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Hydroxyapatite Mineralization on the Calcium Chloride Blended Polyurethane Nanofiber via Biomimetic Method.

Nirmala R, Nam KT, Navamathavan R, Park SJ, Kim HY - Nanoscale Res Lett (2010)

Bottom Line: SEM images revealed that the CaCl2 salt incorporated homogeneously to form well-oriented nanofibers with smooth surface and uniform diameters along their lengths.The SBF incubation test confirmed the formation of apatite-like materials, exhibiting enhanced bioactive behavior of the polyurethane/CaCl2 composite nanofibers.This study demonstrated that the electrospun polyurethane containing CaCl2 composite nanofibers enhanced the in vitro bioactivity and supports the growth of apatite-like materials.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Healthcare Technology and Development, Chonbuk National University, Jeonju 561 756, South Korea. khy@jbnu.ac.kr.

ABSTRACT
Polyurethane nanofibers containing calcium chloride (CaCl2) were prepared via an electrospinning technique for the biomedical applications. Polyurethane nanofibers with different concentration of CaCl2 were electrospun, and their bioactivity evaluation was conducted by incubating in biomimetic simulated body fluid (SBF) solution. The morphology, structure and thermal properties of the polyurethane/CaCl2 composite nanofibers were characterized by means of scanning electron microscopy (SEM), field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy and thermogravimetry. SEM images revealed that the CaCl2 salt incorporated homogeneously to form well-oriented nanofibers with smooth surface and uniform diameters along their lengths. The SBF incubation test confirmed the formation of apatite-like materials, exhibiting enhanced bioactive behavior of the polyurethane/CaCl2 composite nanofibers. This study demonstrated that the electrospun polyurethane containing CaCl2 composite nanofibers enhanced the in vitro bioactivity and supports the growth of apatite-like materials.

No MeSH data available.


High magnification FE-SEM images of electrospun polyurethane nanofibers containing different concentration of CaCl2 salt before incubation in SBF solution (a1) 0, (b1) 1, (c1) 1.5 and (d1) 2 wt% and the corresponding EDX spectra (a2)–(d2), respectively.
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Figure 1: High magnification FE-SEM images of electrospun polyurethane nanofibers containing different concentration of CaCl2 salt before incubation in SBF solution (a1) 0, (b1) 1, (c1) 1.5 and (d1) 2 wt% and the corresponding EDX spectra (a2)–(d2), respectively.

Mentions: The CaCl2 salt was mixed in the polyurethane polymer solution and then fabricated as composite nanofibers. Figure 1 shows the FE-SEM images and EDX spectra of the electrospun polyurethane nanofibers containing different concentration of CaCl2 salt with 0, 1, 1.5 and 2 wt%. These as-spun polyurethane nanofibers exhibited a smooth surface and uniform diameters along their lengths. By varying the experimental parameters such as, applied voltage, syringe micro-tip distance, solvent concentration, amount of salt content and flow rate, one can obtain highly uniform ultrafine nanofibers with different pore sizes. As shown in Figures 1b12d1, mesh-like ultrafine nanofibers were formed when we added the CaCl2 salt in the polymer solution. This kind of phenomenon is in good agreement with those of our previous reports [21], which can be utilized for the biomedical applications as the bone regenerative materials. As expected, the addition of CaCl2 can decrease the fiber diameter and also forming ultrafine mesh–shaped nanofibers in between the main fibers. The density of these mesh-like nanofibers was increased monotonically with increasing CaCl2 concentration from 1 to 2 wt%. The diameter of these ultrafine nanofibers (~8–20 nm) was of one order lower than those of the main fibers (~200–400 nm), which resulted in a large surface area-to-volume ratio and interconnected porosity. These features are considered to be of particular interest as they mimic the extracellular matrix as present in the natural tissue and have been shown to induce a significant increase in protein absorption and cell adhesion, compared to their micro-size counterparts [20]. In this study, in order to investigate the effect of CaCl2 on the formation of apatite nucleation in the SBF solution, different concentrations of CaCl2 incorporated in the polyurethane nanofibers were synthesized.


