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Anti-tumor activity of a novel protein obtained from tartary buckwheat.

Guo X, Zhu K, Zhang H, Yao H - Int J Mol Sci (2010)

Bottom Line: Cell-cycle analysis revealed that treatment with TBWSP31 resulted in a G(0)/G(1) arrest and prevented the cells from growing from G(0)/G(1) phase to S phase, which was most prominent at 48 h.The expression of bcl-2 and Fas were detected quantitatively by FCM, which showed that TBWSP31 induced-apoptosis may be involved with the participation of Fas and bcl-2.These results suggest that TBWSP31 is a potential antitumor compound and that apoptosis induced by TBWSP31 is a key antitumor mechanism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi-214122, Jiangsu, China; E-Mails: zkx1978@hotmail.com (K.Z.); hui1003@hotmail.com (H.Z.); hyyao@jiangnan.edu.cn (H.Y.).

ABSTRACT
TBWSP31 is a novel antitumor protein that was isolated from tartary buckwheat water-soluble extracts. The objective of this paper was to investigate the anti-proliferative effects of TBWSP31 on breast cancer Bcap37cells and to explore its possible mechanism. After treatment of Bcap37 cells with TBWSP31, typical apoptotic morphological changes were observed by inverted microscopy and scanning electron microscopy (SEM), such as detachment from the culture plate, change to a round shape, cell shrinkage, the absence of obvious microvilli, plasma membrane blebbing, and formation of apoptotic bodies. Cell-cycle analysis revealed that treatment with TBWSP31 resulted in a G(0)/G(1) arrest and prevented the cells from growing from G(0)/G(1) phase to S phase, which was most prominent at 48 h. The expression of bcl-2 and Fas were detected quantitatively by FCM, which showed that TBWSP31 induced-apoptosis may be involved with the participation of Fas and bcl-2. These results suggest that TBWSP31 is a potential antitumor compound and that apoptosis induced by TBWSP31 is a key antitumor mechanism.

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The morphological changes of Bcap37 cells as observed by scanning electron microscopy (SEM; 8000×). Two images of Bcap37 cells cultured with 0 μg/mL (A) or 20 μg/mL (B) of TBWSP31 for 48 h.
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f3-ijms-11-05201: The morphological changes of Bcap37 cells as observed by scanning electron microscopy (SEM; 8000×). Two images of Bcap37 cells cultured with 0 μg/mL (A) or 20 μg/mL (B) of TBWSP31 for 48 h.

Mentions: To further investigate whether TBWSP31 mediated cell death of Bcap37 cells is due to an apoptotic mechanism, the surface morphological changes that occurred with 20 μg/mL of TBWSP31 treatment for 48 h were observed under SEM. Figure 3 shows the morphological changes of Bcap37 cells observed by SEM.


Anti-tumor activity of a novel protein obtained from tartary buckwheat.

Guo X, Zhu K, Zhang H, Yao H - Int J Mol Sci (2010)

The morphological changes of Bcap37 cells as observed by scanning electron microscopy (SEM; 8000×). Two images of Bcap37 cells cultured with 0 μg/mL (A) or 20 μg/mL (B) of TBWSP31 for 48 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100852&req=5

f3-ijms-11-05201: The morphological changes of Bcap37 cells as observed by scanning electron microscopy (SEM; 8000×). Two images of Bcap37 cells cultured with 0 μg/mL (A) or 20 μg/mL (B) of TBWSP31 for 48 h.
Mentions: To further investigate whether TBWSP31 mediated cell death of Bcap37 cells is due to an apoptotic mechanism, the surface morphological changes that occurred with 20 μg/mL of TBWSP31 treatment for 48 h were observed under SEM. Figure 3 shows the morphological changes of Bcap37 cells observed by SEM.

Bottom Line: Cell-cycle analysis revealed that treatment with TBWSP31 resulted in a G(0)/G(1) arrest and prevented the cells from growing from G(0)/G(1) phase to S phase, which was most prominent at 48 h.The expression of bcl-2 and Fas were detected quantitatively by FCM, which showed that TBWSP31 induced-apoptosis may be involved with the participation of Fas and bcl-2.These results suggest that TBWSP31 is a potential antitumor compound and that apoptosis induced by TBWSP31 is a key antitumor mechanism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi-214122, Jiangsu, China; E-Mails: zkx1978@hotmail.com (K.Z.); hui1003@hotmail.com (H.Z.); hyyao@jiangnan.edu.cn (H.Y.).

ABSTRACT
TBWSP31 is a novel antitumor protein that was isolated from tartary buckwheat water-soluble extracts. The objective of this paper was to investigate the anti-proliferative effects of TBWSP31 on breast cancer Bcap37cells and to explore its possible mechanism. After treatment of Bcap37 cells with TBWSP31, typical apoptotic morphological changes were observed by inverted microscopy and scanning electron microscopy (SEM), such as detachment from the culture plate, change to a round shape, cell shrinkage, the absence of obvious microvilli, plasma membrane blebbing, and formation of apoptotic bodies. Cell-cycle analysis revealed that treatment with TBWSP31 resulted in a G(0)/G(1) arrest and prevented the cells from growing from G(0)/G(1) phase to S phase, which was most prominent at 48 h. The expression of bcl-2 and Fas were detected quantitatively by FCM, which showed that TBWSP31 induced-apoptosis may be involved with the participation of Fas and bcl-2. These results suggest that TBWSP31 is a potential antitumor compound and that apoptosis induced by TBWSP31 is a key antitumor mechanism.

Show MeSH
Related in: MedlinePlus