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Prophylactic effect of a therapeutic vaccine against TB based on fragments of Mycobacterium tuberculosis.

Vilaplana C, Gil O, Cáceres N, Pinto S, Díaz J, Cardona PJ - PLoS ONE (2011)

Bottom Line: RUTI® protected the spleen less than BCG.Following a 9 month vaccination-challenge interval, protection was observed for the lungs, but not for the spleen.Inoculations of RUTI® shortly after infection significantly reduced the viable bacterial counts in the lungs, when compared with infected control mice.

View Article: PubMed Central - PubMed

Affiliation: Unitat de Tuberculosi Experimental, Fundació Institut per a la Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, Crta Badalona, Catalonia, Spain.

ABSTRACT
The prophylactic capacity of the RUTI® vaccine, based on fragmented cells of Mycobacterium tuberculosis, has been evaluated in respect to aerosol challenge with virulent bacilli. Subcutaneous vaccination significantly reduced viable bacterial counts in both lungs and spleens of C57Bl mice, when challenged 4 weeks after vaccination. RUTI® protected the spleen less than BCG. Following a 9 month vaccination-challenge interval, protection was observed for the lungs, but not for the spleen. Survival of infected guinea pigs was prolonged by vaccination given 5 weeks before challenge. Inoculations of RUTI® shortly after infection significantly reduced the viable bacterial counts in the lungs, when compared with infected control mice. Thus, vaccination by RUTI® has potential for both the prophylaxis and immunotherapy of tuberculosis.

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Related in: MedlinePlus

Vaccination soon after infection.The figure shows the protection induced by vaccinating after challenge. The experimental groups are shown as follows: black (unvaccinated animals, control group), white (BCG day 4), horizontal striped (RUTI® day 4), diagonal striped (RUTI® day 11) and chequered (RUTI® day 4 and 11). Significant differences (p<0.05) respect to the control group are marked with *.
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pone-0020404-g004: Vaccination soon after infection.The figure shows the protection induced by vaccinating after challenge. The experimental groups are shown as follows: black (unvaccinated animals, control group), white (BCG day 4), horizontal striped (RUTI® day 4), diagonal striped (RUTI® day 11) and chequered (RUTI® day 4 and 11). Significant differences (p<0.05) respect to the control group are marked with *.

Mentions: The results (Figure 4) showed that RUTI decreased the bacillary load in mice lungs when given at day 4 (reduction of 0.53 logs) or at day 4 plus 11 (reduction of 1.03 logs) post challenge in a statistically significant way when compared to the infected and the BCG-vaccinated groups (One-Way Anova, p<0.005). No statistically significant differences where encountered in the spleen.


Prophylactic effect of a therapeutic vaccine against TB based on fragments of Mycobacterium tuberculosis.

Vilaplana C, Gil O, Cáceres N, Pinto S, Díaz J, Cardona PJ - PLoS ONE (2011)

Vaccination soon after infection.The figure shows the protection induced by vaccinating after challenge. The experimental groups are shown as follows: black (unvaccinated animals, control group), white (BCG day 4), horizontal striped (RUTI® day 4), diagonal striped (RUTI® day 11) and chequered (RUTI® day 4 and 11). Significant differences (p<0.05) respect to the control group are marked with *.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3101251&req=5

pone-0020404-g004: Vaccination soon after infection.The figure shows the protection induced by vaccinating after challenge. The experimental groups are shown as follows: black (unvaccinated animals, control group), white (BCG day 4), horizontal striped (RUTI® day 4), diagonal striped (RUTI® day 11) and chequered (RUTI® day 4 and 11). Significant differences (p<0.05) respect to the control group are marked with *.
Mentions: The results (Figure 4) showed that RUTI decreased the bacillary load in mice lungs when given at day 4 (reduction of 0.53 logs) or at day 4 plus 11 (reduction of 1.03 logs) post challenge in a statistically significant way when compared to the infected and the BCG-vaccinated groups (One-Way Anova, p<0.005). No statistically significant differences where encountered in the spleen.

Bottom Line: RUTI® protected the spleen less than BCG.Following a 9 month vaccination-challenge interval, protection was observed for the lungs, but not for the spleen.Inoculations of RUTI® shortly after infection significantly reduced the viable bacterial counts in the lungs, when compared with infected control mice.

View Article: PubMed Central - PubMed

Affiliation: Unitat de Tuberculosi Experimental, Fundació Institut per a la Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, Crta Badalona, Catalonia, Spain.

ABSTRACT
The prophylactic capacity of the RUTI® vaccine, based on fragmented cells of Mycobacterium tuberculosis, has been evaluated in respect to aerosol challenge with virulent bacilli. Subcutaneous vaccination significantly reduced viable bacterial counts in both lungs and spleens of C57Bl mice, when challenged 4 weeks after vaccination. RUTI® protected the spleen less than BCG. Following a 9 month vaccination-challenge interval, protection was observed for the lungs, but not for the spleen. Survival of infected guinea pigs was prolonged by vaccination given 5 weeks before challenge. Inoculations of RUTI® shortly after infection significantly reduced the viable bacterial counts in the lungs, when compared with infected control mice. Thus, vaccination by RUTI® has potential for both the prophylaxis and immunotherapy of tuberculosis.

Show MeSH
Related in: MedlinePlus