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AC-NP: a novel chimeric peptide with natriuretic and vasorelaxing actions.

Chen BY, Chen JK, Zhu MZ, Zhang DL, Sun JS, Pei JM, Feng HS, Zhu XX, Jin J, Yu J - PLoS ONE (2011)

Bottom Line: In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP.Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP.Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

ABSTRACT
The aim of this study was to evaluate the cardiovascular and renal activities of a newly designed natriuretic peptide (NP). Here, we engineered a novel 28-amino acid chimeric peptide, termed AC-NP that combined the 17-amino acid ring of C type natriuretic peptide (CNP) with the 6-amino acid N-terminus and 5-amino acid C-terminus of atrial natriuretic peptide (ANP). Both in vitro and in vivo experiments were performed to determine the actions of AC-NP. In normal rats, AC-NP proved to be more potentially diuretic, natriuretic and hypotensive compared with other NPs, such as ANP, CNP and vasonatrin peptide (VNP), which is another man-made NP. In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP. Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP. Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

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Related in: MedlinePlus

Effect of AC-NP on mean carotid arterial pressure (mCAP) in rats.The mCAP values were normalized against the corresponding values of baseline, which were arbitrarily set as 1. Data are expressed as means ± SEM, n = 6. *P<0.05 versus baseline. # P<0.05 versus ANP, CNP, or VNP group. Saline was used as control.
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pone-0020477-g003: Effect of AC-NP on mean carotid arterial pressure (mCAP) in rats.The mCAP values were normalized against the corresponding values of baseline, which were arbitrarily set as 1. Data are expressed as means ± SEM, n = 6. *P<0.05 versus baseline. # P<0.05 versus ANP, CNP, or VNP group. Saline was used as control.

Mentions: In accordance with the in vitro evidences, ANP, CNP, VNP or AC-NP at the dose of 10−6 M all induced significant decrease of mCAP in comparison with the level of baseline (P<0.05, n = 6). The decreased percentage of ANP, CNP, VNP and AC-NP was 24%, 15%, 29% and 44% indicating potential hypotensive effects of AC-NP (Fig. 3).


AC-NP: a novel chimeric peptide with natriuretic and vasorelaxing actions.

Chen BY, Chen JK, Zhu MZ, Zhang DL, Sun JS, Pei JM, Feng HS, Zhu XX, Jin J, Yu J - PLoS ONE (2011)

Effect of AC-NP on mean carotid arterial pressure (mCAP) in rats.The mCAP values were normalized against the corresponding values of baseline, which were arbitrarily set as 1. Data are expressed as means ± SEM, n = 6. *P<0.05 versus baseline. # P<0.05 versus ANP, CNP, or VNP group. Saline was used as control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3101257&req=5

pone-0020477-g003: Effect of AC-NP on mean carotid arterial pressure (mCAP) in rats.The mCAP values were normalized against the corresponding values of baseline, which were arbitrarily set as 1. Data are expressed as means ± SEM, n = 6. *P<0.05 versus baseline. # P<0.05 versus ANP, CNP, or VNP group. Saline was used as control.
Mentions: In accordance with the in vitro evidences, ANP, CNP, VNP or AC-NP at the dose of 10−6 M all induced significant decrease of mCAP in comparison with the level of baseline (P<0.05, n = 6). The decreased percentage of ANP, CNP, VNP and AC-NP was 24%, 15%, 29% and 44% indicating potential hypotensive effects of AC-NP (Fig. 3).

Bottom Line: In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP.Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP.Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

ABSTRACT
The aim of this study was to evaluate the cardiovascular and renal activities of a newly designed natriuretic peptide (NP). Here, we engineered a novel 28-amino acid chimeric peptide, termed AC-NP that combined the 17-amino acid ring of C type natriuretic peptide (CNP) with the 6-amino acid N-terminus and 5-amino acid C-terminus of atrial natriuretic peptide (ANP). Both in vitro and in vivo experiments were performed to determine the actions of AC-NP. In normal rats, AC-NP proved to be more potentially diuretic, natriuretic and hypotensive compared with other NPs, such as ANP, CNP and vasonatrin peptide (VNP), which is another man-made NP. In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP. Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP. Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

Show MeSH
Related in: MedlinePlus