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The RUB Cage: Respiration-Ultrasonic Vocalizations-Behavior Acquisition Setup for Assessing Emotional Memory in Rats.

Hegoburu C, Shionoya K, Garcia S, Messaoudi B, Thévenet M, Mouly AM - Front Behav Neurosci (2011)

Bottom Line: In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations.Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states.The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

View Article: PubMed Central - PubMed

Affiliation: Team "Olfaction: From Coding to Memory", Lyon Neuroscience Research Center, INSERM U1028, CNRS UMR5292 Lyon, France.

ABSTRACT
In animals, emotional memory is classically assessed through pavlovian fear conditioning in which a neutral novel stimulus (conditioned stimulus) is paired with an aversive unconditioned stimulus. After conditioning, the conditioned stimulus elicits a fear response characterized by a wide range of behavioral and physiological responses. Despite the existence of this large repertoire of responses, freezing behavior is often the sole parameter used for quantifying fear response, thus limiting emotional memory appraisal to this unique index. Interestingly, respiratory changes and ultrasonic vocalizations (USV) can occur during fear response, yet very few studies investigated the link between these different parameters and freezing. The aim of the present study was to design an experimental setup allowing the simultaneous recording of respiration, USV, and behavior (RUB cage), and the offline synchronization of the collected data for fine-grain second by second analysis. The setup consisted of a customized plethysmograph for respiration monitoring, equipped with a microphone capturing USV, and with four video cameras for behavior recording. In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations. Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states. Then we monitored these parameters during contextual fear conditioning and showed that they bring complementary information about the animal's anxiety state and the strength of aversive memory. The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

No MeSH data available.


Related in: MedlinePlus

(A) The experimental cage consisted in a customized plethysmograph used to measure respiratory activity in behaving rats. It is composed of two parts: the animal and reference chambers both connected to a differential pressure transducer. The ceiling of the plethysmograph was equipped with a tower allowing the introduction of three Tygon tubing to deliver air and odorants. The bottom of the animal chamber was connected to a ventilation pump. An ultrasound microphone was inserted in the tower to monitor ultrasonic vocalizations (USV) emitted by the rats. The floor of the animal chamber has been equipped with a shock-floor allowing delivering scrambled foot-shock. (B) Individual example of raw respiratory signal. I, inspiration phase; E, expiration phase. (C) Individual example of ultrasonic vocalizations. Upper part: waveform of three successive USV. Lower part: corresponding frequency spectrogram.
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Figure 1: (A) The experimental cage consisted in a customized plethysmograph used to measure respiratory activity in behaving rats. It is composed of two parts: the animal and reference chambers both connected to a differential pressure transducer. The ceiling of the plethysmograph was equipped with a tower allowing the introduction of three Tygon tubing to deliver air and odorants. The bottom of the animal chamber was connected to a ventilation pump. An ultrasound microphone was inserted in the tower to monitor ultrasonic vocalizations (USV) emitted by the rats. The floor of the animal chamber has been equipped with a shock-floor allowing delivering scrambled foot-shock. (B) Individual example of raw respiratory signal. I, inspiration phase; E, expiration phase. (C) Individual example of ultrasonic vocalizations. Upper part: waveform of three successive USV. Lower part: corresponding frequency spectrogram.

Mentions: The apparatus consisted of a whole body customized plethysmograph (Figure 1A; diameter 20 cm, height 30 cm, EMKA technologies, France; see also Figure A1 in Appendix) placed in a home made sound-attenuating cage (L 60 cm, W 60 cm, H 70 cm). The plethysmograph was used to measure respiratory parameters in behaving animals. It is composed of two parts which come together to form two independent airtight chambers: the animal and reference chambers. The pressure change due to animal respiration was measured by a differential pressure transducer (Model dpt, EMKA Technologies, France) with one port exposed to the animal chamber and the other to the reference chamber.


The RUB Cage: Respiration-Ultrasonic Vocalizations-Behavior Acquisition Setup for Assessing Emotional Memory in Rats.

