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The mouse primary visual cortex is a site of production and sensitivity to estrogens.

Jeong JK, Tremere LA, Burrows K, Majewska AK, Pinaud R - PLoS ONE (2011)

Bottom Line: We found that both monocular and binocular V1 are highly enriched in aromatase- and ER-positive neurons, indicating that V1 is a site of production and sensitivity to estrogens.Interestingly, acute episodes of visual experience do not affect the density or distribution of estrogen-associated circuits.Finally, we show that adult mice dark-reared from birth also exhibit normal distribution of aromatase and ERs throughout V1, suggesting that the implementation and maintenance of estrogen-associated circuits is independent of visual experience.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America.

ABSTRACT
The classic female estrogen, 17β-estradiol (E2), has been repeatedly shown to affect the perceptual processing of visual cues. Although gonadal E2 has often been thought to influence these processes, the possibility that central visual processing may be modulated by brain-generated hormone has not been explored. Here we show that estrogen-associated circuits are highly prevalent in the mouse primary visual cortex (V1). Specifically, we cloned aromatase, a marker for estrogen-producing neurons, and the classic estrogen receptors (ERs) ERα and ERβ, as markers for estrogen-responsive neurons, and conducted a detailed expression analysis via in-situ hybridization. We found that both monocular and binocular V1 are highly enriched in aromatase- and ER-positive neurons, indicating that V1 is a site of production and sensitivity to estrogens. Using double-fluorescence in-situ hybridization, we reveal the neurochemical identity of estrogen-producing and -sensitive cells in V1, and demonstrate that they constitute a heterogeneous neuronal population. We further show that visual experience engages a large population of aromatase-positive neurons and, to a lesser extent, ER-expressing neurons, suggesting that E2 levels may be locally regulated by visual input in V1. Interestingly, acute episodes of visual experience do not affect the density or distribution of estrogen-associated circuits. Finally, we show that adult mice dark-reared from birth also exhibit normal distribution of aromatase and ERs throughout V1, suggesting that the implementation and maintenance of estrogen-associated circuits is independent of visual experience. Our findings demonstrate that the adult V1 is a site of production and sensitivity to estrogens, and suggest that locally-produced E2 may shape visual cortical processing.

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The numerical densities of ARO, ERα or ERß-positive neurons in V1 are not affected by acute visual stimulation.Line graphs depicting the mean (± S.E.) numerical densities of ARO-, ERα and ERβ-positive cells in the V1 of unstimulated (control) adult mice, as well as in animals subjected to 30 min, 1 h or 2 h of ambient light stimulation (see Methods). Data is shown for supragranular (II/III), granular (IV) and infragranular (V/VI) layers of the V1 separately. No significant differences were detected for any of the layers and experimental groups analyzed, for ARO, ERα or ERβ-positive cells.
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pone-0020400-g006: The numerical densities of ARO, ERα or ERß-positive neurons in V1 are not affected by acute visual stimulation.Line graphs depicting the mean (± S.E.) numerical densities of ARO-, ERα and ERβ-positive cells in the V1 of unstimulated (control) adult mice, as well as in animals subjected to 30 min, 1 h or 2 h of ambient light stimulation (see Methods). Data is shown for supragranular (II/III), granular (IV) and infragranular (V/VI) layers of the V1 separately. No significant differences were detected for any of the layers and experimental groups analyzed, for ARO, ERα or ERβ-positive cells.

Mentions: We found that acute visual experience did not affect the population of ARO-positive cells in V1 (Fig. 6; Table 1). Likewise, acute visual stimulation did not impact the population of cells expressing either estrogen receptor across cortical layers of V1 (Fig. 6; Tables 2 and 3). Overall, the findings above indicate that acute visual experience does not affect the numerical densities of ARO- ERα- and ERβ-positive neurons and suggest that estrogen-associated circuits in V1 are highly stable to short epochs of visual stimulation.


