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Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models.

Rooban BN, Sasikala V, Sahasranamam V, Abraham A - Mol. Vis. (2011)

Bottom Line: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group.Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.

ABSTRACT

Purpose: Cataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and microscopic approaches to investigate the attenuation of selenite cataract by the flavonoids from Vitex negundo (FVN).

Methods: To demonstrate this attenuation, Sprague-Dawley rat pups were divided into control (G I), selenite induced (G II), and selenite + FVN treated (G III). Cataract was induced by single subcutaneous injection of sodium selenite (4 mg/Kg bodyweight) on the 10th day and FVN (1 mg/Kg bodyweight) administered intraperitoneally from the 8th to the 15th day.

Results: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group. Post translational modifications identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) and immunoblot analysis revealed the loss of cytoskeletal proteins in selenite induced group. Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.

Conclusions: The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

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Related in: MedlinePlus

Gel filtration profiles of crystallins. Elution profiles of water soluble proteins from the experimental groups using Sephacryl - 300HR size exclusion chromatography. A: Control, B: Selenite induced, C: Selenite + FVN treated. αH, αL, βH, βL, and γ represents the corresponding crystallins elution peaks. Data are representative of three such independent assays for three separate lens extracts.
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f2: Gel filtration profiles of crystallins. Elution profiles of water soluble proteins from the experimental groups using Sephacryl - 300HR size exclusion chromatography. A: Control, B: Selenite induced, C: Selenite + FVN treated. αH, αL, βH, βL, and γ represents the corresponding crystallins elution peaks. Data are representative of three such independent assays for three separate lens extracts.

Mentions: To investigate possible alterations in crystallins profile of selenite cataract and the influence of V. negundo, the soluble proteins were analyzed by Sephacryl - 300HR size exclusion chromatography. Data are representative of three such independent assays for three separate lens extracts. Analysis of crystallin profile suggests that the quality of the existing soluble proteins varied between Group II and Group I (Figure 2A,B). The elution profile of selenite induced group showed a slight increase of αH- and a decrease of αL-, βH-, and βL-crystallin peaks compared with control. However, there was no difference in the γ-crystallin peak in these groups (Figure 2A,B). Nevertheless, all these modifications were prevented by FVN treatment (Figure 2C).


Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models.

Rooban BN, Sasikala V, Sahasranamam V, Abraham A - Mol. Vis. (2011)

Gel filtration profiles of crystallins. Elution profiles of water soluble proteins from the experimental groups using Sephacryl - 300HR size exclusion chromatography. A: Control, B: Selenite induced, C: Selenite + FVN treated. αH, αL, βH, βL, and γ represents the corresponding crystallins elution peaks. Data are representative of three such independent assays for three separate lens extracts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3102022&req=5

f2: Gel filtration profiles of crystallins. Elution profiles of water soluble proteins from the experimental groups using Sephacryl - 300HR size exclusion chromatography. A: Control, B: Selenite induced, C: Selenite + FVN treated. αH, αL, βH, βL, and γ represents the corresponding crystallins elution peaks. Data are representative of three such independent assays for three separate lens extracts.
Mentions: To investigate possible alterations in crystallins profile of selenite cataract and the influence of V. negundo, the soluble proteins were analyzed by Sephacryl - 300HR size exclusion chromatography. Data are representative of three such independent assays for three separate lens extracts. Analysis of crystallin profile suggests that the quality of the existing soluble proteins varied between Group II and Group I (Figure 2A,B). The elution profile of selenite induced group showed a slight increase of αH- and a decrease of αL-, βH-, and βL-crystallin peaks compared with control. However, there was no difference in the γ-crystallin peak in these groups (Figure 2A,B). Nevertheless, all these modifications were prevented by FVN treatment (Figure 2C).

Bottom Line: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group.Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.

ABSTRACT

Purpose: Cataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and microscopic approaches to investigate the attenuation of selenite cataract by the flavonoids from Vitex negundo (FVN).

Methods: To demonstrate this attenuation, Sprague-Dawley rat pups were divided into control (G I), selenite induced (G II), and selenite + FVN treated (G III). Cataract was induced by single subcutaneous injection of sodium selenite (4 mg/Kg bodyweight) on the 10th day and FVN (1 mg/Kg bodyweight) administered intraperitoneally from the 8th to the 15th day.

Results: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group. Post translational modifications identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) and immunoblot analysis revealed the loss of cytoskeletal proteins in selenite induced group. Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.

Conclusions: The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

Show MeSH
Related in: MedlinePlus