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AC-NP: a novel chimeric peptide with natriuretic and vasorelaxing actions.

Chen BY, Chen JK, Zhu MZ, Zhang DL, Sun JS, Pei JM, Feng HS, Zhu XX, Jin J, Yu J - PLoS ONE (2011)

Bottom Line: In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP.Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP.Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

ABSTRACT
The aim of this study was to evaluate the cardiovascular and renal activities of a newly designed natriuretic peptide (NP). Here, we engineered a novel 28-amino acid chimeric peptide, termed AC-NP that combined the 17-amino acid ring of C type natriuretic peptide (CNP) with the 6-amino acid N-terminus and 5-amino acid C-terminus of atrial natriuretic peptide (ANP). Both in vitro and in vivo experiments were performed to determine the actions of AC-NP. In normal rats, AC-NP proved to be more potentially diuretic, natriuretic and hypotensive compared with other NPs, such as ANP, CNP and vasonatrin peptide (VNP), which is another man-made NP. In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP. Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP. Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

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Related in: MedlinePlus

Amino acid sequences and structures of human ANP (h-ANP), human CNP (h-CNP), chimeric VNP and novel chimeric AC-NP.
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Related In: Results  -  Collection


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pone-0020477-g001: Amino acid sequences and structures of human ANP (h-ANP), human CNP (h-CNP), chimeric VNP and novel chimeric AC-NP.

Mentions: Figure 1 illustrates the structures of ANP, CNP, VNP, and the new peptide, AC-NP. AC-NP is 28-amino acid in length including 17-amino acid ringed structure from CNP, as well as both C- and N-termini of ANP. ANP, CNP, VNP and AC-NP were all synthesized by School of Medicine and Pharmaceutics, Jiangnan University (Wuxi, China). L-norepinephrine bitartrate (NE) and acetylcholine chloride (Ach) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). [125I]-cGMP assay kit was from Shanghai Chinese Medical University (Shanghai, China).


AC-NP: a novel chimeric peptide with natriuretic and vasorelaxing actions.

Chen BY, Chen JK, Zhu MZ, Zhang DL, Sun JS, Pei JM, Feng HS, Zhu XX, Jin J, Yu J - PLoS ONE (2011)

Amino acid sequences and structures of human ANP (h-ANP), human CNP (h-CNP), chimeric VNP and novel chimeric AC-NP.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3101257&req=5

pone-0020477-g001: Amino acid sequences and structures of human ANP (h-ANP), human CNP (h-CNP), chimeric VNP and novel chimeric AC-NP.
Mentions: Figure 1 illustrates the structures of ANP, CNP, VNP, and the new peptide, AC-NP. AC-NP is 28-amino acid in length including 17-amino acid ringed structure from CNP, as well as both C- and N-termini of ANP. ANP, CNP, VNP and AC-NP were all synthesized by School of Medicine and Pharmaceutics, Jiangnan University (Wuxi, China). L-norepinephrine bitartrate (NE) and acetylcholine chloride (Ach) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). [125I]-cGMP assay kit was from Shanghai Chinese Medical University (Shanghai, China).

Bottom Line: In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP.Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP.Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

ABSTRACT
The aim of this study was to evaluate the cardiovascular and renal activities of a newly designed natriuretic peptide (NP). Here, we engineered a novel 28-amino acid chimeric peptide, termed AC-NP that combined the 17-amino acid ring of C type natriuretic peptide (CNP) with the 6-amino acid N-terminus and 5-amino acid C-terminus of atrial natriuretic peptide (ANP). Both in vitro and in vivo experiments were performed to determine the actions of AC-NP. In normal rats, AC-NP proved to be more potentially diuretic, natriuretic and hypotensive compared with other NPs, such as ANP, CNP and vasonatrin peptide (VNP), which is another man-made NP. In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP. Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP. Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.

Show MeSH
Related in: MedlinePlus