The mouse primary visual cortex is a site of production and sensitivity to estrogens.

Jeong JK, Tremere LA, Burrows K, Majewska AK, Pinaud R - PLoS ONE (2011)

The numerical densities of ARO, ERα or ERß-positive neurons in V1 are not affected by acute visual stimulation.Line graphs depicting the mean (± S.E.) numerical densities of ARO-, ERα and ERβ-positive cells in the V1 of unstimulated (control) adult mice, as well as in animals subjected to 30 min, 1 h or 2 h of ambient light stimulation (see Methods). Data is shown for supragranular (II/III), granular (IV) and infragranular (V/VI) layers of the V1 separately. No significant differences were detected for any of the layers and experimental groups analyzed, for ARO, ERα or ERβ-positive cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3101258&req=5

pone-0020400-g006: The numerical densities of ARO, ERα or ERß-positive neurons in V1 are not affected by acute visual stimulation.Line graphs depicting the mean (± S.E.) numerical densities of ARO-, ERα and ERβ-positive cells in the V1 of unstimulated (control) adult mice, as well as in animals subjected to 30 min, 1 h or 2 h of ambient light stimulation (see Methods). Data is shown for supragranular (II/III), granular (IV) and infragranular (V/VI) layers of the V1 separately. No significant differences were detected for any of the layers and experimental groups analyzed, for ARO, ERα or ERβ-positive cells.
Mentions: We found that acute visual experience did not affect the population of ARO-positive cells in V1 (Fig. 6; Table 1). Likewise, acute visual stimulation did not impact the population of cells expressing either estrogen receptor across cortical layers of V1 (Fig. 6; Tables 2 and 3). Overall, the findings above indicate that acute visual experience does not affect the numerical densities of ARO- ERα- and ERβ-positive neurons and suggest that estrogen-associated circuits in V1 are highly stable to short epochs of visual stimulation.

Bottom Line: We found that both monocular and binocular V1 are highly enriched in aromatase- and ER-positive neurons, indicating that V1 is a site of production and sensitivity to estrogens.Interestingly, acute episodes of visual experience do not affect the density or distribution of estrogen-associated circuits.Finally, we show that adult mice dark-reared from birth also exhibit normal distribution of aromatase and ERs throughout V1, suggesting that the implementation and maintenance of estrogen-associated circuits is independent of visual experience.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America.

ABSTRACT
The classic female estrogen, 17β-estradiol (E2), has been repeatedly shown to affect the perceptual processing of visual cues. Although gonadal E2 has often been thought to influence these processes, the possibility that central visual processing may be modulated by brain-generated hormone has not been explored. Here we show that estrogen-associated circuits are highly prevalent in the mouse primary visual cortex (V1). Specifically, we cloned aromatase, a marker for estrogen-producing neurons, and the classic estrogen receptors (ERs) ERα and ERβ, as markers for estrogen-responsive neurons, and conducted a detailed expression analysis via in-situ hybridization. We found that both monocular and binocular V1 are highly enriched in aromatase- and ER-positive neurons, indicating that V1 is a site of production and sensitivity to estrogens. Using double-fluorescence in-situ hybridization, we reveal the neurochemical identity of estrogen-producing and -sensitive cells in V1, and demonstrate that they constitute a heterogeneous neuronal population. We further show that visual experience engages a large population of aromatase-positive neurons and, to a lesser extent, ER-expressing neurons, suggesting that E2 levels may be locally regulated by visual input in V1. Interestingly, acute episodes of visual experience do not affect the density or distribution of estrogen-associated circuits. Finally, we show that adult mice dark-reared from birth also exhibit normal distribution of aromatase and ERs throughout V1, suggesting that the implementation and maintenance of estrogen-associated circuits is independent of visual experience. Our findings demonstrate that the adult V1 is a site of production and sensitivity to estrogens, and suggest that locally-produced E2 may shape visual cortical processing.

Show MeSH