both types of the fabricated scaffolds (3A and 3B). A denser microstructure with smaller pore size (15 ± 3 µm) was revealed by HT63 composites, whereas larger pores of 43 ± 8 µm diameter were formed in T80 scaffolds. According to “The product manual” of BD Bioscience, the pore diameter of BD scaffolds ranged between 200 and 400 µm (Fig. 3C). A homogeneous phase inside the composite scaffolds (HT63) was observed, but grain boundary was generally apparent in TCP samples (T80). Thus, the pore structure was changed by adding HA into TCP scaffold base. The removal of polyvinyl alcohol at high temperatures provided interconnected porous structure with porosity of approximately 40%, as demonstrated in Fig. (3A and 3B).


Comparison of TCP and TCP/HA Hybrid Scaffolds for Osteoconductive Activity.

Wongwitwichot P, Kaewsrichan J, Chua KH, Ruszymah BH - Open Biomed Eng J (2010)

SEM images of the fabricated scaffolds (A and B) and the commercial product from BD Bioscience (C). A: HT63, composed the combination of HA and TCP at a 2:1 mass ratio; B: T80, prepared by using pure TCP; C: the scaffold called BD, being the calcium phosphate based structure obtained from BD Bioscience.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3102423&req=5

Figure 3: SEM images of the fabricated scaffolds (A and B) and the commercial product from BD Bioscience (C). A: HT63, composed the combination of HA and TCP at a 2:1 mass ratio; B: T80, prepared by using pure TCP; C: the scaffold called BD, being the calcium phosphate based structure obtained from BD Bioscience.
Mentions: SEM images of the prepared scaffolds and the commercial product were demonstrated in Fig. (3). An opened pore structure was developed in both types of the fabricated scaffolds (3A and 3B). A denser microstructure with smaller pore size (15 ± 3 µm) was revealed by HT63 composites, whereas larger pores of 43 ± 8 µm diameter were formed in T80 scaffolds. According to “The product manual” of BD Bioscience, the pore diameter of BD scaffolds ranged between 200 and 400 µm (Fig. 3C). A homogeneous phase inside the composite scaffolds (HT63) was observed, but grain boundary was generally apparent in TCP samples (T80). Thus, the pore structure was changed by adding HA into TCP scaffold base. The removal of polyvinyl alcohol at high temperatures provided interconnected porous structure with porosity of approximately 40%, as demonstrated in Fig. (3A and 3B).

Bottom Line: The result showed decreasing of the strength by HA supplement, suggesting the more brittle characteristic of HA in comparison with TCP.Such change was found to be favorable for cell attachment, migration, and growth.Thus, the composite scaffold was found superior for hard tissue regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.

ABSTRACT
Two types of porous ceramic scaffolds were prepared, consisting of β-tricalcium phosphate (TCP) or the mixed powder of TCP and hydroxyapatite (HA) at a 2:1 mass ratio. A variety of methods have been used to fabricate bone scaffolds, while the sintering approach was adopted in this work. An extremely high temperature was used on sintering that proposed to consolidate the ceramic particles. As revealed by SEM, a well opened pore structure was developed within the scaffolds. The θ-values were measured to be of 73.3° and 6.5° for the composite scaffold and TCP sample, respectively. According to XRD patterns, the existence of grains coalescence and partial bonding between HA and TCP powders was demonstrated. Scaffold mechanical property in the term of flexural strength was also determined. The result showed decreasing of the strength by HA supplement, suggesting the more brittle characteristic of HA in comparison with TCP. By soaking the composite scaffold in PBS for a period of 2 weeks, transformation from particles to flank-like crystalline was clearly observed. Such change was found to be favorable for cell attachment, migration, and growth. By implanting cell-seeded scaffolds into nude mice, an abundant osseous extracellular matrix was identified for the composite implants. In contrast, the matrix was minimally detected in TCP implanted samples. Thus, the composite scaffold was found superior for hard tissue regeneration.

No MeSH data available.


Related in: MedlinePlus