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Melatonin induces follicle maturation in Danio rerio.

Carnevali O, Gioacchini G, Maradonna F, Olivotto I, Migliarini B - PLoS ONE (2011)

Bottom Line: This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin.The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15.The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze del Mare, Università Politecnica delle Marche, Ancona, Italy. o.carnevali@univpm.it

ABSTRACT
Most organisms modulate their reproductive activity responding to day length by the nocturnal release of melatonin by the pineal gland. This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin. Melatonin led to an increase of the Gonado Somatic Index (GSI) associated with the increase of eggs production, and the raise of gene and protein levels of vitellogenin (VTG) and estradiol receptor α (ERα) in the liver. The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15. The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation. Melatonin also exerted a direct action on follicles as shown by the increase of the oocytes undergoing to germinal vesicle break down (GVBD) and modulated mpr α and β gene expression in the in vitro exposure.These data highlight the effects of melatonin in promoting zebrafish reproduction exerting its effects either in the brain-pituitary and in the gonads.

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Effects of melatonin in the brain and pituitary.(A) kiss1, (B) kiss2 (C) gnrh3 and (D) lh mRNA levels, normalized against β-actin gene in fish exposed to 100 nM and 1 µM MEL, n = 15 per group. Error bars indicate mean±S.D. Asterisks denote exposure groups that are significantly different from the relative control group (CTRL) (p<0.05), analyzed using ANOVA followed by Bonferroni's multiple comparison test.
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pone-0019978-g001: Effects of melatonin in the brain and pituitary.(A) kiss1, (B) kiss2 (C) gnrh3 and (D) lh mRNA levels, normalized against β-actin gene in fish exposed to 100 nM and 1 µM MEL, n = 15 per group. Error bars indicate mean±S.D. Asterisks denote exposure groups that are significantly different from the relative control group (CTRL) (p<0.05), analyzed using ANOVA followed by Bonferroni's multiple comparison test.

Mentions: The endocrine control of reproduction by exogenous melatonin was demonstrated by the modulation of the hypothalamic genes involved. Melatonin administration significantly increased, in a dose related manner, the gnrh3, kiss 1 and kiss 2 gene transcription, as reported in Fig. 1. The lh gene transcription was increased by both melatonin doses but only the higher ones induced a significant increase (Fig. 1).


Melatonin induces follicle maturation in Danio rerio.

Carnevali O, Gioacchini G, Maradonna F, Olivotto I, Migliarini B - PLoS ONE (2011)

Effects of melatonin in the brain and pituitary.(A) kiss1, (B) kiss2 (C) gnrh3 and (D) lh mRNA levels, normalized against β-actin gene in fish exposed to 100 nM and 1 µM MEL, n = 15 per group. Error bars indicate mean±S.D. Asterisks denote exposure groups that are significantly different from the relative control group (CTRL) (p<0.05), analyzed using ANOVA followed by Bonferroni's multiple comparison test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3102064&req=5

pone-0019978-g001: Effects of melatonin in the brain and pituitary.(A) kiss1, (B) kiss2 (C) gnrh3 and (D) lh mRNA levels, normalized against β-actin gene in fish exposed to 100 nM and 1 µM MEL, n = 15 per group. Error bars indicate mean±S.D. Asterisks denote exposure groups that are significantly different from the relative control group (CTRL) (p<0.05), analyzed using ANOVA followed by Bonferroni's multiple comparison test.
Mentions: The endocrine control of reproduction by exogenous melatonin was demonstrated by the modulation of the hypothalamic genes involved. Melatonin administration significantly increased, in a dose related manner, the gnrh3, kiss 1 and kiss 2 gene transcription, as reported in Fig. 1. The lh gene transcription was increased by both melatonin doses but only the higher ones induced a significant increase (Fig. 1).

Bottom Line: This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin.The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15.The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze del Mare, Università Politecnica delle Marche, Ancona, Italy. o.carnevali@univpm.it

ABSTRACT
Most organisms modulate their reproductive activity responding to day length by the nocturnal release of melatonin by the pineal gland. This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin. Melatonin led to an increase of the Gonado Somatic Index (GSI) associated with the increase of eggs production, and the raise of gene and protein levels of vitellogenin (VTG) and estradiol receptor α (ERα) in the liver. The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15. The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation. Melatonin also exerted a direct action on follicles as shown by the increase of the oocytes undergoing to germinal vesicle break down (GVBD) and modulated mpr α and β gene expression in the in vitro exposure.These data highlight the effects of melatonin in promoting zebrafish reproduction exerting its effects either in the brain-pituitary and in the gonads.

Show MeSH
Related in: MedlinePlus