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Adipocytokines and CD34 progenitor cells in Alzheimer's disease.

Bigalke B, Schreitmüller B, Sopova K, Paul A, Stransky E, Gawaz M, Stellos K, Laske C - PLoS ONE (2011)

Bottom Line: We found significantly lower plasma levels of leptin in AD patients compared with the controls, whereas plasma levels of adiponectin did not show any significant differences (AD vs. control (mean ± SD): leptin:8.9 ± 5.6 ng/mL vs.16.3 ± 15.5 ng/mL;P = 0.038; adiponectin:18.5 ± 18.1 µg/mL vs.16.7 ± 8.9 µg/mL;P = 0.641).In logistic regression analysis, decreased leptin concentration (P = 0.021) and increased number of CD34(+) cells (P = 0.036) were both significantly associated with the presence of AD.In addition, the results of our study provide first evidence that increased leptin plasma levels are associated with a reduced number of CD34(+) progenitor cells in AD patients.

View Article: PubMed Central - PubMed

Affiliation: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Tübingen, Germany.

ABSTRACT

Background: Alzheimer's disease (AD) and atherosclerosis share common vascular risk factors such as arterial hypertension and hypercholesterolemia. Adipocytokines and CD34(+) progenitor cells are associated with the progression and prognosis of atherosclerotic diseases. Their role in AD is not adequately elucidated.

Methods and findings: In the present study, we measured in 41 patients with early AD and 37 age- and weight-matched healthy controls blood concentrations of adiponectin and leptin by enzyme linked immunoabsorbent assay and of CD34(+) progenitor cells using flow cytometry. We found significantly lower plasma levels of leptin in AD patients compared with the controls, whereas plasma levels of adiponectin did not show any significant differences (AD vs. control (mean ± SD): leptin:8.9 ± 5.6 ng/mL vs.16.3 ± 15.5 ng/mL;P = 0.038; adiponectin:18.5 ± 18.1 µg/mL vs.16.7 ± 8.9 µg/mL;P = 0.641). In contrast, circulating CD34(+) cells were significantly upregulated in AD patients (mean absolute cell count ± SD:253 ± 51 vs. 203 ± 37; P = 0.02) and showed an inverse correlation with plasma levels of leptin (r =  -0.248; P = 0.037). In logistic regression analysis, decreased leptin concentration (P = 0.021) and increased number of CD34(+) cells (P = 0.036) were both significantly associated with the presence of AD. According to multifactorial analysis of covariance, leptin serum levels were a significant independent predictor for the number of CD34(+) cells (P = 0.002).

Conclusions: Our findings suggest that low plasma levels of leptin and increased numbers of CD34(+) progenitor cells are both associated with AD. In addition, the results of our study provide first evidence that increased leptin plasma levels are associated with a reduced number of CD34(+) progenitor cells in AD patients. These findings point towards a combined involvement of leptin and CD34(+) progenitor cells in the pathogenesis of AD. Thus, plasma levels of leptin and circulating CD34(+) progenitor cells could represent an important molecular link between atherosclerotic diseases and AD. Further studies should clarify the pathophysiological role of both adipocytokines and progenitor cells in AD and possible diagnostic and therapeutic applications.

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Plasma levels of adipocytokines leptin and adiponectin, and number of                            CD34+ progenitor cells in Alzheimer's disease                        (AD).(A) AD patients showed significantly lower plasma leptin levels compared with                        healthy controls (P = 0.038). (B) Plasma levels of                        adiponectin did not show any significant differences between AD patients and                        healthy controls (P = 0.641). (C) Number of                            CD34+ progenitor cells [mean absolute cell                        count] was significantly upregulated in patients with AD compared to                        control (P = 0.02). (D) Plasma levels of leptin                        inversely correlated with the number of CD34+ progenitor                        cells (r = −0.248;                        P = 0.037).
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pone-0020286-g001: Plasma levels of adipocytokines leptin and adiponectin, and number of CD34+ progenitor cells in Alzheimer's disease (AD).(A) AD patients showed significantly lower plasma leptin levels compared with healthy controls (P = 0.038). (B) Plasma levels of adiponectin did not show any significant differences between AD patients and healthy controls (P = 0.641). (C) Number of CD34+ progenitor cells [mean absolute cell count] was significantly upregulated in patients with AD compared to control (P = 0.02). (D) Plasma levels of leptin inversely correlated with the number of CD34+ progenitor cells (r = −0.248; P = 0.037).

Mentions: We found significantly lower plasma levels of leptin in AD patients compared with healthy controls (Fig. 1A), whereas plasma levels of adiponectin did not show any significant differences (AD vs. control (mean±SD): leptin: 8.9±5.6 ng/mL vs. 16.3±15.5 ng/mL; P = 0.038; adiponectin: 18.5±18.1 µg/mL vs. 16.7±8.9 µg/mL; P = 0.641) (Fig. 1B). However, plasma levels of both adipocytokines significantly correlated with each other (r = 0.402; P = 0.001) in the combined sample pool of AD patients and controls.