Hydroxyapatite Mineralization on the Calcium Chloride Blended Polyurethane Nanofiber via Biomimetic Method.

Nirmala R, Nam KT, Navamathavan R, Park SJ, Kim HY - Nanoscale Res Lett (2010)

High magnification FE-SEM images of electrospun polyurethane nanofibers containing different concentration of CaCl2 salt before incubation in SBF solution (a1) 0, (b1) 1, (c1) 1.5 and (d1) 2 wt% and the corresponding EDX spectra (a2)–(d2), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3102341&req=5

Figure 1: High magnification FE-SEM images of electrospun polyurethane nanofibers containing different concentration of CaCl2 salt before incubation in SBF solution (a1) 0, (b1) 1, (c1) 1.5 and (d1) 2 wt% and the corresponding EDX spectra (a2)–(d2), respectively.
Mentions: The CaCl2 salt was mixed in the polyurethane polymer solution and then fabricated as composite nanofibers. Figure 1 shows the FE-SEM images and EDX spectra of the electrospun polyurethane nanofibers containing different concentration of CaCl2 salt with 0, 1, 1.5 and 2 wt%. These as-spun polyurethane nanofibers exhibited a smooth surface and uniform diameters along their lengths. By varying the experimental parameters such as, applied voltage, syringe micro-tip distance, solvent concentration, amount of salt content and flow rate, one can obtain highly uniform ultrafine nanofibers with different pore sizes. As shown in Figures 1b12d1, mesh-like ultrafine nanofibers were formed when we added the CaCl2 salt in the polymer solution. This kind of phenomenon is in good agreement with those of our previous reports [21], which can be utilized for the biomedical applications as the bone regenerative materials. As expected, the addition of CaCl2 can decrease the fiber diameter and also forming ultrafine mesh–shaped nanofibers in between the main fibers. The density of these mesh-like nanofibers was increased monotonically with increasing CaCl2 concentration from 1 to 2 wt%. The diameter of these ultrafine nanofibers (~8–20 nm) was of one order lower than those of the main fibers (~200–400 nm), which resulted in a large surface area-to-volume ratio and interconnected porosity. These features are considered to be of particular interest as they mimic the extracellular matrix as present in the natural tissue and have been shown to induce a significant increase in protein absorption and cell adhesion, compared to their micro-size counterparts [20]. In this study, in order to investigate the effect of CaCl2 on the formation of apatite nucleation in the SBF solution, different concentrations of CaCl2 incorporated in the polyurethane nanofibers were synthesized.

Bottom Line: SEM images revealed that the CaCl2 salt incorporated homogeneously to form well-oriented nanofibers with smooth surface and uniform diameters along their lengths.The SBF incubation test confirmed the formation of apatite-like materials, exhibiting enhanced bioactive behavior of the polyurethane/CaCl2 composite nanofibers.This study demonstrated that the electrospun polyurethane containing CaCl2 composite nanofibers enhanced the in vitro bioactivity and supports the growth of apatite-like materials.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Healthcare Technology and Development, Chonbuk National University, Jeonju 561 756, South Korea. khy@jbnu.ac.kr.

ABSTRACT
Polyurethane nanofibers containing calcium chloride (CaCl2) were prepared via an electrospinning technique for the biomedical applications. Polyurethane nanofibers with different concentration of CaCl2 were electrospun, and their bioactivity evaluation was conducted by incubating in biomimetic simulated body fluid (SBF) solution. The morphology, structure and thermal properties of the polyurethane/CaCl2 composite nanofibers were characterized by means of scanning electron microscopy (SEM), field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy and thermogravimetry. SEM images revealed that the CaCl2 salt incorporated homogeneously to form well-oriented nanofibers with smooth surface and uniform diameters along their lengths. The SBF incubation test confirmed the formation of apatite-like materials, exhibiting enhanced bioactive behavior of the polyurethane/CaCl2 composite nanofibers. This study demonstrated that the electrospun polyurethane containing CaCl2 composite nanofibers enhanced the in vitro bioactivity and supports the growth of apatite-like materials.

No MeSH data available.