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Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells.

Wang Y, Luo XP, Lim CE, Wong WS, Zhong G - ISRN Obstet Gynecol (2010)

Bottom Line: However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P > .05) after 24 hours of treatment.On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P > .05).Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynaecology, Guangdong Women and Children's Hospital and Health Institute, Guangzhou, 510010, China.

ABSTRACT
Objective. To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1). Methods. TEV-1 cells were incubated with different doses of peptidoglycan. The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining. Results. TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane. Compared with the untreated control, TEV-1 cells incubated with 30 μg/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment (P < .01). However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P > .05) after 24 hours of treatment. On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P > .05). Conclusion. Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells. It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.

No MeSH data available.


The expression of TLR2 protein in TEV-1 cells (immunocytochemistry SP × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig2: The expression of TLR2 protein in TEV-1 cells (immunocytochemistry SP × 200).

Mentions: The first objective of this study was to determine the expression pattern of TLR2 in the human extravillous trophoblast cell line TEV-1 cells. As shown in Figure 2(a), TEV-1 cells showed positive immunoreactivity for TLR2, and the positive brown granules were mainly distributed in the cytoplasm and cell membrane. That means that TLR2 was localized both on the cell surface as well as intracellularly in TEV-1 cells.


Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells.

Wang Y, Luo XP, Lim CE, Wong WS, Zhong G - ISRN Obstet Gynecol (2010)

The expression of TLR2 protein in TEV-1 cells (immunocytochemistry SP × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3101954&req=5

fig2: The expression of TLR2 protein in TEV-1 cells (immunocytochemistry SP × 200).
Mentions: The first objective of this study was to determine the expression pattern of TLR2 in the human extravillous trophoblast cell line TEV-1 cells. As shown in Figure 2(a), TEV-1 cells showed positive immunoreactivity for TLR2, and the positive brown granules were mainly distributed in the cytoplasm and cell membrane. That means that TLR2 was localized both on the cell surface as well as intracellularly in TEV-1 cells.

Bottom Line: However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P > .05) after 24 hours of treatment.On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P > .05).Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynaecology, Guangdong Women and Children's Hospital and Health Institute, Guangzhou, 510010, China.

ABSTRACT
Objective. To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1). Methods. TEV-1 cells were incubated with different doses of peptidoglycan. The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining. Results. TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane. Compared with the untreated control, TEV-1 cells incubated with 30 μg/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment (P < .01). However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P > .05) after 24 hours of treatment. On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P > .05). Conclusion. Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells. It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.

No MeSH data available.