Adipocytokines and CD34 progenitor cells in Alzheimer's disease.

Bigalke B, Schreitmüller B, Sopova K, Paul A, Stransky E, Gawaz M, Stellos K, Laske C - PLoS ONE (2011)

Plasma levels of adipocytokines leptin and adiponectin, and number of                            CD34+ progenitor cells in Alzheimer's disease                        (AD).(A) AD patients showed significantly lower plasma leptin levels compared with                        healthy controls (P = 0.038). (B) Plasma levels of                        adiponectin did not show any significant differences between AD patients and                        healthy controls (P = 0.641). (C) Number of                            CD34+ progenitor cells [mean absolute cell                        count] was significantly upregulated in patients with AD compared to                        control (P = 0.02). (D) Plasma levels of leptin                        inversely correlated with the number of CD34+ progenitor                        cells (r = −0.248;                        P = 0.037).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3102092&req=5

pone-0020286-g001: Plasma levels of adipocytokines leptin and adiponectin, and number of CD34+ progenitor cells in Alzheimer's disease (AD).(A) AD patients showed significantly lower plasma leptin levels compared with healthy controls (P = 0.038). (B) Plasma levels of adiponectin did not show any significant differences between AD patients and healthy controls (P = 0.641). (C) Number of CD34+ progenitor cells [mean absolute cell count] was significantly upregulated in patients with AD compared to control (P = 0.02). (D) Plasma levels of leptin inversely correlated with the number of CD34+ progenitor cells (r = −0.248; P = 0.037).
Mentions: We found significantly lower plasma levels of leptin in AD patients compared with healthy controls (Fig. 1A), whereas plasma levels of adiponectin did not show any significant differences (AD vs. control (mean±SD): leptin: 8.9±5.6 ng/mL vs. 16.3±15.5 ng/mL; P = 0.038; adiponectin: 18.5±18.1 µg/mL vs. 16.7±8.9 µg/mL; P = 0.641) (Fig. 1B). However, plasma levels of both adipocytokines significantly correlated with each other (r = 0.402; P = 0.001) in the combined sample pool of AD patients and controls.

Bottom Line: We found significantly lower plasma levels of leptin in AD patients compared with the controls, whereas plasma levels of adiponectin did not show any significant differences (AD vs. control (mean ± SD): leptin:8.9 ± 5.6 ng/mL vs.16.3 ± 15.5 ng/mL;P = 0.038; adiponectin:18.5 ± 18.1 µg/mL vs.16.7 ± 8.9 µg/mL;P = 0.641).In logistic regression analysis, decreased leptin concentration (P = 0.021) and increased number of CD34(+) cells (P = 0.036) were both significantly associated with the presence of AD.In addition, the results of our study provide first evidence that increased leptin plasma levels are associated with a reduced number of CD34(+) progenitor cells in AD patients.

View Article: PubMed Central - PubMed

Affiliation: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Tübingen, Germany.

ABSTRACT

Background: Alzheimer's disease (AD) and atherosclerosis share common vascular risk factors such as arterial hypertension and hypercholesterolemia. Adipocytokines and CD34(+) progenitor cells are associated with the progression and prognosis of atherosclerotic diseases. Their role in AD is not adequately elucidated.

Methods and findings: In the present study, we measured in 41 patients with early AD and 37 age- and weight-matched healthy controls blood concentrations of adiponectin and leptin by enzyme linked immunoabsorbent assay and of CD34(+) progenitor cells using flow cytometry. We found significantly lower plasma levels of leptin in AD patients compared with the controls, whereas plasma levels of adiponectin did not show any significant differences (AD vs. control (mean ± SD): leptin:8.9 ± 5.6 ng/mL vs.16.3 ± 15.5 ng/mL;P = 0.038; adiponectin:18.5 ± 18.1 µg/mL vs.16.7 ± 8.9 µg/mL;P = 0.641). In contrast, circulating CD34(+) cells were significantly upregulated in AD patients (mean absolute cell count ± SD:253 ± 51 vs. 203 ± 37; P = 0.02) and showed an inverse correlation with plasma levels of leptin (r =  -0.248; P = 0.037). In logistic regression analysis, decreased leptin concentration (P = 0.021) and increased number of CD34(+) cells (P = 0.036) were both significantly associated with the presence of AD. According to multifactorial analysis of covariance, leptin serum levels were a significant independent predictor for the number of CD34(+) cells (P = 0.002).

Conclusions: Our findings suggest that low plasma levels of leptin and increased numbers of CD34(+) progenitor cells are both associated with AD. In addition, the results of our study provide first evidence that increased leptin plasma levels are associated with a reduced number of CD34(+) progenitor cells in AD patients. These findings point towards a combined involvement of leptin and CD34(+) progenitor cells in the pathogenesis of AD. Thus, plasma levels of leptin and circulating CD34(+) progenitor cells could represent an important molecular link between atherosclerotic diseases and AD. Further studies should clarify the pathophysiological role of both adipocytokines and progenitor cells in AD and possible diagnostic and therapeutic applications.

Show MeSH
Related in: MedlinePlus