Investigations on the prevalence and potential pathogenicity of intestinal trichomonads in pigs using in situ hybridization.
Bottom Line: Partial 18S ribosomal RNA gene sequencing of six of those animals showed a 100% sequence identity with T. foetus sequences.The majority of these animals were also tested positive for other enteropathogenic agents, such as Brachyspira sp., Lawsonia intracellularis, Escherichia coli, and porcine circovirus type 2.All OT-positive samples were further examined with another probe complementary to all known Tritrichomonas species sequences including T. foetus, T. augusta, T. mobilensis and T. nonconforma resulting in only 48 positives.
Affiliation: Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.Show MeSH
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Mentions: The presence of intestinal trichomonads with infection of other pathogens such as Brachyspira sp., porcine circovirus type 2, L. intracellularis and E. coli were correlated (data summarized in Fig. 2). In general, the majority of cases with presence of trichomonads also had an infection with other intestinal pathogens. In both, m and h infestation grades only one case out of ten or nine animals, respectively, was negative for all tested pathogens. One case each of both infestation grades, however, was only slightly positive in the PCV-2 ISH, a result which is considered of minor clinical importance.
Affiliation: Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.