Hegoburu C, Shionoya K, Garcia S, Messaoudi B, Thévenet M, Mouly AM - Front Behav Neurosci (2011)

(A) The experimental cage consisted in a customized plethysmograph used to measure respiratory activity in behaving rats. It is composed of two parts: the animal and reference chambers both connected to a differential pressure transducer. The ceiling of the plethysmograph was equipped with a tower allowing the introduction of three Tygon tubing to deliver air and odorants. The bottom of the animal chamber was connected to a ventilation pump. An ultrasound microphone was inserted in the tower to monitor ultrasonic vocalizations (USV) emitted by the rats. The floor of the animal chamber has been equipped with a shock-floor allowing delivering scrambled foot-shock. (B) Individual example of raw respiratory signal. I, inspiration phase; E, expiration phase. (C) Individual example of ultrasonic vocalizations. Upper part: waveform of three successive USV. Lower part: corresponding frequency spectrogram.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3101376&req=5

Figure 1: (A) The experimental cage consisted in a customized plethysmograph used to measure respiratory activity in behaving rats. It is composed of two parts: the animal and reference chambers both connected to a differential pressure transducer. The ceiling of the plethysmograph was equipped with a tower allowing the introduction of three Tygon tubing to deliver air and odorants. The bottom of the animal chamber was connected to a ventilation pump. An ultrasound microphone was inserted in the tower to monitor ultrasonic vocalizations (USV) emitted by the rats. The floor of the animal chamber has been equipped with a shock-floor allowing delivering scrambled foot-shock. (B) Individual example of raw respiratory signal. I, inspiration phase; E, expiration phase. (C) Individual example of ultrasonic vocalizations. Upper part: waveform of three successive USV. Lower part: corresponding frequency spectrogram.
Mentions: The apparatus consisted of a whole body customized plethysmograph (Figure 1A; diameter 20 cm, height 30 cm, EMKA technologies, France; see also Figure A1 in Appendix) placed in a home made sound-attenuating cage (L 60 cm, W 60 cm, H 70 cm). The plethysmograph was used to measure respiratory parameters in behaving animals. It is composed of two parts which come together to form two independent airtight chambers: the animal and reference chambers. The pressure change due to animal respiration was measured by a differential pressure transducer (Model dpt, EMKA Technologies, France) with one port exposed to the animal chamber and the other to the reference chamber.

Bottom Line: In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations.Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states.The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

View Article: PubMed Central - PubMed

Affiliation: Team "Olfaction: From Coding to Memory", Lyon Neuroscience Research Center, INSERM U1028, CNRS UMR5292 Lyon, France.

ABSTRACT
In animals, emotional memory is classically assessed through pavlovian fear conditioning in which a neutral novel stimulus (conditioned stimulus) is paired with an aversive unconditioned stimulus. After conditioning, the conditioned stimulus elicits a fear response characterized by a wide range of behavioral and physiological responses. Despite the existence of this large repertoire of responses, freezing behavior is often the sole parameter used for quantifying fear response, thus limiting emotional memory appraisal to this unique index. Interestingly, respiratory changes and ultrasonic vocalizations (USV) can occur during fear response, yet very few studies investigated the link between these different parameters and freezing. The aim of the present study was to design an experimental setup allowing the simultaneous recording of respiration, USV, and behavior (RUB cage), and the offline synchronization of the collected data for fine-grain second by second analysis. The setup consisted of a customized plethysmograph for respiration monitoring, equipped with a microphone capturing USV, and with four video cameras for behavior recording. In addition, the bottom of the plethysmograph was equipped with a shock-floor allowing foot-shock delivery, and the top received tubing for odor presentations. Using this experimental setup we first described the characteristics of respiration and USV in different behaviors and emotional states. Then we monitored these parameters during contextual fear conditioning and showed that they bring complementary information about the animal's anxiety state and the strength of aversive memory. The present setup may be valuable in providing a clearer appraisal of the physiological and behavioral changes that occur during acquisition as well as retrieval of emotional memory.

No MeSH data available.


Related in: MedlinePlus