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The RUB Cage: Respiration-Ultrasonic Vocalizations-Behavior Acquisition Setup for Assessing Emotional Memory in Rats.

Hegoburu C, Shionoya K, Garcia S, Messaoudi B, Thévenet M, Mouly AM - Front Behav Neurosci (2011)

Bottom Line: In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations.Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states.The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

View Article: PubMed Central - PubMed

Affiliation: Team "Olfaction: From Coding to Memory", Lyon Neuroscience Research Center, INSERM U1028, CNRS UMR5292 Lyon, France.

ABSTRACT
In animals, emotional memory is classically assessed through pavlovian fear conditioning in which a neutral novel stimulus (conditioned stimulus) is paired with an aversive unconditioned stimulus. After conditioning, the conditioned stimulus elicits a fear response characterized by a wide range of behavioral and physiological responses. Despite the existence of this large repertoire of responses, freezing behavior is often the sole parameter used for quantifying fear response, thus limiting emotional memory appraisal to this unique index. Interestingly, respiratory changes and ultrasonic vocalizations (USV) can occur during fear response, yet very few studies investigated the link between these different parameters and freezing. The aim of the present study was to design an experimental setup allowing the simultaneous recording of respiration, USV, and behavior (RUB cage), and the offline synchronization of the collected data for fine-grain second by second analysis. The setup consisted of a customized plethysmograph for respiration monitoring, equipped with a microphone capturing USV, and with four video cameras for behavior recording. In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations. Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states. Then we monitored these parameters during contextual fear conditioning and showed that they bring complementary information about the animal's anxiety state and the strength of aversive memory. The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

No MeSH data available.


Related in: MedlinePlus

Effect of the introduction of a discrete stimulus (a new odor) on the time-course of (A) behavior and (B) respiration. The three parameters were recorded in odor animals (n = 6), during a 30-s time window centered on odor presentation, at three periods of the experimental session (beginning, middle, and end). #Global significant difference between periods, p < 0.001. *Significant difference with pre-odor baseline, p < 0.05.
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Figure 5: Effect of the introduction of a discrete stimulus (a new odor) on the time-course of (A) behavior and (B) respiration. The three parameters were recorded in odor animals (n = 6), during a 30-s time window centered on odor presentation, at three periods of the experimental session (beginning, middle, and end). #Global significant difference between periods, p < 0.001. *Significant difference with pre-odor baseline, p < 0.05.

Mentions: We investigated whether transient changes could be observed on the respiratory signal when a discrete event, here a new odor (20 s duration), was introduced repeatedly (odor group). This paradigm first induced increased arousal due to novel stimulus detection, followed by habituation and progressive instatement of sleep. Since no USV were observed in these conditions, we focused on sleep behavior and respiration. We used a 35-s time window centered on odor presentation, at different periods of the experimental session, i.e., beginning (first odor presentation), middle (after 5 odor presentations), and end (after 10 odor presentations). The data are reported on Figure 5.


The RUB Cage: Respiration-Ultrasonic Vocalizations-Behavior Acquisition Setup for Assessing Emotional Memory in Rats.

Hegoburu C, Shionoya K, Garcia S, Messaoudi B, Thévenet M, Mouly AM - Front Behav Neurosci (2011)

Effect of the introduction of a discrete stimulus (a new odor) on the time-course of (A) behavior and (B) respiration. The three parameters were recorded in odor animals (n = 6), during a 30-s time window centered on odor presentation, at three periods of the experimental session (beginning, middle, and end). #Global significant difference between periods, p < 0.001. *Significant difference with pre-odor baseline, p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3101376&req=5

Figure 5: Effect of the introduction of a discrete stimulus (a new odor) on the time-course of (A) behavior and (B) respiration. The three parameters were recorded in odor animals (n = 6), during a 30-s time window centered on odor presentation, at three periods of the experimental session (beginning, middle, and end). #Global significant difference between periods, p < 0.001. *Significant difference with pre-odor baseline, p < 0.05.
Mentions: We investigated whether transient changes could be observed on the respiratory signal when a discrete event, here a new odor (20 s duration), was introduced repeatedly (odor group). This paradigm first induced increased arousal due to novel stimulus detection, followed by habituation and progressive instatement of sleep. Since no USV were observed in these conditions, we focused on sleep behavior and respiration. We used a 35-s time window centered on odor presentation, at different periods of the experimental session, i.e., beginning (first odor presentation), middle (after 5 odor presentations), and end (after 10 odor presentations). The data are reported on Figure 5.

Bottom Line: In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations.Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states.The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

View Article: PubMed Central - PubMed

Affiliation: Team "Olfaction: From Coding to Memory", Lyon Neuroscience Research Center, INSERM U1028, CNRS UMR5292 Lyon, France.

ABSTRACT
In animals, emotional memory is classically assessed through pavlovian fear conditioning in which a neutral novel stimulus (conditioned stimulus) is paired with an aversive unconditioned stimulus. After conditioning, the conditioned stimulus elicits a fear response characterized by a wide range of behavioral and physiological responses. Despite the existence of this large repertoire of responses, freezing behavior is often the sole parameter used for quantifying fear response, thus limiting emotional memory appraisal to this unique index. Interestingly, respiratory changes and ultrasonic vocalizations (USV) can occur during fear response, yet very few studies investigated the link between these different parameters and freezing. The aim of the present study was to design an experimental setup allowing the simultaneous recording of respiration, USV, and behavior (RUB cage), and the offline synchronization of the collected data for fine-grain second by second analysis. The setup consisted of a customized plethysmograph for respiration monitoring, equipped with a microphone capturing USV, and with four video cameras for behavior recording. In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations. Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states. Then we monitored these parameters during contextual fear conditioning and showed that they bring complementary information about the animal's anxiety state and the strength of aversive memory. The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

No MeSH data available.


Related in: MedlinePlus