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Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models.

Rooban BN, Sasikala V, Sahasranamam V, Abraham A - Mol. Vis. (2011)

Bottom Line: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group.Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.

ABSTRACT

Purpose: Cataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and microscopic approaches to investigate the attenuation of selenite cataract by the flavonoids from Vitex negundo (FVN).

Methods: To demonstrate this attenuation, Sprague-Dawley rat pups were divided into control (G I), selenite induced (G II), and selenite + FVN treated (G III). Cataract was induced by single subcutaneous injection of sodium selenite (4 mg/Kg bodyweight) on the 10th day and FVN (1 mg/Kg bodyweight) administered intraperitoneally from the 8th to the 15th day.

Results: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group. Post translational modifications identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) and immunoblot analysis revealed the loss of cytoskeletal proteins in selenite induced group. Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.

Conclusions: The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

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Soluble and insoluble proteins in lens. Values are expressed as mean (n=8) ±SD. Comparison between groups, different alphabets indicate significant difference at p<0.05. Groupings are G-I: Control, G-II: Selenite induced, G-III: Selenite + FVN treated.
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f1: Soluble and insoluble proteins in lens. Values are expressed as mean (n=8) ±SD. Comparison between groups, different alphabets indicate significant difference at p<0.05. Groupings are G-I: Control, G-II: Selenite induced, G-III: Selenite + FVN treated.

Mentions: Whatever is the underlying mechanism, alteration in protein profile and insolublization of soluble protein has been considered to be the ultimate factor in lens opacification. Therefore, we analyzed the soluble and insoluble protein content in all the three groups. In control rats, no significant changes were observed in the ratio of soluble to insoluble proteins (Figure 1). In contrast, the ratios of soluble to insoluble proteins were altered in selenite induced group. A significant decrease of soluble and increase of insoluble proteins were observed compared with control (Figure 1). No significant changes in the loss of soluble to Insoluble protein ratio were found in the FVN treated group compared with control (Figure 1).


Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models.

Rooban BN, Sasikala V, Sahasranamam V, Abraham A - Mol. Vis. (2011)

Soluble and insoluble proteins in lens. Values are expressed as mean (n=8) ±SD. Comparison between groups, different alphabets indicate significant difference at p<0.05. Groupings are G-I: Control, G-II: Selenite induced, G-III: Selenite + FVN treated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3102022&req=5

f1: Soluble and insoluble proteins in lens. Values are expressed as mean (n=8) ±SD. Comparison between groups, different alphabets indicate significant difference at p<0.05. Groupings are G-I: Control, G-II: Selenite induced, G-III: Selenite + FVN treated.
Mentions: Whatever is the underlying mechanism, alteration in protein profile and insolublization of soluble protein has been considered to be the ultimate factor in lens opacification. Therefore, we analyzed the soluble and insoluble protein content in all the three groups. In control rats, no significant changes were observed in the ratio of soluble to insoluble proteins (Figure 1). In contrast, the ratios of soluble to insoluble proteins were altered in selenite induced group. A significant decrease of soluble and increase of insoluble proteins were observed compared with control (Figure 1). No significant changes in the loss of soluble to Insoluble protein ratio were found in the FVN treated group compared with control (Figure 1).

Bottom Line: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group.Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.

ABSTRACT

Purpose: Cataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and microscopic approaches to investigate the attenuation of selenite cataract by the flavonoids from Vitex negundo (FVN).

Methods: To demonstrate this attenuation, Sprague-Dawley rat pups were divided into control (G I), selenite induced (G II), and selenite + FVN treated (G III). Cataract was induced by single subcutaneous injection of sodium selenite (4 mg/Kg bodyweight) on the 10th day and FVN (1 mg/Kg bodyweight) administered intraperitoneally from the 8th to the 15th day.

Results: Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group. Post translational modifications identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) and immunoblot analysis revealed the loss of cytoskeletal proteins in selenite induced group. Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.

Conclusions: The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.

Show MeSH
Related in: MedlinePlus